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[1]XIAO Ye,HUANG Zhigang,LI Youfeng,et al.Soil Microbial Community Structure and Diversity of Typical Vegetation Types in Chishui River Basin[J].Research of Soil and Water Conservation,2022,29(06):275-283.

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Soil Microbial Community Structure and Diversity of Typical Vegetation Types in Chishui River Basin

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 29 Number of periods: 2022 06 Page number: 275-283 Column: Public date: 2022-10-20

Title:: Soil Microbial Community Structure and Diversity of Typical Vegetation Types in Chishui River Basin

Author(s):: XIAO Ye¹, HUANG Zhigang², LI Youfeng³, ZHANG Yuguang⁴, WANG Mi⁵; (1.Gollege of Resources and Environment, Zunyi Normal University, Zunyi, Guizhou 563006, China; 2.Gollege of Biology and Agriculture, Zunyi Normal University, Zunyi, Guizhou 563006, China; 3.College of Chemistry and Chemical Engineering, Zunyi Normal University, Zunyi, Guizhou 563006, China; 4.Key Laboratory of Biodiversity Conservation of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, CAF, Beijing 100091, China; 5.Guizhou……)

Keywords:: Chishui River Basin; vegetation type; microbial community; high-throughput sequencing; environmental factors

CLC:: S154.37

DOI:: -

Abstract:: Soil microorganism is an important indicator of soil environmental quality. The characteristics and dominant species of soil microbial community under typical vegetation types in the Chishui River Basin were clarified in order to provide a theoretical basis for ecosystem restoration and management. In this study, high-throughput sequencing technology was used to determine the microbial community structure and diversity of soil in five typical vegetation types such as shrub(SH), mixed conifer broadleaf forest(MCBF), evergreen broad-leaved forest(EBF), Chinese fir forest(CFF)and bamboo forest(BF)and to investigate the main influencing factors. The results showed that:(1)There was no significant difference in the Chao1 index of bacteria and fungi among the different vegetation types, indicating that there was no difference in the total number of species observed among five vegetation types. The Shannon index showed that there were certain differences in microbial diversity among the five vegetation types. BF had the lowest bacterial diversity, which was significantly lower than that of SH and MCBF(p<0.05). The fungal diversity in SH and CFF was significantly higher than that in the other three planting types(p<0.05).(2)The dominant group of bacteria phylum(relative abundance>10%)in five vegetation types were Proteobacteria, Actinobacteriota and Acidobacteriota. Proteobacteria was dominant in SH, MCBF and EBF(the relative abundance ranged from 29.70% to 33.62%). But Actinobacteriota was the most abundant in CFF and BF, accounting for 32.88% and 29.88%, respectively. Ascomycota was the absolute dominant group of soil fungi in different vegetation types(with the highest relative abundance>49%).(3)The dominant genus in soil bacteria and fungi of five vegetation types were significantly different. At the genus level of bacteria, Vicinamibacterales and Bacillus were dominant in MCBF and BF, respectively. While Arthrobacter was the most dominant species in the other three vegetation types. At the fungal level, the dominant genus in SH and EBF was unclassified_p_Ascomycota. Mortierella was the most abundant taxa in CFF, while Saitozyma in MCBF and BF had the highest relative abundance.(4)NMDS analysis showed that there was significant differences in the spatial distribution of soil bacterial and fungal communities among different vegetation types.(5)Redundancy analysis showed that soil moisture content, pH value and TN had significant effects on soil bacterial community structure, while fungal community structure was significantly driven by pH, bulk density, TOC, TN and TP. Comprehensive analysis showed that MCNF had rich soil bacterial community and the growth of soil fungi in CFF was vigorous. While soil bacterial and fungal diversity were high in SH. It is necessary to take effective measures to improve soil nutrients of the main stand, to stimulate the growth of microorganisms and improve the soil environment.

