MOU Hongxia,ZHANG Wenwen,LIU Bingru.Soil Fungi Community Diversity in Medicago sativa Field of Different Planting Years at Yellow River Diversion Irrigation Area[J].Research of Soil and Water Conservation,2021,28(04):91-96.
引黄灌区不同种植年限紫花苜蓿土壤真菌群落多样性特征
- Title:
- Soil Fungi Community Diversity in Medicago sativa Field of Different Planting Years at Yellow River Diversion Irrigation Area
- 文章编号:
- 1005-3409(2021)04-0091-06
- Keywords:
- yellow river irrigation area; planting years; Medicago sativa; fungal communities; diversity
- 分类号:
- S154.39
- 文献标志码:
- A
- 摘要:
- 为了解引黄灌区紫花苜蓿土壤真菌结构及多样性特征随种植年限的演变规律与变化机理,为人工草地管理提供科学依据,采用18SrDNA高通量测序方法分析引黄灌区种植不同年限的紫花苜蓿(1～6 a)土壤真菌的物种群落组成和丰度、Alpha多样性及Beta多样性与菌群所具有的结构,同时对土壤真菌群落组成与土壤理化因子进行了相关性分析。结果表明:在属水平下,毛壳属、镰孢属、赤霉菌属、Lectera、小画线壳属为优势菌属; 多样性分析表明,种植时间为5 a时,土壤真菌菌群丰度和多样性均达到最高,种植时间为2 a与3 a时,物种多样性组成相似性达到最高; 随着种植年限的变化,土壤真菌群落变化表现为先减小后增大的变化趋势,且与土壤理化性质具有显著相关关系。pH值、速效氮和有机碳是影响紫花苜蓿土壤真菌群落组成的主要因子。
- Abstract:
- In order to understand the soil fungal structure and diversity characteristics of Medicago sativa in Yellow River diversion irrigation area, change mechanism of planting years were studied, and the scientific basis for artificial grassland management was provided. The 18SrDNA high—throughput sequencing method was used to analyze the community composition and abundance, Alpha diversity, Beta diversity and the structure of the bacterial community of Medicago sativa(1～6 a)planted in the Yellow River irrigation area for different years. The composition of the fungal community was correlated with the soil physical and chemical factors relevant analysis. The results showed that the dominant genere of fungi detected were Chaetomium, Fusarium, Gibberella, Lectera, Monograph Ella at the genus level. The results of diversity analysis showed that the abundance and diversity of soil fungal flora reached the highest when the planting time was 5 years, and the similarity of species diversity composition reached the highest when the planting time was 2 years and 3 years. With the change of planting years, the changes of soil fungal communities showed a trend of first decreasing and then increasing, and had a significant correlation with soil physical and chemical properties. The pH value, available nitrogen, and organic carbon are the main factors affecting the composition of Medicago sativa fungal community.
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备注/Memo
收稿日期:2020-08-27 修回日期:2020-09-21资助项目:国家自然科学基金地区项目“封育年限对宁夏荒漠草原植物—土壤—微生物C、N、P生态化学计量特征的影响”(31660168)第一作者:牟红霞(1996—),女,宁夏银川人,在读硕士,研究方向为土壤微生物多样性。E-mail:mou18309573621@163.com通信作者:刘秉儒(1972—),男,甘肃庆阳人,研究员,主要从事生态修复与生物多样性研究。E-mail:bingru.liu@163.com