YANG Zeliang,REN Jianhang,KUANG Yuanyuan,et al.Dynamics of Soil Microbial Communities Along Vegetation Restoration Gradient in Karst Area[J].Research of Soil and Water Conservation,2019,26(03):185-191.
桂西北喀斯特不同植被演替阶段土壤微生物群落多样性
- Title:
- Dynamics of Soil Microbial Communities Along Vegetation Restoration Gradient in Karst Area
- 分类号:
- X171
- 摘要:
- 采用经典统计与排序分析方法，研究了桂西北喀斯特不同植被演替阶段（原生林、次生林、灌丛、草地）土壤微生物群落多样性变化特征，并探讨了土壤微生物群落多样性与土壤养分、土壤微生物生物量和土壤微生物数量之间的关系。结果表明：（1）桂西北喀斯特植被演替对土壤养分有较大的影响，除了土壤容重和全磷含量以外，由草地到原生林的演替过程中，土壤养分均呈显著的变化趋势（p<0.05）。随着演替的进行，土壤pH值和容重逐渐减小。土壤有机碳、全氮、全钾、速效氮、速效磷和速效钾含量随着演替而逐渐增加趋势，其大小依次变现为原生林 > 次生林 > 灌丛 > 草地，其中不同演替阶段土壤有机碳全氮、速效氮、速效磷和速效钾含量差异均显著（p<0.05）。（2）土壤微生物量碳、氮、磷大小依次变现为原生林 > 次生林 > 灌丛 > 草地，其中灌丛和次生林MBP含量差异不显著（p>0.05）。MBC/MBN随着演替的进行呈逐渐减小趋势，MBC/MBP和MBN/MBP随着演替的进行呈逐渐增加趋势。（3）不同植被演替阶段土壤微生物数量及组成不同，其中以细菌数目最多，土壤微生物种群总数量、细菌和放线菌数目依次为原生林>次生林>灌丛>草地。（4）不同演替阶段土壤微生物群落功能多样性指数存在一定差异，其中物种丰富度指数（H）、均匀度指数（E）和碳源利用丰富度指数（S）依次表现为原生林 > 次生林 > 灌丛 > 草地（p<0.05）；而优势度指数（Ds）依次表现为原生林 < 次生林 < 灌丛 < 草地，并且不同演替阶段优势度指数（Ds）差异不显著（p>0.05）。（5）相关性分析表明土壤养分、土壤微生物量、土壤微生物数量均与土壤微生物群落多样性具有显著的相关性，其中，土壤微生物量对微生物群落多样性的贡献最大，而MBN，SOC和细菌数目是影响桂西北喀斯特不同植被演替阶段土壤微生物群落多样性分布的主要因子。
- Abstract:
- We presented a comprehensive analysis of soil microbial communities and soil nutrients along a vegetation restoration gradient in a karst area, and explored the relationship between soil microbial communities and soil nutrients. The findings are as followings. (1) Vegetation restoration had strong effect on soil nutrients(p<0.05), soil nutrients gradually decreased in the order of primeval forest > secondary forest > shrubland > grassland, while soil pH and soil bulk density gradually decreased. (2) Soil microbial biomass carbon, nitrogen and phosphorus decreased in the order:primeval forest > secondary forest > shrubland > grassland, and there was no significantly difference between secondary forest and shrubland. MBC/MBN gradually decreased and BC/MBP, MBN/MBP gradually increased with the vegetation restoration development. (3) There was a large number of bacteria in different vegetation restoration, and the number of the total soil microbes, bacteria and fungi decreased in the sequence:primeval forest > secondary forestland > shrubland > grassland. (4) Vegetation restoration had strong effect on soil microbial communities, species richness index (H), evenness index (E) and carbon source utilization richness index (S) decreased in the order:primeval forest > secondary forest > shrubland > grassland, while dominance index (Ds) showed the opposite change trend with no significant difference (p>0.05). (5) Correlation analysis showed that soil nutrient, soil microorganism quantity and soil microorganism quantity were significantly related to soil microbial diversity, and soil microbial biomass had the important contribution to soil microbial diversity, while MBN, SOC and the number of bacteria can be regard as the main driving factors on the pattern of soil microbial community in a karst area.
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备注/Memo
收稿日期:2018-08-14;改回日期:2018-09-12。
基金项目:湖南省自然科学基金项目（13JJ4105）
作者简介:杨泽良(1977-),男,湖南衡阳人,博士,讲师,研究方向为植物生理生态学。E-mail:Zeliang_yang@126.com