YANG Bingxun,LIU Quan,DONG Tingxu.Soil Bacterial Communities of Grasslands with Different Desertification Degrees in Northwest Sichuan[J].Research of Soil and Water Conservation,2018,25(06):45-52.
川西北不同沙化程度草地土壤细菌群落特征
- Title:
- Soil Bacterial Communities of Grasslands with Different Desertification Degrees in Northwest Sichuan
- 分类号:
- Q948;S151
- 摘要:
- 研究川西北不同沙化程度草地（未沙化草地、轻度沙化草地、中度沙化草地、重度沙化草地）土壤细菌多样性和群落结构特征，利用Illumina二代高通量测序技术MiSeq对土壤细菌的16 SrRNA V3—V4可变区进行测序，研究土壤细菌多样性、物种组成和丰富度，并结合土壤理养分探讨影响细菌群落结构的环境因素，对发挥土壤潜在肥力，了解土壤健康状况，实现该区植被的管理与可持续利用有着重要的意义。结果表明：（1）不同沙化草地土壤养分具有明显差异，依次表现为：随着沙化程度的增加，土壤pH值逐渐增加，而土壤有机碳、全氮、全钾、碱解氮和速效磷逐渐降低；（2）不同沙化程度草地土壤样品中共检测到细菌的32个门，65个纲，169个目，优势菌门为变形菌门（Proteobacteria）、放线菌门（Actinobacteria）、酸杆菌门（Acidobacteria）、绿弯菌（Chloroflexi）、浮霉菌门（Planctomycetes），主要的优势菌纲为放线杆菌纲（Actinobacteria）、α-变形菌纲（α-Proteobacteria）、酸杆菌纲（Acidobacteria）、β-变形菌纲（β-Proteobacteria）、浮霉菌纲（Planctomycetacia），与沙化草地相比，未沙化草地优势菌主要是变形菌门（Proteobacteria）和放线菌门（Actinobacteria）；（3）随着沙化程度的增加，OUT数目、Chao指数、Ace指数、Shannon指数逐渐减小，其中不同沙化草地土壤细菌覆盖率和Simpson指数差异不显著（p > 0.05）；（4）冗余分析和Pearson相关性分析表明，土壤pH值、土壤有机碳（SOC）和全氮（TN）是土壤细菌群落结构和多样性的主要影响因子。
- Abstract:
- To elucidate soil bacterial communities, especially in desertification grasslands, we explored soil bacterial communities by the method of high throughput sequencing technique (MiSeq). The sequence region was 16 SrRNA V3—V4 variable region. α diversity, community composition and the relative abundance of soil bacterial groups were analyzed in order to explore the effects of soil nutrients on bacterial community structure in different desertification grasslands including non-desertification grassland, light-desertification grassland, middle-desertification grassland and heavy-desertification grassland in northwest Sichuan. The results showed that: (1) there was a large difference in soil nutrients in different desertification grasslands, with the desertification in grassland, soil pH increased, while soil organic carbon, total nitrogen, total potassium, alkali-nitrogen and available phosphorus gradually decreased; (2) the structure and diversity of the microbial communities differed from different desertification grasslands; at the phylum level, the dominant phyla were Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes and Firmicutes, at the class level, α-Proteobacteria Actinobacteria, Acidobacteria, β-Proteobacteria, Thermoleophilia, Gemmatimonadetes, Bacilli, δ-Proteobacteria were predominant; compared with desertification grasslands, the dominant phyla in non-desertification grassland was Proteobacteria and Actinobacteria; (3) with the increase of the desertification degree of grassland, OUTs, Chao, Ace and Shannon gradually decreased, while there were no significant difference in coverage and Simpson index; (4) the results from the canonical correspondence and Pearson correlation analysis showed that soil organic carbon and total nitrogen were the main factors that significantly affected the soil bacterial community composition and alpha diversity in desertification grasslands in northwest Sichuan.
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备注/Memo
收稿日期:2018-03-06;改回日期:2018-04-17。
基金项目:四川省教育厅项目：不同尺度条件下紫色土磷素迁移过程与效应（16ZB0311）
作者简介:杨秉珣(1975-),男,四川绵阳人,讲师,研究方向:生态环境与国土资源。E-mail:Bingxun_yang@163.com
通讯作者:董廷旭(1968-),男,四川苍溪人,本科,教授,研究方向:生态评价与GIS等。E-mail:yingxie1025@163.com