References:: [1] 翟辉,张海,张超,等.黄土峁状丘陵区不同类型林分土壤微生物功能多样性[J].林业科学,2016,52(12):84-91.
[2] Wu L H, Liu J L, Zhao J. Pyrosequencing: based assessment of bacterial community structure in Wuliangsuhai Wetland[J]. Advanced Materials Research, 2014,955/959(30):459-462.
[3] 赵辉,周运超,任启飞.不同林龄马尾松人工林土壤微生物群落结构和功能多样性演变[J].土壤学报,2020,57(1):227-238.
[4] 丁令智,满秀玲,肖瑞晗,等.寒温带森林根际土壤微生物量碳氮含量生长季内动态变化[J].林业科学,2019,55(7):178-186.
[5] 杨云礼,徐明,邹晓,等.不同植被类型对黔中山地丘陵区土壤细菌群落特征的影响研究[J].生态与农村环境学报,2021,37(4):518-525.
[6] 夏北威.植被对土壤微生物群落结构的影响[J].应用生态学报,1998,9(3):296-300.
[7] Zhao C, Long J, Liao H, et al. Dynamics of soil microbial communities following vegetation succession in a karst mountain ecosystem, Southwest China[J]. Scientific reports, 2019,9(1):1-10.
[8] 张胜男,闫德仁,黄海广,等.短期封育对科尔沁沙地榆树疏林土壤微生物群落结构的影响[J].生态学杂志,2020,39(9):2860-2867.
[9] Nottingham A T, Noah F, Turner B L, et al. Microbes follow humboldt: temperature drives plant and soil microbial diversity patterns from the amazon to the andes[J]. Ecology, 2018,99(11):2455-2466.
[10] 刘洋,黄懿梅,曾全超.黄土高原不同植被类型下土壤细菌群落特征研究[J].环境科学,2016,37(10):3931-3938.
[11] 赵静,唐剑波,黄尚书,等.赤水河流域水土流失类型区划分及防治对策[J].湖北农业科学,2015,54(14):3369-3371.
[12] 鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.
[13] 李金业,陈庆锋,李青,等.黄河三角洲滨海湿地微生物多样性及其驱动因子研究[J].生态学报,2021,41(15):1-10.
[14] 汪思龙,黄志群,王清奎,等.凋落物的树种多样性与杉木人工林土壤生态功能[J].生态学报,2005,25(3):474-480.
[15] Koga K, Suehiro Y, Matsuoka S T, et al. Evaluation of growth activity of microbes in tea field soil using microbial calorimetry[J]. Journal of Bioscience and Bioengineering, 2003,95(5):429-434.
[16] Yao S R, Merwin I A, Bird G W, et al. Orchard floor management practices that maintain vegetative or biomass groundcover stimulate soil microbial activity and alter soil microbial community composition[J]. Plant and Soil, 2005,271(1/2):377-389.
[17] Yang J K, Zhang J J, Yu H Y, et al. Community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees[J]. Applied Microbiology and Biotechnology, 2014,98:1449-1458.
[18] Ramirez K S, Craine J M, Fierer N. Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes [J]. Global Change Biology, 2012,18(6):1918-1927.
[19] Griffiths R I, Thomson B C, James P, et al. The bacterial biogeography of British soils [J]. Environmental Microbiology, 2011,13:1642-1654.
[20] 范雅倩,安菁,梁晨.北京市松山国家级自然保护区典型植被群落的土壤微生物群落结构特征[J].北方园艺,2021(1):81-86.
[21] Ferreira de Araujo A S, Bezerra W M, Santos V M, et al. Fungal diversity in soils across a gradient of preserved Brazilian Cerrado[J]. Journal of Microbiology, 2017,55(4):273-279.
[22] Crowther T W, Van Den Hoogen J, Wan J, et al. The global soil community and its influence on biogeoche-mistry[J]. Science, 2019,365(6455).DOI:10.1126/science.aav0550.
[23] 孙英杰,何成新,徐广平,等.广西十万大山地区不同植被类型土壤微生物特征[J].生态学杂志,2015,34(2):352-359.
[24] 费裕翀,叶义全,郑宏,等.外源氮素调控C/N比对杉木林凋落叶细菌群落结构的影响[J].生态学报,2021,41(5):2011-2023.
[25] Yang Y, Dou Y X, Huang Y M, et al. Links between soil fungal diversity and plant and soil properties on the Loess Plateau[J]. Frontiers in Microbiology, 2017,8.DOI:10.3389/fmicb.2017.02198.
[26] Zhang C, Wang J, Liu G, et al. Impact of soil leachate on microbial biomass and diversity affected by plant diversity[J]. Plant and Soil, 2019,439(1):505-523.
[27] He D, Shen W J, Eberwein J, et al. Diversity and co-occurrence network of soil fungi are more responsive than those of bacteria to shifts in precipitation seasonality in a subtropical forest[J]. Soil Biology and Biochemistry, 2017,115:499-510.
[28] 刘旻霞,蒋晓轩,李全弟,等.兰州北山不同植被土壤可培养微生物丰度变化特征及影响因素[J].中国环境科学,2020,40(6):2683-2691.

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Last Update: 2022-10-20