[1]Ding Li,JI Yuliang,LI Yi.Soil Microbial Diversity and Its Influencing Factors in Rhizosphere and Non-Rhizosphere in the Stands of Pinus tabuliformis with Different Ages in Minjiang River Valley[J].Research of Soil and Water Conservation,2020,27(04):184-191,200.
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Soil Microbial Diversity and Its Influencing Factors in Rhizosphere and Non-Rhizosphere in the Stands of Pinus tabuliformis with Different Ages in Minjiang River Valley
Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume:
27
Number of periods:
2020 04
Page number:
184-191,200
Column:
目次
Public date:
2020-06-20
- Title:
- Soil Microbial Diversity and Its Influencing Factors in Rhizosphere and Non-Rhizosphere in the Stands of Pinus tabuliformis with Different Ages in Minjiang River Valley
- CLC:
- S154.3
- DOI:
- -
- Abstract:
- Microbial diversity and its influencing factors in rhizosphere and non-rhizosphere in the stands of Pinus tabulaeformis with different ages were studied by using Illumina-MiSeq high-throughput sequencing technologies to measure sequences of 16srDNA gene segments of V3—V4 area of bacteria and 18srDNA gene segments of V4 area of fungi, in order to provide useful management for Pinus tabulaeformis plantation. The results showed that:(1)pH value of the rhizosphere soil value of Pinus tabulaeformis was significantly lower than that of the non-rhizosphere(p<0.05), soil organic carbon, total nitrogen, available nitrogen and available phosphorus in rhizosphere were significantly higher than those of the non-rhizosphere(p<0.05), soil organic carbon, total nitrogen, available phosphorus and available potassium contents in rhizosphere and non-rhizosphere showed the consistent change trend, reaching to the maximum values in the mature forest;(2)activity of soil invertase, alkaline phosphatase, urease and catalase in rhizosphere were significantly higher than the non-rhizosphere, soil enzyme activities in rhizosphere and non-rhizosphere showed the consistent change trend, reaching to the maximum values in the mature forest and decreasing in the aged forest;(3)the diversity indexes of bacteria and fungi in the rhizosphere of Pinus tabulaeformis was higher than those in the non-rhizosphere; the richness index, shannon-wiener index, ACE index and Chao 1 index in rhizosphere were significantly higher than those in the non-rhizosphere with the growth of the age of Pinus tabulaeformis; however, there were no significant differences in the coverage, uniformity and Simpson index between the rhizosphere and the non-rhizosphere soil (p>0.05);(4)PCoA analysis showed that the bacterial and fungal communities in rhizosphere and non-rhizosphere of stands of Pinus tabulaeformis had the higher similarity, and the communities in rhizosphere and non-rhizosphere bacterial produced obvious separation effects;(5)redundancy analysis showed that the nine soil environmental factors explained the total characteristic values of 86% bacteria and 82% fungi, respectively, indicating that the soil environmental factors had a significant impact on the diversity of bacteria and fungi in soils of stands of Pinus tabulaeformis, soil organic carbon and total nitrogen had a great impact on soil bacterial and fungal diversity, while pH value had the negative impact on soil bacterial and fungal diversity.
- References:
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Last Update:
2020-07-09