Ding Li,JI Yuliang,LI Yi.Soil Microbial Diversity and Its Influencing Factors in Rhizosphere and Non-Rhizosphere in the Stands of Pinus tabuliformis with Different Ages in Minjiang River Valley[J].Research of Soil and Water Conservation,2020,27(04):184-191,200.
不同林龄油松根际土壤微生物群落多样性及其影响因子
- Title:
- Soil Microbial Diversity and Its Influencing Factors in Rhizosphere and Non-Rhizosphere in the Stands of Pinus tabuliformis with Different Ages in Minjiang River Valley
- 文章编号:
- 1005-3409(2020)04-0184-08
- 分类号:
- S154.3
- 文献标志码:
- A
- 摘要:
- 为了探究不同林龄油松根际与非根际土壤微生物群落多样性及其与土壤环境因子之间的关系,利用Illumina-MiSeq高通量测序技术对不同林龄油松根际与非根际土壤中细菌的16SrDNA基因V3—V4区片段和真菌18SrDNA基因V4区片段进行测序,为油松的人工培育林提供有益借鉴和参考。结果表明:(1)油松根际土壤pH值显著低于非根际(p<0.05),根际土壤有机碳、全氮、有效氮和有效磷均显著高于非根际(p<0.05),土壤中根际和非根际有机碳、全氮、有效磷和有效钾含量呈一致的变化趋势,随着油松林龄的增长表现出先增加后降低趋势,在成熟林阶段达到最大值。(2)土壤中根际土壤酶活性(蔗糖酶活性、碱性磷酸酶活性、脲酶活性、过氧化氢酶活性)显著高于非根际,随着油松林龄的增长表现出先增加后降低趋势,在成熟林阶段达到最大值,过熟林有所降低。(3)油松根际土壤细菌和真菌多样性指数均高于非根际,其中随着油松林龄的增长表现出先增加后降低趋势,丰富度指数、Shannon-Wiener指数、ACE、Chao 1指数均显著高于非根际(p<0.05); 而根际土壤细菌和真菌覆盖度、均匀度和Simpson指数指数与非根际差异并不显著(p>0.05)。(4)主坐标分析(PCoA)分析表明,油松土壤根际和非根际细菌和真菌群落具有很好的相似性,并且根际和非根际细菌群落产生明显的分离效应。(5)冗余分析显示,土壤环境因子对油松土壤细菌和真菌多样性有显著影响。其中对土壤细菌和真菌多样性影响较大的有SOC和TN,而pH值对土壤细菌和真菌多样性影响为负。
- Abstract:
- Microbial diversity and its influencing factors in rhizosphere and non-rhizosphere in the stands of Pinus tabulaeformis with different ages were studied by using Illumina-MiSeq high-throughput sequencing technologies to measure sequences of 16srDNA gene segments of V3—V4 area of bacteria and 18srDNA gene segments of V4 area of fungi, in order to provide useful management for Pinus tabulaeformis plantation. The results showed that:(1)pH value of the rhizosphere soil value of Pinus tabulaeformis was significantly lower than that of the non-rhizosphere(p<0.05), soil organic carbon, total nitrogen, available nitrogen and available phosphorus in rhizosphere were significantly higher than those of the non-rhizosphere(p<0.05), soil organic carbon, total nitrogen, available phosphorus and available potassium contents in rhizosphere and non-rhizosphere showed the consistent change trend, reaching to the maximum values in the mature forest;(2)activity of soil invertase, alkaline phosphatase, urease and catalase in rhizosphere were significantly higher than the non-rhizosphere, soil enzyme activities in rhizosphere and non-rhizosphere showed the consistent change trend, reaching to the maximum values in the mature forest and decreasing in the aged forest;(3)the diversity indexes of bacteria and fungi in the rhizosphere of Pinus tabulaeformis was higher than those in the non-rhizosphere; the richness index, shannon-wiener index, ACE index and Chao 1 index in rhizosphere were significantly higher than those in the non-rhizosphere with the growth of the age of Pinus tabulaeformis; however, there were no significant differences in the coverage, uniformity and Simpson index between the rhizosphere and the non-rhizosphere soil (p>0.05);(4)PCoA analysis showed that the bacterial and fungal communities in rhizosphere and non-rhizosphere of stands of Pinus tabulaeformis had the higher similarity, and the communities in rhizosphere and non-rhizosphere bacterial produced obvious separation effects;(5)redundancy analysis showed that the nine soil environmental factors explained the total characteristic values of 86% bacteria and 82% fungi, respectively, indicating that the soil environmental factors had a significant impact on the diversity of bacteria and fungi in soils of stands of Pinus tabulaeformis, soil organic carbon and total nitrogen had a great impact on soil bacterial and fungal diversity, while pH value had the negative impact on soil bacterial and fungal diversity.
参考文献/References:
[1] 罗安程,章永松.有机肥对水稻根际土壤中微生物和酶活性的影响[J].植物营养与肥料学报,1999,5(4):321-327.
[2] 刘素慧,刘世琦,张自坤,等.大蒜连作对其根际土壤微生物和酶活性的影响[J].中国农业科学,2010,43(5):1000-1006.
