[1]YANG Bingxun,LIU Quan,DONG Tingxu.Soil Bacterial Communities of Grasslands with Different Desertification Degrees in Northwest Sichuan[J].Research of Soil and Water Conservation,2018,25(06):45-52.
Copy
Soil Bacterial Communities of Grasslands with Different Desertification Degrees in Northwest Sichuan
Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume:
25
Number of periods:
2018 06
Page number:
45-52
Column:
Public date:
2018-10-26
- Title:
- Soil Bacterial Communities of Grasslands with Different Desertification Degrees in Northwest Sichuan
- CLC:
- Q948;S151
- DOI:
- -
- Abstract:
- To elucidate soil bacterial communities, especially in desertification grasslands, we explored soil bacterial communities by the method of high throughput sequencing technique (MiSeq). The sequence region was 16 SrRNA V3—V4 variable region. α diversity, community composition and the relative abundance of soil bacterial groups were analyzed in order to explore the effects of soil nutrients on bacterial community structure in different desertification grasslands including non-desertification grassland, light-desertification grassland, middle-desertification grassland and heavy-desertification grassland in northwest Sichuan. The results showed that: (1) there was a large difference in soil nutrients in different desertification grasslands, with the desertification in grassland, soil pH increased, while soil organic carbon, total nitrogen, total potassium, alkali-nitrogen and available phosphorus gradually decreased; (2) the structure and diversity of the microbial communities differed from different desertification grasslands; at the phylum level, the dominant phyla were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes and Firmicutes, at the class level, α-Proteobacteria Actinobacteria, Acidobacteria, β-Proteobacteria, Thermoleophilia, Gemmatimonadetes, Bacilli, δ-Proteobacteria were predominant; compared with desertification grasslands, the dominant phyla in non-desertification grassland was Proteobacteria and Actinobacteria; (3) with the increase of the desertification degree of grassland, OUTs, Chao, Ace and Shannon gradually decreased, while there were no significant difference in coverage and Simpson index; (4) the results from the canonical correspondence and Pearson correlation analysis showed that soil organic carbon and total nitrogen were the main factors that significantly affected the soil bacterial community composition and alpha diversity in desertification grasslands in northwest Sichuan.
- References:
-
[1] Simon J, Dannenmann M, Pena R, et al. Nitrogen nutrition of beech forests in a changing climate:importance of plant-soil-microbe water, carbon, and nitrogen interactions[J]. Plant and Soil, 2017,418(1/2):89-114.
[2] Chen Z, Wei K, Chen L, et al. Effects of the consecutive cultivation and periodic residue incorporation of Bacillus thuringiensis (Bt) cotton on soil microbe-mediated enzymatic properties[J]. Agriculture, Ecosystems & Environment, 2017,239:154-160.
[3] Hartmann M, Frey B, Mayer J, et al. Distinct soil microbial diversity under long-term organic and conventional farming[J]. Isme Journal, 2015,9(5):1177-1194.
[4] Carini P, Marsden P J, Leff J W, et al. Relic DNA is abundant in soil and obscures estimates of soil microbial diversity[J]. Nature Microbiology, 2017,2(3):16242.
[5] Maestre F T, Delgado-Baquerizo M, Jeffries T C, et al. Increasing aridity reduces soil microbial diversity and abundance in global drylands[J]. Proceedings of the National Academy of Sciences, 2015,112(51):15684-15689.
[6] Tedersoo L. Correspondence:Analytical flaws in a continental-scale forest soil microbial diversity study[J]. Nature Communications, 2017,8:15572.
[7] Zhalnina K, Dias R, de Quadros P D, et al. Soil pH determines microbial diversity and composition in the park grass experiment[J]. Microbial Ecology, 2015,69(2):395-406.
[8] Lange M, Eisenhauer N, Sierra C A, et al. Plant diversity increases soil microbial activity and soil carbon storage[J]. Nature Communications, 2015,6:6707.
[9] Leff J W, Jones S E, Prober S M, et al. Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe[J]. Proceedings of the National Academy of Sciences, 2015,112(35):10967-10972.
