[1]WANG Bo,LI Kai-rong,CUI Bi-xiao,et al.Reponse of Anti-oxidation Protective System of Robinia pseudoacacia Seedlings to Petroleum Contamination[J].Research of Soil and Water Conservation,2014,21(02):251-256.
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Reponse of Anti-oxidation Protective System of Robinia pseudoacacia Seedlings to Petroleum Contamination
Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume:
21
Number of periods:
2014 02
Page number:
251-256
Column:
Public date:
2014-04-28
- Title:
- Reponse of Anti-oxidation Protective System of Robinia pseudoacacia Seedlings to Petroleum Contamination
- Keywords:
- R.pseudoacacia seedings; anti-oxidation protective system; petroleum contamination; phytoremediation
- CLC:
- S718.43;X503.23
- DOI:
- -
- Abstract:
- In order to investigate the self-adjusting adaptability mechanism of the anti-oxidation protective system of Robinia pseudoacacia seedings under petroleum contamination stress, the dynamic changes of anti-oxidation enzymes, antioxidants and the contents of hydrogenperoxide H2O2 and malondialdehyde MDA of one year old Robinia pseudoacacia seedlings in pot experiment were measured. The results showed that except ascrodate peroxidase (APX) activity, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and glutathione (GSH) content of Robinia pseudoacacia seedings were significantly lower than the control under the stress of 5~20 g/kg petroleum contamination for 30 days. The contents of ascorbicacid (AsA) and carotenoid (Car) remained stable at the same conditions and they became significantly higher than CK after stressed by 20 g/kg petroleum contamination for 30 days. Along with the increase of petroleum concentration and the stress duration (60~90 d), SOD and CAT activities and AsA and GSH contents generally became higher or equal to control level, while APX and GR activities and Car content decreased significantly over the increase of petroleum concentration. After stressed by petroleum contamination, H2O2 contents of seedings significantly increased while MDA contents were equal to or lower than that of control. The destruction of anti-oxidation protective system of Robinia pseudoacacia seedings of petroleum contamination below 20 g/kg concentration was not obvious. Antioxidants content maintenance and the synergistic effects of anti-oxidation enzymes and antioxidants could be obsverved as the main mechanisms under short- and long-term of petroleum contamination stress, respectively.
- References:
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