PDF Download

[1]LIU Gengyuan,XIAO Jie,GAO Mingxia,et al.Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N2O Emission in Eum-Orthic Anthrosols[J].Research of Soil and Water Conservation,2022,29(02):99-105.

Copy

Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N₂O Emission in Eum-Orthic Anthrosols

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 29 Number of periods: 2022 02 Page number: 99-105 Column: Public date: 2022-03-20

Title:: Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N₂O Emission in Eum-Orthic Anthrosols

Author(s):: LIU Gengyuan¹, XIAO Jie¹, GAO Mingxia², SUN Benhua^1,4, ZHANG Shulan¹, YANG Xueyun¹, FENG Hao^2,3,4, ZHANG Tongxun¹; (1.College of Natural Resources and Environment, Northwest A&F University/Key Laboratory of Plant Nutrition and Agro-Environment, Ministry of Agriculture, Yangling,Shaanxi712100,China; 2.College of Water Resources and Architectural Engineering, Northwest A&F University, Yangling, Shaanxi712100,China; 3.Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi712100,China; 4.Institute of Water Saving Agriculture in Arid Region of China, Yangling, Shaanxi712100,China)

Keywords:: long-term fertilization; nirS-type denitrifier; nitrous oxide; quantitative real-time PCR; high-throughput sequencing

CLC:: X144; X172

DOI:: -

Abstract:: Soil denitrification is an important process for the production of soil N₂O, and that Nitrite reductase(NIR)catalyzes the reduction of nitrite nitrogen(NO^-₂)to nitric oxide(NO)is the key step of denitrification. The study on the impact of long-term fertilization on denitrifying microorganisms and its relationship with soil N₂O emissions is of great significance for a comprehensive understanding of the soil denitrification process. Based on 28 years of long-term fertilization experiments in rain-fed dry farming system, the characteristics of soil nirS-type denitrifying bacterial communities and N₂O emission and their relationship were investigated under different types of long-term fertilization(No fertilization of CK, only N fertilization and NK combined fertilization of partial fertilizations, and balanced fertilization of NPK)by conventional monitoring, quantitative PCR and high-throughput sequencing. The results showed that all types of long-term fertilization(N, NK and NPK)significantly increased the cumulative N₂O emission(EN₂O), in which balanced fertilization(NPK)caused the the highest cumulative N₂O emission; long-term fertilization had no significant effect on nirS gene abundance and the α-diversity of nirS-type denitrifying bacteria, while long-term balanced fertilization increased the relative abundance of uncultured_bacterium_2303 and Rhodanobacter_sp._D206a, and reduced the relative abundance of unclassified_k_norank_d_Bacteria and unclassified_p_Proteobacteria, thereby changed the community structure of nirS-type denitrifying bacteria. Soil properties such as soil organic carbon, total nitrogen, available phosphorusand pH are the main factors influencing the nirS-type denitrifying bacterial community structure that has a significant effect on soil N₂O emission, while the abundance of nirS gene abundance and the diversity of nirS-type denitrifying bacteria have no significant effects in Eum-Orthic Anthrosols under rain-fed dry farming system.

