PDF Download

[1]WANG Li-yan,JING Rui-yong,GUO Yong-xia,et al.Resistant Ability of Isatis indigotica Seedling with Different Ploid to Salting and Responding Mechanism of Its Protective Enzyme[J].Research of Soil and Water Conservation,2013,20(04):224-229.

Copy

Resistant Ability of Isatis indigotica Seedling with Different Ploid to Salting and Responding Mechanism of Its Protective Enzyme

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 20 Number of periods: 2013 04 Page number: 224-229 Column: Public date: 2013-08-28

Title:: Resistant Ability of Isatis indigotica Seedling with Different Ploid to Salting and Responding Mechanism of Its Protective Enzyme

Author(s):: WANG Li-yan, JING Rui-yong, GUO Yong-xia, YIN Kui-de; Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China

Keywords:: diploid; tetraploid; Isatis indigotica; salt stress; protective enzyme

CLC:: Q945.78

DOI:: -

Abstract:: To compare tolerant capacity of different ploidy Isatis indigotica to salt stress and response mechanism of protective enzymes in vivo, different concentrations of sodium chloride were added into MS medium to imitate salt stress. Germination of seed and seeding growth condition of Diploid and tetraploid of Isatis indigotica were measured, malondialdehyde and proline content in seedling were measured and protective enzymes in aboveground part including SOD, POD and CAT were deter mined. The results of germination, seedling growth and malondialdehyde and proline content showed that tolerant stress of tetraploid of Isatis indigotica is better than that of diploid. Results of protective enzyme showed that enzyme which plays primary role is different, It was showed that active oxygen removal ability of tetraploid is higher than that of diploid under salt stress that SOD, POD and CAT activity of tetraploid were higher than those of diploid regardless of salt stress and sodium chloride, which can partly explain that tolerant ability of tetraploid to salt stress is higher than that of diploid.

References:: [1] 中华人民共和国药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2010:191.
[2] 乔传卓,戴富宝,崔熙,等.两种倍性水平鞍蓝的生药学研究[J].中草药,1995,26(8):423-428.
[3] 陈桂平,客绍英.二倍体及四倍体菘蓝氨基酸含量的测定分析[J].中药材,2011,34(4):506-508.
[4] 段英姿.菘蓝二倍体及其同源四倍体遗传差异的ISSR分析[J].西北植物学报,2012,32(8):1534-4538.
[5] 陈玉芹,聂姬锋,客绍英,等.不同倍性菘蓝超氧化物歧化酶的活性变化[J].唐山师范学院学报,2008,30(2):44-47.
[6] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:258.
[7] 高俊凤.植物生理学实验技术[M].西安:世界图书出版公司,2000:194.
[8] 梁新华,史大刚.干旱胁迫对光果甘草幼苗根系丙二醛含量及保护酶POD、CAT活性的影响[J].干旱地区农业研究,2006,24(3):108-110.
[9] 谭勇,梁宗锁,安玉艳.冬季干旱胁迫下黄土高原三种常绿树种叶片渗透调节物质变化研究[J].水土保持研究,2007,14(3):70-73.
[10] 付艳,高树仁,杨克军,等.盐胁迫对玉米耐盐系与盐敏感系苗期几个生理生化指标的影响[J].植物生理学报,2011,47(5):459-462.
[11] 张春平,何平,袁凤刚,等.外源5-氨基乙酰丙酸对干旱胁迫下甘草种子萌发及幼苗生理特性的影响[J].西北植物学报,2011,31(8):1603-1610.
[12] Levitt J. Response of Plants to Environmental Stress[M]. New York:Academic Press,1980.
[13] 赵黎芳,张金政,张启翔,等.水分胁迫下扶芳藤幼苗保护酶活性和渗透调节物质的变化[J].植物研究,2003,23(4):437-441.
[14] 王建华,刘鸿先,徐同.超氧物歧化酶(SOD)在植物逆境和衰老生理中的作用[J].植物生理学通讯,1989(1):1-7.
[15] 段慧荣,李毅,马彦军.PEG胁迫对沙冬青种子萌发过程的影响[J].水土保持研究,2011,18(3):221-225.
[16] 崔大练,马玉心,石戈,等.紫穗槐幼苗叶片对不同干旱梯度胁迫的生理生态响应[J].水土保持研究,2010,17(2):178-182.
[17] 曹兵,宋丽华,唐春慧.臭椿幼苗对渗透胁迫的生理响应[J].水土保持研究,2008,15(4):168-170.
[18] Willekens H, Chamnongpol S, Davey M, et al. Catalase is a sink for H₂O₂ and is indispensable for stress defence in C₃ plants[J].The EMBO J ournal,1997,16(16):4806-4816.

Similar References:

Memo

Last Update: 1900-01-01