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[1]DAI Di,ZI Hongbiao,YANG Youfang,et al.Responses of Soil Microbial Functional Diversity to Nitrogen Addition in an Alpine Meadow on Northwestern Plateau of Sichuan Province[J].Research of Soil and Water Conservation,2018,25(02):137-144.

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Responses of Soil Microbial Functional Diversity to Nitrogen Addition in an Alpine Meadow on Northwestern Plateau of Sichuan Province

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 25 Number of periods: 2018 02 Page number: 137-144 Column: Public date: 2018-04-03

Title:: Responses of Soil Microbial Functional Diversity to Nitrogen Addition in an Alpine Meadow on Northwestern Plateau of Sichuan Province

Author(s):: DAI Di, ZI Hongbiao, YANG Youfang, ADE Luji, CHEN Yan, LUO Xueping, WANG Changting; College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China

Keywords:: alpine meadow; microbial community diversity; N fertilizer; Biolog-ECO; fertilization gradient

CLC:: S812.2

DOI:: -

Abstract:: Biolog-Eco microplate is used in the study to reveal the effects of N addition with four rates of 0 g/m² (CK), 10 g/m² (N₁₀), 20 g/m² (N₂₀), 30 g/m² (N₃₀) on soil chemical properties and microbial functional diversity. The results showed that the N₂₀ and N₃₀ significantly increased available phosphorus, available nitrogen and ratio of root to soil, but decreased soil moisture content (SMC). Average well color development (AWCD) value decreased in surface soil layer (0-10 cm), but AWCD increased in deep soil layer (10-20 cm), especially under the treatment of N₂₀ N fertilizer reduced the Shannon-Wiener index, Pielou index and McIntosh index in the surface layer (0-10 cm), but it was opposite to that in the deep soil layer (10-20 cm). The principal component analysis showed that N fertilizer changed the metabolic functional type of soil microorganism and that carbohydrates, amino acid and acids were the main carbon sources utilized by microorganism. Redundancy analysis indicated that soil microbial functional diversity was mainly influenced by total nitrogen and SMC in 0-10 cm soil layer, but in the deep soil layer it was significant affected by SMC and AN. In summary, the addition of N fertilizer increased soil available nutrients, so, soil microbial functional diversity, capacity and quantity of carbon sources utilization were altered.

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