[10] 王岩春,干友民,费道平,等.川西北退牧还草工程区围栏草地植被恢复效果的研究[J].草业科学,2008,25(10):15-19.
[11] 石福孙, 吴宁, 罗鹏. 川西北亚高山草甸植物群落结构及生物量对温度升高的响应[J]. 生态学报, 2008, 28(11):5286-5293.
[12] 刘长秀,张宏,泽柏.灌丛对川西北高寒草甸土壤资源的影响[J].山地学报,2006,24(3):357-365.
[13] 鲍士旦.土壤农化分析[M].北京:科学出版社,2006.
[14] Steinauer K, Tilman D, Wragg P D, et al. Plant diversity effects on soil microbial functions and enzymes are stronger than warming in a grassland experiment[J]. Ecology, 2015,96(1):99-112.
[15] Tardy V, Spor A, Mathieu O, et al. Shifts in microbial diversity through land use intensity as drivers of carbon mineralization in soil[J]. Soil Biology and Biochemistry, 2015,90:204-213.
[16] McDaniel M D, Tiemann L K, Grandy A S. Does agricultural crop diversity enhance soil microbial biomass and organic matter dynamics? A meta-analysis[J]. Ecological Applications, 2014,24(3):560-570.
[17] Chu H, Sun H, Tripathi B M, et al. Bacterial community dissimilarity between the surface and subsurface soils equals horizontal differences over several kilometers in the western Tibetan Plateau[J]. Environmental Microbiology, 2016,18(5):1523-1533.
[18] Yuan Y, Si G, Wang J, et al. Bacterial community in alpine grasslands along an altitudinal gradient on the Tibetan Plateau[J]. Fems Microbiology Ecology, 2014,87(1):121-132.
[19] Zhang X F, Zhao L, Xu S J, et al. Soil moisture effect on bacterial and fungal community in Beilu River(Tibetan Plateau)permafrost soils with different vegetation types[J]. Journal of Applied Microbiology, 2013,114(4):1054-1065.
[20] Yergeau E, Hogues H, Whyte L G, et al. The functional potential of high Arctic permafrost revealed by metagenomic sequencing, qPCR and microarray analyses[J]. Isme Journal, 2010,4(9):1206.
[21] Zhalnina K, Dias R, de Quadros P D, et al. Soil pH determines microbial diversity and composition in the park grass experiment[J]. Microbial Ecology, 2015,69(2):395-406.
[22] Wang J T, Cao P, Hu H W, et al. Altitudinal distribution patterns of soil bacterial and archaeal communities along Mt. Shegyla on the Tibetan Plateau[J]. Microbial Ecology, 2015,69(1):135-145.
[23] Jeanbille M, Buée M, Bach C, et al. Soil parameters drive the structure, diversity and metabolic potentials of the bacterial communities across temperate beech forest soil sequences[J]. Microbial Ecology, 2016,71(2):482-493.
[24] Rousk J, B, Brookes P C, et al. Soil bacterial and fungal communities across a pHgradient in an arable soil[J]. Isme Journal, 2010,4(10):1340.
[25] Fierer N, Jackson R B. The diversity and biogeography of soil bacterial communities[J]. Proceedings of the National Academy of Sciences of the United States of America, 2006,103(3):626-631.
[26] Shen C, Xiong J, Zhang H, et al. Soil pH drives the spatial distribution of bacterial communities along elevation on Changbai Mountain[J]. Soil Biology and Biochemistry, 2013,57:204-211.
[27] Leff J W, Jones S E, Prober S M, et al. Consistent responses of soil microbial communities to elevated nutrient inputs in grasslands across the globe[J]. Proceedings of the National Academy of Sciences, 2015,112(35):10967-10972.
[28] Ansola G, Arroyo P, de Miera L E S. Characterisation of the soil bacterial community structure and composition of natural and constructed wetlands[J]. Science of the Total Environment, 2014,473:63-71.
[29] Gao Y, Wang J, Guo S, et al. Effects of salinization and crude oil contamination on soil bacterial community structure in the Yellow River Delta region, China[J]. Applied Soil Ecology, 2015,86:165-173.
- Similar References:
Memo
-
Last Update:
1900-01-01