[1]SUN Qiang,WANG Li-yan,JING Rui-yong,et al.Tolerant Capacity of Drought Stress and Response Mechanism of Protective Enzyme of Different Ploidy Isatis indigotica[J].Research of Soil and Water Conservation,2013,20(06):48-53.
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Tolerant Capacity of Drought Stress and Response Mechanism of Protective Enzyme of Different Ploidy Isatis indigotica
Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume:
20
Number of periods:
2013 06
Page number:
48-53
Column:
Public date:
2013-12-28
- Title:
- Tolerant Capacity of Drought Stress and Response Mechanism of Protective Enzyme of Different Ploidy Isatis indigotica
- Keywords:
- diploid; tetraploid; Isatis indigotica; drought stress; protective enzyme
- CLC:
- S567.239
- DOI:
- -
- Abstract:
- To compare tolerant capacity to drought stress of Isatis indigotica with different ploidy and response mechanism of protective enzyme in vivo, different concentration mannitol was added into MS medium to imitate salt stress. For diploid and tetraploid of Isatis indigotica, germination of seed and seeding growth condition were investigated, and malondialdehyde, proline contents in seedling and protective enzyme in overground part including SOD, POD and CAT were measured. The results showed that tolerant stress of tetraploid of Isatis indigotica was better than that of diploid by germination, seedling growth and malondialdehyde and proline contents. The results showed that SOD, POD and CAT activities of tetraploid and diploid appeared the trend of increase-decrease with increase of concentration of mannitol by protective enzyme. SOD, POD and CAT activities of tetraploid were higher than those of diploid under different concentrations of mannitol. The results mentioned above showed that the ability of removing active oxygen for tetraploid was higher than that of diploid under salt stress, which can partly explain that toleranct ability to salt stress of tetraploid was higher that of diploid.
- References:
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