[1]Wang Huiling,Ma Huizhen,Wang Guoliang.Effects of different root exudate components on soil microorganisms and carbon and nitrogen mineralization in Pinus tabuliformis forests[J].Research of Soil and Water Conservation,2025,32(06):113-122.[doi:10.13869/j.cnki.rswc.2025.06.022]
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Effects of different root exudate components on soil microorganisms and carbon and nitrogen mineralization in Pinus tabuliformis forests
Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume:
32
Number of periods:
2025 06
Page number:
113-122
Column:
Public date:
2025-10-20
- Title:
- Effects of different root exudate components on soil microorganisms and carbon and nitrogen mineralization in Pinus tabuliformis forests
- Keywords:
- root exudates; soil nutrients; enzyme activity; soil organic carbon mineralization; nitrogen mineralization
- CLC:
- S154.2
- DOI:
- 10.13869/j.cnki.rswc.2025.06.022
- Abstract:
- [Objective] This study aims to explore the effects of carbohydrates, organic acids, and phenolic acids in root exudates, and their combined impact on soil microorganisms and carbon (C) and nitrogen (N) mineralization in forests, thereby deepening the understanding of rhizosphere processes. [Methods] Soil from artificial Pinus tabuliformis forests in loess hilly regions was taken as the research object. Soil samples were collected and laboratory incubation experiments were conducted with eight treatments of exudates： sterile distilled water(CK), glucose(G), oxalic acid(A), p-hydroxybenzoic acid(H), glucose+oxalic acid(GA), glucose+ p-hydroxybenzoic acid (GH), oxalic acid+p-hydroxybenzoic acid (AH), and glucose+oxalic acid+phydroxybenzoic acid(GAH). The changes in soil nutrients, microbial biomass, enzyme activities, and carbon and nitrogen mineralization rates were measured during continuous exudate addition over 84 days. [Results](1) The concentrations of available phosphorus (AP), nitrate nitrogen (NO3- -N), ammonium nitrogen (NH+4 -N), and microbial biomass were increased by all treatments of exudate addition. The promoting effects of A and H were generally stronger than those of G. The mixed addition of exudates had a greater effect on NH+4 -N, AP, and microbial biomass carbon (MBC) compared with the addition of single components. (2) Exudate addition increased soil enzyme activities within 0~56 days, and the promoting effect diminished or even shifted to inhibition by 84 days. During 0~28 days, G showed stronger promoting effects on Beta-Glucosidase, Cellobiohydrolase (CBH), and N-Acetyl-D-Glucosaminidase activities than A and H, but its effect gradually decreased, while the effects of A and H increased. Compared with single addition treatments, mixed addition of exudates did not significantly enhance enzyme activity and even reduced it.(3) Exudates addition during 0~14 days increased soil nitrification and ammonification rates. Specifically, the highest nitrification rates were found in the A, H, and GAH treatments, while the highest ammonification rates were found in the G, AH, and GAH treatments. Microbial biomass phosphorus (MBP), CBH, and AP were the most important factors influencing soil nitrification and ammonification. (4) The addition of each exudate increased soil organic carbon (SOC) mineralization, with greater promoting effects observed over the incubation periods. The cumulative CO2 emissions were highest in the GH and AH treatments, increasing by 42.8% and 40.8%, respectively, compared to CK. MBP, NO3- -N, NH+4 -N, and CBH were identified as the most important factors influencing CO2 emission. [Conclusion] The addition of exudates primarily enhances soil enzyme activity and nitrogen mineralization during the early stage, and intensify carbon mineralization during the later stage. Oxalic acid, p-hydroxybenzoic acid, and their mixed addition demonstrate greater efficacy than glucose in improving soil available nutrients and microbial biomass, thereby promoting soil carbon and nitrogen cycling processes.
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Last Update:
2025-12-10