[3] 安韶山,李国辉,陈利顶.宁南山区典型植物根际与非根际土壤微生物功能多样性[J].生态学报,2011,31(18):5225-5234.
[4] 吴林坤,黄伟民,王娟英,等.不同连作年限野生地黄根际土壤微生物群落多样性分析[J].作物学报,2015,41(2):308-317.
[5] 陈悦,吕光辉,李岩.独山子区优势草本植物根际与非根际土壤微生物功能多样性[J].生态学报,2018,38(9):3110-3117.
[6] 马海林,刘方春,马丙尧,等.保水剂对侧柏容器苗根际土壤微生物种群结构及干旱适应能力的影响[J].应用与环境生物学报,2016,22(1):43-48.
[7] 李艳春,李兆伟,林伟伟,等.施用生物质炭和羊粪对宿根连作茶园根际土壤微生物的影响[J].应用生态学报,2018,29(4):1273-1282.
[8] 郭小强,毛宁,张希彪.不同施肥处理对辣椒根际土壤微生物区系和酶活性的影响[J].作物杂志,2018,30(6):123-126.
[9] 董宇飞,吕相漳,张自坤,等.不同栽培模式对辣椒根际连作土壤微生物区系和酶活性的影响[J].浙江农业学报,2019,31(9):1485-1492.
[10] 周世雄,魏朝俊,胡海燕,等.氟磺胺草醚对大豆根际土壤微生物和酶活性的影响及其在根际的降解[J].植物营养与肥料学报,2018,24(1):203-211.
[11] 张雪梅,张秀梅,张沛东,等.固氮微生物对鳗草植株生长及其根际土壤酶活性的影响[J].中国海洋大学学报:自然科学版,2019,49(4):22-32.
[12] 井大炜,邢尚军,刘方春,等.保水剂施用方式对侧柏根际微生态环境的影响[J].农业机械学报,2016,47(5):146-154.
[13] 田秋阳,周鸿章,鲁萍.外来杂草反枝苋对大豆根际土壤微生物碳源利用和土壤理化性质的影响[J].作物杂志,2018,28(2):24-30.
[14] 李岩,何学敏,杨晓东,等.不同生境黑果枸杞根际与非根际土壤微生物群落多样性[J].生态学报,2018,38(17):5983-5995.
[15] 王继玥,刘燕,刘勇,等.基于高通量测序检测Pb污染对三叶草根际土壤细菌多样性的影响[J].环境科学研究,2018,31(1):102-110.
[16] 黄锦文,唐莉娜,曾文龙,等.不同土壤处理对烤烟根际土壤生物学特性的影响[J].中国烟草学报,2016,22(2):75-83.
[17] 张恩平,田悦悦,李猛,等.长期不同施肥对番茄根际土壤微生物功能多样性的影响[J].生态学报,2018,38(14):5027-5036.
[18] 安世花,罗安焕,王小利,等.长期施肥对黄壤旱地及水田土壤微生物生物量碳、氮的影响[J].河南农业科学,2019,48(6):73-80.
[19] 唐海明,李超,肖小平,等.有机肥氮投入比例对双季稻田根际土壤微生物生物量碳、氮和微生物熵的影响[J].应用生态学报,2019,30(4):1335-1343.
[20] 汪其同,高明宇,刘梦玲,等.基于高通量测序的杨树人工林根际土壤真菌群落结构[J].应用生态学报,2017,28(4):1177-1183.
[21] 曹永昌,杨瑞,刘帅,等.秦岭典型林分夏秋两季根际与非根际土壤微生物群落结构[J].生态学报,2017,37(5):1667-1676.
[22] 李越,曹瑾,汪春明,等.蚕豆间作栽培对连作马铃薯根际土壤微生物的影响[J].农业科学研究,2017,38(2):8-13.
[23] 戴雅婷,侯向阳,闫志坚,等.库布齐沙地两种植被恢复类型根际土壤微生物群落功能多样性研究[J].草业学报,2016,25(10):56-65.
[24] 唐海明,肖小平,李微艳,等.长期施肥对双季稻田根际土壤微生物群落功能多样性的影响[J].生态环境学报,2016,25(3):402-408.
[25] 武志海,刘晶晶,付丽,等.溶磷菌对大豆根际土壤酶活性及微生物群落的影响[J].中国农业大学学报,2017,22(11):58-67.
[26] 张奇,王海斌,李立,等.田间直播下化感水稻抑草作用及其根际土壤微生物生理特性动态[J].应用与环境生物学报,2018,5(3):8-12.
[27] 蒋岁寒,刘艳霞,孟琳,等.生物有机肥对烟草青枯病的田间防效及根际土壤微生物的影响[J].南京农业大学学报,2016,39(5):784-790.
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备注/Memo
收稿日期:2019-05-28 修回日期:2019-08-26
资助项目:重庆文理学院科学研究基金资助项目“永川种植业主产区耕地质量调查评价及质量提升对策”(R2019FLX19)
第一作者:丁丽(1985—),女,内蒙古赤峰人,硕士,讲师,主要从事生态环境保护研究。E-mail:dingli_85@163.com
通信作者:李懿(1965—),男,四川成都人,博士,副教授,主要从事微生物学、资源与环境微生物研究。E-mail:Leeyii_811@163.com