CHEN Xin,XU Guoce,LI Qingshun,et al.Functional Diversity of Soil Microbial Communities in the Water-Level-Fluctuating Zone of Dali River[J].Research of Soil and Water Conservation,2023,30(05):193-200.[doi:10.13869/j.cnki.rswc.2023.05.023.]
大理河消落带土壤微生物群落功能多样性
- Title:
- Functional Diversity of Soil Microbial Communities in the Water-Level-Fluctuating Zone of Dali River
- 文章编号:
- 1005-3409(2023)05-0193-08
- 关键词:
- 大理河; 消落带; 土壤微生物; Biolog-Eco; 功能多样性
- Keywords:
- Dali River; water-level-fluctuating zone; soil microorganism; Biolog-Eco; functional diversity
- 分类号:
- S154.3
- 文献标志码:
- A
- 摘要:
- [目的]研究大理河消落带土壤微生物群落功能多样性,可以从微生物的角度为河流生态保护提供新的理论依据和数据支持。[方法]以大理河消落带0—5 cm土壤为对象,采用Biolog-ECO微平板法研究了消落带土壤微生物平均颜色变化率(AWCD)、土壤微生物对不同碳源的利用特征以及土壤微生物群落多样性指数。[结果]大理河消落带AWCD均值为中游>上游>下游,中游土壤微生物群落利用碳源的能力更强。羧酸类、胺类、酚酸类和聚合物类4种碳源是上游土壤微生物利用的主要碳源,胺类和聚合物类是中游和下游土壤微生物利用的主要碳源,总体来看,胺类和聚合物类为大理河消落带土壤微生物利用的主要碳源。土壤微生物Shannon多样性指数和Simpson优势度指数最大的是下游。土壤粉粒、黏粒、全氮和pH值对微生物群落的多样性有显著影响(p<0.05)。[结论]大理河消落带土壤微生物群落功能多样性在上、中、下游存在差异,且微生物群落多样性受土壤理化性质的影响。黄河消落带的保护不仅要着眼于土壤理化性质的变化,更要发现微生物生态功能的差异。
- Abstract:
- [Objective] Studying the functional diversity of soil microbial communities in the water-level-fluctuating zone of Dali River can provide a new theoretical basis and data support for river ecological protection from the perspective of microorganisms. [Methods] The 0—5 cm soil in the water-level fluctuating zone of the Dali River was taken as the object, the Biolog-ECO micro-plate method was used to study the average well color development(AWCD)of soil microorganisms in the water-level fluctuating zone, the utilization characteristics of different carbon sources by soil microorganisms, and the soil microbial community diversity index. [Results] The mean AWCD value of the water-level-fluctuating zone of Dali River decreases in the order: midstream>upstream>downstream. Carboxylic acids, amines, phenolic acids and polymerase are the main carbon sources used by soil microorganisms in the upstream. Amines and polymers are the main carbon sources used by soil microorganisms in the midstream and downstream. Generally speaking, amines and polymers are the main carbon sources used by soil microorganisms in the water-fluctuating-zone of Dali River. The capacity of soil microbial communities to use a single carbon source decreases in the order: upstream>midstream>downstream. Soil particles, total nitrogen, pH, and organic carbon have significant effect on the carbon source utilization capacity of microbial communities(p<0.05). [Conclusion] The functional diversity of soil microbial communities in the water-level-fluctuating zone of Dali River is different in the upper, middle, and lower reaches, and the diversity of microbial communities is affected by soil physical and chemical properties. The protection of the water-fluctuating-zone of the Yellow River should not only focus on changes in soil physical and chemical properties, but also find differences in microbial ecological functions.
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备注/Memo
收稿日期:2022-07-18 修回日期:2022-08-11
资助项目:国家自然科学基金优青项目(No.52022081); 榆林市科技计划项目(No.2019-144)
第一作者:陈新(1998—),女,四川达州人,硕士研究生,主要从事水沙过程演变与流域管理的研究。E-mail:2275233636@qq.com
通信作者:徐国策(1985—),男,河南洛阳人,教授,主要从事流域水沙与污染防治等方面的研究。E-mail:xuguoce_x@163.com