WANG Yingnan,PENG Xiaoyuan,HUA Xiaoyu,et al.Effects of Nitrogen Deposition on the Ion Balance and Organic Acids of the Leymus chinensis-AM Symbiont Roots Under Saline-Alkali Stress[J].Research of Soil and Water Conservation,2019,26(02):118-125.
氮素与盐碱胁迫互作对羊草-丛枝菌根共生体根系离子与有机酸含量的影响
- Title:
- Effects of Nitrogen Deposition on the Ion Balance and Organic Acids of the Leymus chinensis-AM Symbiont Roots Under Saline-Alkali Stress
- Keywords:
- Leymus chinensis; arbuscular mycorrhizal fungi; nitrogen deposition; salinity-alkalinity stress; organic acid
- 分类号:
- S501
- 摘要:
- 为明确氮沉降与盐碱胁迫互作对羊草—丛枝菌根共生体根系渗透调节与离子转运的影响，利用盆栽试验分析了羊草根系中无机离子与有机酸含量的变化。结果表明：随着盐、碱胁迫浓度的增加，羊草根中积累了大量的Na+，Ca2+和Mg2+，并抑制了对K+的吸收，与此同时盐胁迫下积累了大量的Cl-来维持根部的渗透与离子平衡，而碱胁迫下Cl-含量变化不大。接种AM真菌一定程度上降低了Na+的积累，并缓解了K+含量的降低，提高NO3-含量从而改善羊草根部的离子平衡。在氮沉降下，Na+含量有所增加，且K+含量降低，使羊草—丛枝菌根共生体对盐碱胁迫抵抗能力减弱。盐胁迫对羊草根中有机酸含量变化影响很小，而碱胁迫则使有机酸含量大幅提高，接种AM真菌使羊草根中有机酸含量呈下降趋势。上述研究结果表明，接种AM真菌可以显著提高羊草根的抗盐碱能力，而氮沉降的增加对丛枝菌根共生体的耐盐碱能力产生了一定程度的削弱，且铵态氮抑制作用更为显著。研究结果为探求羊草—丛枝菌根共生体对氮沉降及盐碱胁迫的响应和反馈提供一定的理论依据。
- Abstract:
- In order to clarify the effects of the interaction of nitrogen deposition and salt-alkali stress on Leymus chinensis-arbuscular mycorrhizal symbiont osmotic adjustment and ion transport, the pot control experiments were conduced to analyze the changes of inorganic ion and organic acid content in Leymus chinensis roots. The results showed that with the improvement of salt-alkali stresses, the roots of Leymus chinensis seedling accumulated a lot of Na+, Ca2+ and Mg2+, and also inhibited the absorption of K+. Meanwhile, it has accumulated a large amount of Cl- to maintain the osmotic and ionic balance in roots. Inoculation with AM fungi reduced the accumulation of Na+, and the decrease of K+ content was relieved under stresses, improving the content of NO3- to improve the ion balance of Leymus chinensis. Na+ content has increased under nitrogen deposition and resulted in the decreased resistance of Leymus chinensis-arbuscular mycorrhizal symbiosis to salt stress. Salt stress has little effect on organic acid content of Leymus chinensis seedling roots. Under alkaline stress, the content of organic acid increased. Inoculation of AM fungi decreased the content of organic acid in seeding roots. The inoculation with AM fungi could significantly improve the resistance of Leymus chinensis seedling roots. The increase of nitrogen deposition partially weakens the salinity tolerance of arbuscular mycorrhizal symbiosis, and the effect of ammonium nitrogen deposition is more significant. The results can provide certain theoretical basis for the response and feedback of the interaction of nitrogen deposition and salt-alkali stress to Leymus chinensis—AM symbiosis.
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备注/Memo
收稿日期:2018-06-05;改回日期:2018-07-05。
基金项目:国家自然科学基金“氮沉降影响羊草—丛枝菌根共生体适应盐碱逆境的生理机制解析”（31502013）
作者简介:王英男(1989-),男,黑龙江哈尔滨人,博士研究生,研究方向为植物生理与生态学。E-mail:yingnan1027@foxmail.com
通讯作者:蔺吉祥(1985-),男,黑龙江哈尔滨人,副教授,博士,主要从事植物逆境生理生态研究。E-mail:jixiang851012@gmail.com