PDF Download

[1]AN Yu,SHEN Ying-bai,ZHANG Zhi-xiang.Effects of Damage Stress on Membrane Lipid Peroxidation and Antioxidant Enzymes in Poplar Leaves[J].Research of Soil and Water Conservation,2010,17(05):241-244.

Copy

Effects of Damage Stress on Membrane Lipid Peroxidation and Antioxidant Enzymes in Poplar Leaves

Research of Soil and Water Conservation[ISSN 1005-3409/CN 61-1272/P] Volume: 17 Number of periods: 2010 05 Page number: 241-244 Column: Public date: 2010-10-20

Title:: Effects of Damage Stress on Membrane Lipid Peroxidation and Antioxidant Enzymes in Poplar Leaves

Author(s):: AN Yu¹, SHEN Ying-bai², ZHANG Zhi-xiang²; 1. Institute of Desertification Control, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;
2. College of Biological Science and Biotechnology, Beijing Forestry University, Beijing 100083, China

Keywords:: damage stress; reactive oxygen species; antioxidant enzymes; insect resistance; poplar

CLC:: Q946

DOI:: -

Abstract:: The concentration of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were measured in poplar leaves damaged by mechanical damage and herbivore wounding. The results showed that the concentration of H₂O₂ and MDA and the activities of SOD, CAT and POD were enhanced; the change of membrane lipid peroxidation and antioxidant enzymes was significantly higher in the herbivore wounding treatment (except CAT). The results suggest that the concentration of H₂O₂ and MDA were closely related to the change in antioxidant enzymes activities. A high level of antioxidant enzymes could decrease membrane lipid peroxidation levels and effectively induce plant defense responses. Meanwhile, the difference between mechanical damage and herbivore wounding suggest that H₂O₂, SOD, CAT and POD are associated with insect resistance in poplar.

References:: [1] Baldwin I T.An ecologically motivated analysis of plant-herbivore interactions in native tobacco[J].Plant Physiol.,2001,127:1449-1458.
[2] León J,Rojo E,Sánchez-Serrano J J.Wound signaling in plants[J].J.Exp.Bot.,2001,52:1-9.
[3] Blokhina O,Virolainen E,Fagerstedt K V.Antioxidants,oxidative damage and oxygen deprivation stress[J].Ann.Bot.,2003,91:179-194.
[4] Mur L,Kenton P,Draper J.In planta measurements of oxidative bursts elicited by avirulent and virulent bacterial pathogens suggests that H₂O₂ is insufficient to elicit cell death in tobacco[J].Plant Cell Environ.,2005,28:548-561.
[5] S’lesak I,S’lesak H,Libik M,et al.Antioxidant response system in the short-term post-wounding effect in Mesembryanthemum crystallinum leaves[J].J.Plant Physiol.,2008,165:127-137.
[6] Maffei M E,Mithfer A,Arimura G,et al.Effects of feeding Spodoptera littoralis on lima bean leaves.Ⅲ:Membrane depolarization and involvement of hydrogen peroxide[J].Plant Physiol.,2006,140:1022-1035.
[7] Mithfer A,Wanner G,Boland W.Effects of feeding Spodoptera littoralis on lima bean leaves.Ⅱ.Continuous mechanical wounding resembling insect feeding is sufficient to elicit herbivory-related volatile emission[J].Plant Physiol.,2005,137:1160-1168.
[8] 刘俊,吕波,徐朗莱.植物叶片中过氧化氢含量测定方法的改进[J].生物化学与生物物理进展,2000,27(5):548-551.
[9] 赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210.
[10] 邹琦.植物生理生化试验指导[M].北京:中国农业出版社,1998:97-99.
[11] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:164-169.
[12] Fadzilla N M,Finch R P,Burdon R H.Salinity,oxidative stress and antioxidant responses in shoot culture of rice[J].J.Exp.Bot.,1997,48:325-331.
[13] Bandeoglu E,Eyidogan F,Yucel M,et al.Antioxidant responses of shoots and roots of lentil to NaCl-salinity stress[J].Plant Growth Regul.,2004,42:69-77.
[14] 陈少裕.膜质过氧化对植物细胞的伤害[J].植物生理学通讯,1991,27(2):84-90.
[15] Levine A,Tenhaken R,Dixon R,et al.H₂O₂ from the oxidative burst orchestrates the plant hypersensitive disease resistance response[J].Cell,1994,79:583-593.

Similar References:

Memo

Last Update: 1900-01-01