References:: [1] Butterbach-Bahl K, Baggs E M, Dannenmann M, et al. Nitrous oxide emissions from soils:how well do we understand the processes and their controls[J].Philosophical Transactions of the Royal Society B: Biological Sciences,2013,368(1621).DOI:10.1098/rstb.2013.0122.
[2] Syakila A, Kroeze C. The global nitrous oxide budget revisited[J]. Greenhouse Gas Measurement and Management,2011,1(1):17-26.
[3] Shi Y L, Liu X R, Zhang Q W. Effects of combined biochar and organic fertilizer on nitrous oxide fluxes and the related nitrifier and denitrifier communities in a saline-alkali soil[J]. Science of the Total Environment,2019,686:199-211.
[4] Zhou S, Huang T, Zhang C, et al. Illumina MiSeq sequencing reveals the community composition of NirS-Type and NirK-Type denitrifiers in Zhoucun reservoir: A large shallow eutrophic reservoir in northern China[J]. Rsc. Advances,2016,6(94):91517-91528.
[5] Tao R, Wakelin S A, Liang Y, et al. Nitrous oxide emission and denitrifier communities in drip-irrigated calcareous soil as affected by chemical and organic fertilizers[J]. Science of the Total Environment,2018,612:739-749.
[6] Heylen K, Gevers D, Vanparys B, et al.The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers[J]. Environmental Microbiology,2006,8(11):2012-2021.
[7] Dandie C E, Sophie W, Leclair C L, et al. Abundance, diversity and functional gene expression of denitrifier communities in adjacent riparian and agricultural zones[J]. FEMS Microbiol Ecol., 2011,77(1):69-82.
[8] Palmer K, Biasi C, Horn M A. Contrasting denitrifier communities relate to contrasting N₂O emission patterns from acidic peat soils in arctic tundra[J]. The Isme Journal, 2012,6(5):1058-1077.
[9] Braker G, Conrad R. Diversity, structure, and size of N₂O-producing microbial communities in soils:what matters for their functioning[J].Adv. Appl. Microbiol, 2011,75:33-70.
[10] Liu X, Chen C R, Wang W J, et al. Soil environmental factors rather than denitrification gene abundance control N₂O fluxes in a wet sclerophyll forest with different burning frequency[J]. Soil Biology and Biochemistry,2013,57:292-300.
[11] Barrett M, Khalil M I, Jahangir M M R, et al. Carbon amendment and soil depth affect the distribution and abundance of denitrifiers in agricultural soils[J]. Environmental Science and Pollution Research,2016,23(8):7899-7910.
[12] 程效义,刘晓琳,孟军,等.生物炭对棕壤NH₃挥发、N₂O排放及氮肥利用效率的影响[J].农业环境科学学报,2016,35(4):801-807.
[13] Huang R, Wang Y, Gao X, et al. Nitrous oxide emission and the related denitrifier community:A short-term response to organic manure substituting chemical fertilizer[J]. Ecotoxicology and Environmental Safety,2020,192.DOI:10.1016/j.ecoenv.2020.110291.
[14] Wang Q, Liu Y R, Zhang C, et al. Responses of soil nitrous oxide production and abundances and composition of associated microbial communities to nitrogen and water amendment[J]. Biology and Fertility of Soils,2017,53(6):601-611.
[15] Hu X, Liu J, Wei D, et al. Chronic effects of different fertilization regimes on nirS-type denitrifier communities across the black soil region of Northeast China[J]. Pedosphere, 2020,30(1):73-86.
[16] Sun R, Guo X, Wang D, et al. Effects of long-term application of chemical and organic fertilizers on the abundance of microbial communities involved in the nitrogen cycle[J]. Applied Soil Ecology, 2015,95:171-178.
[17] Yang X Y, Li P R, Zhang S L, et al. Long-term-fertilization effects on soil organic carbon, physical properties, and wheat yield of a loess soil[J]. Journal of Plant Nutrition and Soil Science, 2011,174(5):775-784.
[18] 吴娟,齐雁冰,常庆瑞,等.关中地区土系统分类归属及代表土系建立[J].土壤学报,2021,58(2):357-371.
[19] 肖杰,刘平静,孙本华,等.长期施用化肥对旱作雨养农田N₂O排放特征的影响[J].西北农林科技大学学报:自然科学版,2020,48(5):108-114,122.
[20] 郭慧楠,马丽娟,黄志杰,等.咸水滴灌对棉田土壤N₂O排放和反硝化细菌群落结构的影响[J].环境科学,2020,41(5):2455-2467.
[21] 郭芸,孙本华,王颖,等.长期施用不同肥料土PLFA指纹特征[J].中国农业科学,2017,50(1):94-103.
[22] 鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.
[23] 刘平静,肖杰,孙本华,等.长期不同施肥措施下土菌群落结构变化及其主要影响因素[J].植物营养与肥料学报,2020,26(2):307-315.
[24] Michotey V, Méjean V, Bonin P. Comparison of methods for quantification of cytochrome cd1-denitrifying bacteria in environmental marine samples[J]. Applied and Environmental Microbiology, 2000,66(4):1564-1571.
[25] Throbäck I N, Enwall K, Jarvis Å, et al. Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE[J]. FEMS Microbiol Ecol, 2004,49(3):401-417.
[26] Huse S M, Huber J A, Morrison H G, et al. Accuracy and quality of massively parallel DNA pyrosequencing[J]. Genome Biology, 2007,8(7):1-9.
[27] Quast C, Pruesse E, Yilmaz P, et al. The SILVA ribosomal RNA gene database project:Improved data processing and web-based tools[J]. Nucleic Acids Research, 2012,41:590-596.
[28] Fracetto F J C, Fracetto G G M, Bertini S C B, et al. Effect of agricultural management on N₂O emissions in the Brazilian sugarcane yield[J]. Soil Biology and Biochemistry, 2017,109:205-213.
[29] Morales S E, Jha N, Saggar S. Biogeography and biophysicochemical traits link N₂O emissions, N₂O emission potential and microbial communities across New Zealand pasture soils[J]. Soil Biology and Biochemistry, 2015,82:87-98.
[30] Francis C, O'Mullan G, Cornwell J, et al. Transitions in nirS-type denitrifier diversity, community composition, and biogeochemical activity along the Chesapeake Bay estuary[J]. Frontiers in Microbiology,2013,4.DOI:10.3389/fmicb.2013.00237.
[31] Bian R X, Sun Y J, Li W H, et al. Co-composting of municipal solid waste mixed with matured sewage sludge:The relationship between N₂O emissions and denitrifying gene abundance[J]. Chemosphere, 2017,189:581-589.
[32] Cui P Y, Fan F L, Yin C, et al. Long-term organic and inorganic fertilization alters temperature sensitivity of potential N₂O emissions and associated microbes[J]. Soil Biology and Biochemistry,2016,93:131-141.
[33] Liu C X, Zhao X R, Lin Q M, et al. Decrease in diversity and shift in composition of the soil bacterial community were closely related to high available phosphorus in agricultural Fluvisols of North China[J]. Acta Agriculturae Scandinavica Section B: Soil and Plant Science,2019,69(7):618-630.
[34] Fang S, Nan H, Lv D Q, et al. Effects of sulfoxaflor on greenhouse vegetable soil N₂O emissions and its microbial driving mechanism[J]. Chemosphere, 2021,267.DOI:10.1016/j.chemosphere.2020.129248.
[35] 曹文超,宋贺,王娅静,等.农田土壤N₂O排放的关键过程及影响因素[J].植物营养与肥料学报,2019,25(10):1781-1798.
[36] 胡晓婧,刘俊杰,于镇华,等.东北黑土nirS型反硝化细菌群落和网络结构对长期施用化肥的响应[J].植物营养与肥料学报,2020,26(1):1-9.
[37] Yin C, Fan F, Song A, et al. Denitrification potential under different fertilization regimes is closely coupled with changes in the denitrifying community in a black soil[J]. Applied Microbiology and Biotechnology, 2015,99(13):5719-5729.

Similar References:

Memo

Last Update: 2022-04-20