Xu Jiayi,Wang Qiaohong,Ma Cheng,et al.Impact of different artificial grass species on soil enzyme activity and enzyme stoichiometry in alpine regions[J].Research of Soil and Water Conservation,2025,32(01):73-81.[doi:10.13869/j.cnki.rswc.2025.01.037]
高寒地区不同人工牧草种类对土壤酶活性及酶化学计量比的影响
- Title:
- Impact of different artificial grass species on soil enzyme activity and enzyme stoichiometry in alpine regions
- 文章编号:
- 1005-3409(2025)01-0073-09
- Keywords:
- Qinghai-Tibet Plateau; artificial grass planting land; soil enzyme activity; soil enzyme stoichiometry
- 分类号:
- S154.2
- 文献标志码:
- A
- 摘要:
- [目的]探究青藏高原高寒草甸区不同人工牧草土壤酶活性及其化学计量特征,为深入认识高寒地区土壤养分状况及草地生态系统功能提供科学依据。[方法]以冷地早熟禾(Poa crymophila)、无芒披碱草(Elymus submuticus)、短芒披碱草(Elymus breviaristatus)、垂穗披碱草(Elymus nutans)、星星草(Puccinellia tenuiflora)、同德老芒麦(Elymus sibiricus)、贫花鹅观草(Roegneria pauciflora)7种人工牧草种植地土壤为研究对象,并以邻近的天然温带草原土壤作为对照(CK),探讨与土壤碳、氮和磷循环有关酶活性及其化学计量学特征。[结果](1)不同牧草种植地土壤酶活性差异较大〔(3～496 nmol/(g·h)〕,7种牧草土壤酶的活性均明显低于CK〔13～506 nmol/(g·h)〕。(2)土壤碳氮酶活性比(EC:N)在各样地之间无显著差异,冷地早熟禾样地的碳磷酶活性比(EC:P)和氮磷酶活性比(EN:P)最大(0.82, 0.94); 7种牧草土壤酶活性矢量角度均大于45°,表明牧区土壤微生物受到P元素限制。(3)土壤酶活性及其化学计量主要受土壤SOC,TN,W,MBC和MBN的影响,与MBC:MBN,SOC:TP和TN:TP也显著相关。[结论]人工牧草的种植改变了土壤养分结构从而影响土壤酶的分泌,不同牧草种类种植地土壤酶活性及其化学计量存在显著差异。未来结合土壤微生物群落组成以及功能特征可进一步揭示青藏高原高寒草甸土壤养分循环及草地生态系统功能的影响机制。
- Abstract:
- [Objective]The aims of this study are to explore the characteristics of soil enzyme activity and its chemical stoichiometry in alpine meadows of the Qinghai-Tibet Plateau under different artificial grass species, and provide scientific basis for a deeper understanding of soil nutrient status and grassland ecosystem function in the alpine pastoral areas. [Methods]Soils in seven different artificial grass planting including Poa crymophila, Elymus submuticus, Elymus breviaristatus, Elymus nutans, Puccinellia tenuiflora, Elymus sibiricus, and Roegneria pauciflora, were chosen as research objects, and the adjacent natural temperate grassland was used as a control(CK). The enzyme activity and chemical stoichiometry related to soil carbon, nitrogen and phosphorus cycles were investigated. [Results](1)The soil enzyme activity in different pasture planting sites was significantly different, which was in the range of 3～496 nmol/(g·h), and the soil enzyme activities of the seven pasture species were significantly lower than that of the control at 13～506 nmol/(g·h).(2)No significant difference in soil carbon/nitrogen enzyme ratio(EC:N)was observed among the different sites, and the carbon/phosphorus enzyme ratio(EC:P)at 0.82 and nitrogen/phosphorus enzyme ratio(EN:P)at 0.94 were the highest in the plot of Poa crymophila. The vector angles of soil enzyme activities for all seven grass types were greater than 45°, indicating that soil microorganisms in the pastoral areas were limited by phosphorus.(3)Soil enzyme activities and its chemical stoichiometry were mainly influenced by soil organic carbon, total nitrogen, soil moisture, microbial biomass carbon, and microbial biomass nitrogen, were also significantly related to MBC:MBN, SOC:TP and TN:TP. [Conclusion]The cultivation of artificial pasture has changed the nutrient structure of soil and affected the secretion of soil enzymes. There are significant differences in soil enzyme activity and chemical stoichiometry among different pasture species planting sites. In the future, the composition and functional characteristics of soil microbial communities can be further revealed to elucidate the mechanisms of nutrient cycling and grassland ecosystem function in high-altitude alpine meadow soils of the Qinghai-Tibet Plateau.
