[1]张帅发,刘峰,褚保森,等.拉萨河流域不同海拔梯度土壤酶化学计量特征[J].水土保持研究,2025,32(05):143-151.[doi:10.13869/j.cnki.rswc.2025.05.001]
　Zhang Shuaifa,Liu Feng,Chu Baosen,et al.Soil enzymatic stoichiometric characteristics at different altitudinal gradients in Lhasa River Basin[J].Research of Soil and Water Conservation,2025,32(05):143-151.[doi:10.13869/j.cnki.rswc.2025.05.001]
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拉萨河流域不同海拔梯度土壤酶化学计量特征

《水土保持研究》[ISSN:1005-3409/CN:61-1272/P] 卷: 32 期数: 2025年05期 页码: 143-151 栏目: 出版日期: 2025-10-10
Title:
Soil enzymatic stoichiometric characteristics at different altitudinal gradients in Lhasa River Basin
文章编号:
1005-3409（2025）05-0143-09
作者:
张帅发1,2,3，刘峰1,2，褚保森1,2，张鹏程1,2，张智勇1,2，陆子淳1,2，王玉婷4，格桑曲珍4，周金星1,2,3
（1.北京林业大学 水土保持学院，云南建水生态站，北京 100083；2.北京林业大学 教育部林业生态工程研究中心，北京 100083；3.北京林业大学林木资源高效生产全国重点实验室，北京 100083；4.西藏自治区林木科学研究院，拉萨 850000）
Author(s):
Zhang Shuaifa1,2,3, Liu Feng1,2, Chu Baosen1,2, Zhang Pengcheng1,2, Zhang Zhiyong1,2, Lu Zichun1,2, Wang Yuting4, Quzhen Gesang4, Zhou Jinxing1,2,3
(1.Jianshui Research Station,School of Soil and Water Conservation,Beijing Forestry University, Beijing 100083,China; 2.Engineering Research Center of Forestry Ecological Engineering of Ministry of Education,Beijing Forestry University,Beijing 100083,China; 3.State Key Laboratory of Efficient Production of Forest Resources,Beijing Forestry University,Beijing 100083,China; 4.Xizang Institute of Forest Trees,Lhasa 850000,China)
关键词:
土壤酶活性; 化学计量; 养分限制; 海拔梯度; 拉萨河流域
Keywords:
soil enzyme activity;  stoichiometry;  nutrient limitation;  altitudinal gradient;  Lhasa River Basin
分类号:
S714.5
DOI:
10.13869/j.cnki.rswc.2025.05.001
文献标志码:
A
摘要:
[目的] 明确土壤酶化学计量特征对海拔梯度的响应关系，并探究环境驱动要素的重要性，为深化高寒高海拔山区森林生态系统中土壤生物地球化学循环过程提供科学依据与数据支撑。[方法] 基于拉萨河流域南山地区5个海拔梯度（3 700~4 100 m）的油松（Pinus tabuliformis）人工林下土壤，探讨了其土壤理化因子、胞外酶活性及微生物养分限制的海拔分异。[结果]（1）海拔显著影响了土壤酶活性的分布格局，纤维素水解酶（CBH）、β-1，4-N-乙酰-氨基葡萄糖苷酶（NAG）、亮氨酸氨基肽酶（LAP）和碱性磷酸酶（AKP）均呈现随海拔升高先增加后降低的趋势，且与相对应的养分显著相关（p<0.05）。（2）VA和VL均呈现出随海拔降低的趋势，低海拔（3 800 m以下）和高海拔（4 000 m以上）区域分别存在相对较强的碳和氮限制。（3）全磷（TP）和微生物生物量碳（MBC）对酶活性及化学计量比的变异贡献率较高（44%和25.6%），是影响胞外酶活性及化学计量比随海拔变化的关键因素。[结论] 土壤酶活性及化学计量比具有显著的垂直空间梯度分异性，微生物营养限制是影响酶化学计量特征的重要因素。
Abstract:
[Objective] To clarify the response relationship of soil enzymatic stoichiometric characteristics to altitudinal gradients and explore the importance of environmental driving factors, aiming to provide scientific evidence and data support for understanding soil biogeochemical cycling processes in high-altitude mountainous forest ecosystems. [Methods] Based on soil samples collected from five altitudinal gradients(3 700~4 100 m) in Pinus tabuliformis plantations in the Nanshan region of the Lhasa River Basin, the spatial differentiation of soil physicochemical factors, extracellular enzyme activities, and microbial nutrient limitations along altitudinal gradients were examined. [Results] (1) Altitude significantly affected the distribution patterns of soil enzyme activities. The activities of cellobiosidase (CBH), β -1, 4-N-acetylglucosaminidase (NAG), L-leucine aminopeptidase (LAP), and alkaline phosphatase (AKP) increased initially, and then decreased with altitude, significantly correlating with their corresponding nutrient contents (p<0.05). (2) The enzyme stoichiometric vector angle(VA) and vector length(VL) exhibited a decreasing trend with lower altitudes, indicating relatively stronger carbon and nitrogen limitations in low-altitude (below 3 800 m) and high-altitude (above 4 000 m) regions.(3) Total phosphorus(TP) and microbial biomass carbon(MBC) contributed the most to variations in enzyme activities and stoichiometric ratios(44% and 25.6%, respectively), making them key factors affecting the variations in extracellular enzyme activity and stoichiometric ratio with altitude. [Conclusion] Soil enzyme activities and stoichiometric ratios exhibit significant vertical spatial differentiation, with microbial nutrient limitation playing a crucial role in shaping enzymatic stoichiometric characteristics.

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备注/Memo

收稿日期：2024-11-29 修回日期：2024-12-23 接受日期：2025-01-13
资助项目：西藏自治区重点研发计划“高寒高海拔地区国土绿化共性关键技术研究”（XZ202301ZY0012G）；西藏自治区重大科技专项“人工林碳汇调查与评估”（XZ202201ZD0005G04）
第一作者：张帅发（2000—），男，河北邯郸人，硕士研究生，研究方向：生态修复学。E-mail：Ssssf9925@163.com
通信作者：周金星（1972—），男，湖南常德人，教授，博士，研究方向：水土保持与荒漠化防治、石漠化治理与生态修复。E-mail：bjfuzjx@126.com

更新日期/Last Update: 2025-10-10