Xu Jiayi,Wang Qiaohong,Ma Cheng,et al.Impact of different artificial grass species on soil enzyme activity and enzyme stoichiometry in alpine regions[J].Research of Soil and Water Conservation,2025,32(01):73-81.[doi:10.13869/j.cnki.rswc.2025.01.037]
高寒地区不同人工牧草种类对土壤酶活性及酶化学计量比的影响
- Title:
- Impact of different artificial grass species on soil enzyme activity and enzyme stoichiometry in alpine regions
- 文章编号:
- 1005-3409(2025)01-0073-09
- Keywords:
- Qinghai-Tibet Plateau; artificial grass planting land; soil enzyme activity; soil enzyme stoichiometry
- 分类号:
- S154.2
- 文献标志码:
- A
- 摘要:
- [目的]探究青藏高原高寒草甸区不同人工牧草土壤酶活性及其化学计量特征,为深入认识高寒地区土壤养分状况及草地生态系统功能提供科学依据。[方法]以冷地早熟禾(Poa crymophila)、无芒披碱草(Elymus submuticus)、短芒披碱草(Elymus breviaristatus)、垂穗披碱草(Elymus nutans)、星星草(Puccinellia tenuiflora)、同德老芒麦(Elymus sibiricus)、贫花鹅观草(Roegneria pauciflora)7种人工牧草种植地土壤为研究对象,并以邻近的天然温带草原土壤作为对照(CK),探讨与土壤碳、氮和磷循环有关酶活性及其化学计量学特征。[结果](1)不同牧草种植地土壤酶活性差异较大〔(3～496 nmol/(g·h)〕,7种牧草土壤酶的活性均明显低于CK〔13～506 nmol/(g·h)〕。(2)土壤碳氮酶活性比(EC:N)在各样地之间无显著差异,冷地早熟禾样地的碳磷酶活性比(EC:P)和氮磷酶活性比(EN:P)最大(0.82, 0.94); 7种牧草土壤酶活性矢量角度均大于45°,表明牧区土壤微生物受到P元素限制。(3)土壤酶活性及其化学计量主要受土壤SOC,TN,W,MBC和MBN的影响,与MBC:MBN,SOC:TP和TN:TP也显著相关。[结论]人工牧草的种植改变了土壤养分结构从而影响土壤酶的分泌,不同牧草种类种植地土壤酶活性及其化学计量存在显著差异。未来结合土壤微生物群落组成以及功能特征可进一步揭示青藏高原高寒草甸土壤养分循环及草地生态系统功能的影响机制。
- Abstract:
- [Objective]The aims of this study are to explore the characteristics of soil enzyme activity and its chemical stoichiometry in alpine meadows of the Qinghai-Tibet Plateau under different artificial grass species, and provide scientific basis for a deeper understanding of soil nutrient status and grassland ecosystem function in the alpine pastoral areas. [Methods]Soils in seven different artificial grass planting including Poa crymophila, Elymus submuticus, Elymus breviaristatus, Elymus nutans, Puccinellia tenuiflora, Elymus sibiricus, and Roegneria pauciflora, were chosen as research objects, and the adjacent natural temperate grassland was used as a control(CK). The enzyme activity and chemical stoichiometry related to soil carbon, nitrogen and phosphorus cycles were investigated. [Results](1)The soil enzyme activity in different pasture planting sites was significantly different, which was in the range of 3～496 nmol/(g·h), and the soil enzyme activities of the seven pasture species were significantly lower than that of the control at 13～506 nmol/(g·h).(2)No significant difference in soil carbon/nitrogen enzyme ratio(EC:N)was observed among the different sites, and the carbon/phosphorus enzyme ratio(EC:P)at 0.82 and nitrogen/phosphorus enzyme ratio(EN:P)at 0.94 were the highest in the plot of Poa crymophila. The vector angles of soil enzyme activities for all seven grass types were greater than 45°, indicating that soil microorganisms in the pastoral areas were limited by phosphorus.(3)Soil enzyme activities and its chemical stoichiometry were mainly influenced by soil organic carbon, total nitrogen, soil moisture, microbial biomass carbon, and microbial biomass nitrogen, were also significantly related to MBC:MBN, SOC:TP and TN:TP. [Conclusion]The cultivation of artificial pasture has changed the nutrient structure of soil and affected the secretion of soil enzymes. There are significant differences in soil enzyme activity and chemical stoichiometry among different pasture species planting sites. In the future, the composition and functional characteristics of soil microbial communities can be further revealed to elucidate the mechanisms of nutrient cycling and grassland ecosystem function in high-altitude alpine meadow soils of the Qinghai-Tibet Plateau.
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备注/Memo
收稿日期:2024-04-29 修回日期:2024-05-07
资助项目:国家自然科学基金(42171301); 陕西省创新人才推进计划科技创新团队项目(2023-CX-TD-37)
第一作者:徐嘉翊(1997—),女,山东潍坊人,硕士研究生,研究方向为土壤微生物学。E-mail:xjyszbd328@163.com
通信作者:薛萐(1978—),男,陕西西安人,博士,研究员,主要从事土壤微生物和生态恢复研究。E-mail:xuesha100@163.com