参考文献/References:
[1]龙瑞军.青藏高原草地生态系统之服务功能[J].科技导报,2007,25(9):26-28.
Long R J. Functions of ecosystem in the Tibetan grassland[J]. Science & Technology Review, 2007,25(9):26-28.
[2]刘兴元,冯琦胜.藏北高寒草地生态系统服务价值评估[J].环境科学学报,2012,32(12):3152-3160.
Liu X Y, Feng Q S. Evaluation of ecological services value of alpine rangeland ecosystem in the northern Tibet Region[J]. Acta Scientiae Circumstantiae, 2012,32(12):3152-3160.
[3]Sinsabaugh R L, Lauber C L, Weintraub M N, et al. Stoichiometry of soil enzyme activity at global scale[J]. Ecology Letters, 2008,11(11):1252-1264.
[4]Liu J B, Chen J, Chen G S, et al. Enzyme stoichiometry indicates the variation of microbial nutrient requirements at different soil depths in subtropical forests[J]. PLoS One, 2020,15(2):e0220599.
[5]Burns R G, DeForest J L, Marxsen J, et al. Soil enzymes in a changing environment:Current knowledge and future directions[J]. Soil Biology and Biochemistry, 2013,58:216-234.
[6]Peng X Q, Wang W. Stoichiometry of soil extracellular enzyme activity along a climatic transect in temperate grasslands of Northern China[J]. Soil Biology and Biochemistry, 2016,98:74-84.
[7]Sinsabaugh R L, Hill B H, Follstad Shah J J. Ecoenzymatic stoichiometry of microbial organic nutrient acquisition in soil and sediment[J]. Nature, 2009,462(7274):795-798.
[8]黄伟佳,刘春,刘岳,等.南岭山地不同海拔土壤生态化学计量特征及影响因素[J].生态环境学报,2023,32(1):80-89.
Huang W J, Liu C, Liu Y, et al. Soil ecological stoichiometry and its influencing factors at different elevations in Nanling Mountains[J]. Ecology and Environmental Sciences, 2023,32(1):80-89.
[9]郭超,盛茂银,何宇,等.土地利用方式对西南喀斯特土壤碳、氮、磷化学计量特征及酶活性的影响[J].土壤通报,2023,54(2):382-391.
Guo C, Sheng M Y, He Y, et al. Effects of land use types on soil carbon, nitrogen, phosphorus stoichiometric characteristics and enzyme activities in the karst area of southwest China[J]. Chinese Journal of Soil Science, 2023,54(2):382-391.
[10]Ma L N, Huang W W, Guo C Y, et al. Soil microbial properties and plant growth responses to carbon and water addition in a temperate steppe:The importance of nutrient availability[J]. PLoS One, 2012,7(4):e35165.
[11]欧阳克蕙,王堃.人工草地植被重建对退化红壤化学性质和酶活性的影响[J].江西农业大学学报,2007,29(3):474-478.
Ouyang K H, Wang K. Effects of artificial grassland reconstruction on soil nutrients and enzyme activity in degraded red soil[J]. Acta Agriculturae Universitatis Jiangxiensis, 2007,29(3):474-478.
[12]魏小星.青海湖东沙化治理过程中土壤酶活性及养分含量特征[J].草业科学,2017,34(7):1408-1418.
Wei X X. Soil nutrient content and the activities of soil enzymes during the desertification restoration process to the east of Qinghai Lake[J]. Pratacultural Science, 2017,34(7):1408-1418.
[13]Feyissa A, Gurmesa G A, Yang F, et al. Soil enzyme activity and stoichiometry in secondary grasslands along a climatic gradient of subtropical China[J]. The Science of the Total Environment, 2022,825:154019.
[14]Chen W J, Zhou H K, Wu Y, et al. Direct and indirect influences of long-term fertilization on microbial carbon and nitrogen cycles in an alpine grassland[J]. Soil Biology and Biochemistry, 2020,149:107922.
[15]German D P, Weintraub M N, Grandy A S, et al. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies[J]. Soil Biology and Biochemistry, 2011,43(7):1387-1397.
