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[1]WANG Runchao,CHEN Jiarui,WANG Guoliang.Effects of Glucose Addition on Stoichiometric Characteristics of Microorganisms and Enzymes in Forest Soils at Different Succession Stages[J].Research of Soil and Water Conservation,2022,29(02):154-162.

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Effects of Glucose Addition on Stoichiometric Characteristics of Microorganisms and Enzymes in Forest Soils at Different Succession Stages

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 29 Number of periods: 2022 02 Page number: 154-162 Column: Public date: 2022-03-20

Title:: Effects of Glucose Addition on Stoichiometric Characteristics of Microorganisms and Enzymes in Forest Soils at Different Succession Stages

Author(s):: WANG Runchao^1,2, CHEN Jiarui^1,2, WANG Guoliang^1,2; (1.Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China; 2.Institute of Soil and Water Conservation, CAS & MWR, Yangling, Shaanxi 712100, China)

Keywords:: ecological stoichiometry; succession; root exudates; soil enzymes

CLC:: S718.5

DOI:: -

Abstract:: As an important root exudate, how sugars affect the stoichiometric characteristics of soil microorganisms and enzymes is not clear, which restricts understanding of the above process. In order to explore the effects of sugars on soil microecological environment and enzyme activity in forest ecosystem, the pioneer(poplar forest), middle stage(Pinus tabulaeformis forest)and climatic top community(Liaodong oak forest)of forest in Ziwuling area of Loess Plateau were selected as the sampling sites. The effects of glucose addition on soil microbial biomass, enzyme activity and stoichiometry were studied by adding three different concentrations of glucose(0, 0.1, 0.5, 1 g/kg dry soil)to soils sampled in the forests and indoor culture methods. The results showed that:(1)with forest succession, soil available nutrients such as available phosphorus(AP)and nitrate nitrogen(NO^-₃-N)decreased significantly; under the influence of glucose, the soil TN of three kinds of woodland decreased, and the soil C/N value of poplar forest was significantly higher than that of Pinus tabulaeformis forest and Liaodong oak forest;(2)with forest succession, the values of microbial carbon(MBC)and microbial nitrogen(MBN)in Populus davidiana forest and Liaodong oak forest were significantly higher than those in Pinus tabulaeformis forest, which decreased at first and then increased; with the increase of glucose concentration, MBC and MBN in three kinds of woodland increased, and MBP showed a fluctuating trend;(3)the soil enzyme activities of Populus davidiana forest and Liaodong oak forest were significantly higher than those of Pinus tabulaeformis forest; with the increase of glucose concentration, the activities of four enzymes in Pinus tabulaeformis forest and Liaodong oak forest increased at first and then decreased; the values of BG/(LAP+NAG), BG/AP and(LAP+NAG)/AP of Liaodong oak forest were the highest, and these values of Populus davidiana forest and Liaodong oak forest were significantly higher than those of Pinus tabulaeformis forest;(4)RDA analysis showed that there was a significant positive correlation between soil enzyme, microbial biomass and enzyme activity and SOC, MBC, respectively, and a significant negative correlation between soil enzyme activity(except NAG)and pH. This study showed that carbon input increased the stimulating effect of three kinds of forest soil, increased soil microbial biomass, decreased soil TN content, changed soil nutrient stoichiometry, and then intensified microbial N limitation in Pinus tabulaeformis woodland and Liaodong microbial P limitation in oak woodland, affected the decomposition of organic matter and nutrient metabolism in rhizosphere soil, thus affected the process of forest succession.

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Last Update: 2022-04-20