[16]Moorhead D L, Sinsabaugh R L, Hill B H, et al. Vector analysis of ecoenzyme activities reveal constraints on coupled C, N and P dynamics[J]. Soil Biology and Biochemistry, 2016,93:1-7.
[17]李小容,韦金玉,陈云,等.海南岛不同林龄的木麻黄林地土壤微生物的功能多样性[J].植物生态学报,2014,38(6):608-618.
Li X R, Wei J Y, Chen Y, et al. Functional diversity of soil microorganisms in Casuarina equisetifolia woodlands of different stand ages in Hainan Island[J]. Chinese Journal of Plant Ecology, 2014,38(6):608-618.
[18]赵海燕,徐福利,王渭玲,等.秦岭地区华北落叶松人工林地土壤养分和酶活性变化[J].生态学报,2015,35(4):1086-1094.
Zhao H Y, Xu F L, Wang W L, et al. Soil nutrients and enzyme activities in Larix principis-rupprechtii plantations in the Qinling Mountains, China[J]. Acta Ecologica Sinica, 2015,35(4):1086-1094.
[19]张威,张明,白震,等.土壤中几丁质酶的研究进展[J].土壤通报,2007,38(3):569-575.
Zhang W, Zhang M, Bai Z, et al. Research advances in soil chitinase[J]. Chinese Journal of Soil Science, 2007,38(3):569-575.
[20]Von Sperber C, Kries H, Tamburini F, et al. The effect of phosphomonoesterases on the oxygen isotope composition of phosphate[J]. Geochimica et Cosmochimica Acta, 2014,125:519-527.
[21]Sinsabaugh R L, Moorhead D L. Resource allocation to extracellular enzyme production:A model for nitrogen and phosphorus control of litter decomposition[J]. Soil Biology and Biochemistry, 1994,26(10):1305-1311.
[22]Allison S D, Weintraub M N, Gartner T B, et al. Evolutionary-economic Principles as Regulators of Soil Enzyme Production and Ecosystem Function[M]// Soil Enzymology, Berlin, Heidelberg:Springer, 2010:229-243.
[23]Stark S, Männistö M K, Eskelinen A. Nutrient availability and pH jointly constrain microbial extracellular enzyme activities in nutrient-poor tundra soils[J]. Plant and Soil, 2014,383(1):373-385.
[24]Wei L, Razavi B S, Wang W Q, et al. Labile carbon matters more than temperature for enzyme activity in paddy soil[J]. Soil Biology and Biochemistry, 2019,135:134-143.
[25]Cui Y X, Fang L C, Guo X B, et al. Ecoenzymatic stoichiometry and microbial nutrient limitation in rhizosphere soil in the arid area of the northern Loess Plateau, China[J]. Soil Biology and Biochemistry, 2018,116:11-21.
[26]万忠梅,宋长春.土壤酶活性对生态环境的响应研究进展[J].土壤通报,2009,40(4):951-956.
Wan Z M, Song C C. Advance on response of soil enzyme activity to ecological environment[J]. Chinese Journal of Soil Science, 2009,40(4):951-956.
[27]闫钟清,齐玉春,李素俭,等.降水和氮沉降增加对草地土壤微生物与酶活性的影响研究进展[J].微生物学通报,2017,44(6):1481-1490.
Yan Z Q, Qi Y C, Li S J, et al. Soil microorganisms and enzyme activity of grassland ecosystem affected by changes in precipitation pattern and increase in nitrogen deposition:a review[J]. Microbiology China, 2017,44(6):1481-1490.
[28]王晓龙,胡锋,李辉信,等.红壤小流域不同土地利用方式对土壤微生物量碳氮的影响[J].农业环境科学学报,2006,25(1):143-147.
Wang X L, Hu F, Li H X, et al. Effects of different land used patterns on soil microbial biomass carbon and nitrogen in small red soil watershed[J]. Journal of Agro-Environment Science, 2006,25(1):143-147.
相似文献/References:
[1]吴成启,唐登勇.近50年来全球变暖背景下青藏高原气温变化特征[J].水土保持研究,2017,24(06):262.
WU Chengqi,Tang Deng Yong.Change of Temperature in The Tibetan Plateau in the Context of Global Warming in Recent 50 Years[J].Research of Soil and Water Conservation,2017,24(01):262.
[2]王丽娟,郭铌,杨扬.基于MODIS遥感资料估算高原地表潜热通量[J].水土保持研究,2017,24(03):119.
WANG Lijuan,GUO Ni,YANG Yang.Surface Latent Heat Estimated by Remote Sensing Over the Tibetan Plateau[J].Research of Soil and Water Conservation,2017,24(01):119.
[3]魏东岚,李永化.青藏高原东缘第四纪泥石流沉积物地球化学分析[J].水土保持研究,2012,19(06):292.
WEI Dong-lan,LI Yong-hua.The Geochemistry Analysis of the Debris Flow Sediment in the Quaternary at the Eastern Fringe of Qinghai-Tibetan Plateau[J].Research of Soil and Water Conservation,2012,19(01):292.
[4]李 焱,戴 睿,张云霞,等.藏西南高原植被NDVI时空变化及其与海拔梯度的关系[J].水土保持研究,2022,29(04):215.
LI Yan,DAI Rui,ZHANG Yunxia,et al.Spatiotemporal Variation of Vegetation NDVI and Its Relationship with Altitude Gradient in Southwest Tibet Plateau[J].Research of Soil and Water Conservation,2022,29(01):215.
[5]陈宗伟,江玉林,张洪江,等.青藏高原高速公路建设中弃土场土壤流失特性——以青海平阿高速公路为例[J].水土保持研究,2006,13(06):4.
CHEN Zong-wei,JIANG Yu-lin,ZHANG Hong-jiang,et al.The Properties of the Soil Loss in the Spoil Area During the Construction of Expressway in Tibetan Plateau——Example as the Ping’a Express in Qinghai Province[J].Research of Soil and Water Conservation,2006,13(01):4.
[6]安志山,张克存,屈建军,等.青藏铁路沿线风沙灾害特点及成因分析[J].水土保持研究,2014,21(02):285.
AN Zhi-shan,ZHANG Ke-cun,QU Jian-jun,et al.Sand Hazard Characteristics and Genetic Analysis along the Qinghai-Tibet Railway[J].Research of Soil and Water Conservation,2014,21(01):285.
[7]郭小伟,杜岩功,李以康,等.高寒草甸植被层对于草地甲烷通量的影响[J].水土保持研究,2015,22(01):146.
GUO Xiaowei,DU Yangong,LI Yikang,et al.Effects of Vegetation Layers on Methane Flux in Alpine Meadow on the Tibetan Plateau[J].Research of Soil and Water Conservation,2015,22(01):146.
[8]周夏飞,於方,曹国志,等.2001-2015年青藏高原草地碳源/汇时空变化及其与气候因子的关系[J].水土保持研究,2019,26(01):76.
ZHOU Xiafei,YU Fang,CAO Guozhi,et al.Spatiotemporal Features of Carbon Source-Sink and Its Relationship with Climate Factors in Qinghai-Tibet Plateau Grassland Ecosystem During 2001-2015[J].Research of Soil and Water Conservation,2019,26(01):76.
[9]孟梦,牛铮,马超,等.青藏高原NDVI变化趋势及其对气候的响应[J].水土保持研究,2018,25(03):360.
MENG Meng,NIU Zheng,MA Chao,et al.Variation Trend of NDVI and Response to Climate Change in Tibetan Plateau[J].Research of Soil and Water Conservation,2018,25(01):360.
[10]王丽,张镱锂,王兆锋,等.放牧及水文扰动对拉萨地区湿草甸湿地土壤特征的影响[J].水土保持研究,2013,20(01):66.
WANG Li,ZHANG Yi-li,WANG Zhao-feng,et al.Effects of Grazing and Hydrological Disturbance on Soil Properties of Wet Meadow Wetland in Lhasa[J].Research of Soil and Water Conservation,2013,20(01):66.
备注/Memo
收稿日期:2024-04-29 修回日期:2024-05-07
资助项目:国家自然科学基金(42171301); 陕西省创新人才推进计划科技创新团队项目(2023-CX-TD-37)
第一作者:徐嘉翊(1997—),女,山东潍坊人,硕士研究生,研究方向为土壤微生物学。E-mail:xjyszbd328@163.com
通信作者:薛萐(1978—),男,陕西西安人,博士,研究员,主要从事土壤微生物和生态恢复研究。E-mail:xuesha100@163.com