LAI Qiaohui,ZHANG Hao,LIU Zhipeng.Characteristics of Soil Microbial Community and Their Imapact Factors in Different Constructed Wetlands with Plant Communities[J].Research of Soil and Water Conservation,2019,26(05):89-94,99.
不同植物配置下人工湿地微生物群落特征及其影响因素
- Title:
- Characteristics of Soil Microbial Community and Their Imapact Factors in Different Constructed Wetlands with Plant Communities
- 分类号:
- X524
- 摘要:
- 植物是湿地生态系统的重要组分，本选择茭白（Zizania caduciflora）、鸢尾（Iris tectorum）、菖蒲（Acorus calamus）和芦苇（Phragmites australis），连续5年研究了不同植物配置下人工湿地微生物群落特征对环境的指示作用，结合微生物群落冗余分析（Redundancy analysis，RDA），研究了不同植物配置下人工湿地土壤环境因子及其对微生物群落分布的影响。结果表明：（1）土壤有机碳、全氮、全磷、全钾、速效磷和碱解氮呈一致的变化规律，均表现为芦苇 > 菖蒲 > 茭白 > 鸢尾，而不同人工湿地全磷差异不显著（p>0.05）。（2）人工湿地对土壤微生物量有较大的影响，土壤微生物量碳、氮、磷呈一致的变化规律，均表现为芦苇 > 菖蒲 > 茭白 > 鸢尾，不同人工湿地微生物量碳和氮差异均显著（p<0.05），微生物量磷差异不显著（p>0.05）。（3）物种丰富度指数（H）、均匀度指数（E）、和碳源利用丰富度指数（S）均表现为芦苇 > 菖蒲 > 茭白 > 鸢尾，而优势度指数（Ds）则表现为鸢尾 > 茭白 > 菖蒲 > 芦苇。其中不同人工湿地碳源利用丰富度指数（S）差异均显著（p<0.05），不同人工湿地优势度指数（Ds）差异均不显著（p>0.05）。（4）相关性分析表明不同人工湿地微生物物种丰富度指数（H）与SOC，TN呈显著正相关，芦苇和菖蒲的相关系数高于茭白和鸢尾，说明芦苇和菖蒲人工湿地微生物群落多样性高于茭白和鸢尾；RDA分析表明，土壤环境因子具有明显的生态梯度，SOC，TN是影响人工湿地微生物群落多样性的主要环境因子。
- Abstract:
- Hygrophytes are the important parts of a constructed wetland system. Four common wetland plants (Phragmites australis, Acorus calamus, Iris tectorum and Zizania caduciflora) in natural wetlands were chosen. The study compared the environmental indicator of soil microbial community in different constructed wetlands of plant communites by redundancy analysis during five years. The results showed that:(1) soil organic carbon, total nitrogen, total phosphorus, total potassium, available phosphorus and alkali-hydrolyzed nitrogen showed consistent change rules, which follow the sequence:Phragmites australis > Acorus calamus > Zizania caduciflora > Iris tectorum, but there was no significant difference in total phosphorus in different constructed wetlands; (2) constructed wetlands had great impact on soil microbial biomass, and the soil microbial biomass of carbon, nitrogen and phosphorus showed the consistent change rule, which follow the order:Phragmites australis > Acorus calamus > Zizania caduciflora > Iris tectorum; the differences of microbial biomass carbon and nitrogen of different constructed wetlands were significant (p<0.05), while the difference of of phosphorus microbial biomass was not significant (p>0.05); (3) species richness index (H), evenness index (E), and carbon source utilization richness index (S) decreased in the order:Phragmites australis > Acorus calamus > Iris tectorum > Zizania caduciflora, and dominance indexes (Ds) decreased in the order:Phragmites australis > Acorus calamus > Zizania caduciflora > Iris tectorum; the differences in carbon source utilization richness indexes (S) of different constructed wetlands were significant (p<0.05), while differences in Ds of different constructed wetlands were not significant (p>0.05); (4) correlation analysis indicated that the microbial species richness indexes of different constructed wetlands were significantly positively correlated with SOC and TN; The correlation coefficients of reed wetland and calamus wetland were higher than those of water shoot wetland and iris wetland, indicating that the microbial community diversities of constructed reed and calamus wetlands of were higher than those of water shoot and iris wetlands; RDA analysis showes that soil environmental factors have obvious ecological gradients, and SOC and TN are the main environmental factors affecting the microbial community diversities of constructed wetlands.
参考文献/References:
[1] 王雨轩,魏巍,李萍萍,等.人工湿地微生物的研究进展[J].生物技术通报,2017,33(10):74-79.
[2] 熊家晴,李珊珊,葛媛,等.处理高污染河水垂直流人工湿地微生物群落特性[J].环境工程学报,2017,11(3):1959-1965.
[3] 易乃康,彭开铭,陆丽君,等.人工湿地植物对脱氮微生物活性的影响机制研究进展[J].水处理技术,2016,42(4):12-16.
[4] 吕纯剑,高红杰,宋永会,等.潮汐流-潜流组合人工湿地微生物群落多样性研究[J].环境科学学报,2018,38(6):2140-2149.
[5] 吴文卫,刘昂,谷照虎,等.采用PCR-DGGE技术研究处理农田退水组合人工湿地微生物群落特征[J].应用与环境生物学报,2016,22(6):978-985.
[6] 王林,李冰,朱健.高通量测序技术在人工湿地微生物多样性研究中的研究进展[J].中国农学通报,2016,32(5):10-15.
[7] 陈亮,刘锋,肖润林,等.人工湿地氮去除关键功能微生物生态学研究进展[J].生态学报,2017,37(18):6265-6274.
[8] 李振灵,丁彦礼,白少元,等.潜流人工湿地基质结构与微生物群落特征的相关性[J].环境科学,2017,38(9):3713-3720.
[9] 房昀昊,彭剑峰,宋永会,等.高通量测序法表征潜流人工湿地中不同植物根际细菌群落特征[J].环境科学学报,2018,38(3):911-918.
[10] 王宁宁,赵阳国,孙文丽,等.溶解氧含量对人工湿地去除污染物效果的影响[J].中国海洋大学学报:自然科学版,2018,48(6):24-30.
[11] 刘佳,易乃康,熊永娇,等.人工湿地构型对水产养殖废水含氮污染物和抗生素去除影响[J].环境科学,2016,37(9):3430-3437.
[12] 唐美珍,李玉洁,张锦,等. Pseudomonas flava WD—3对人工湿地中土著微生物量及酶活性的影响研究[J].环境科学学报,2016,36(4):1236-1241.
[13] 袁敏,刘晓冰,唐美珍.生物炭固定菌强化人工湿地对低温污水中氮素去除的模拟研究[J].生态与农村环境学报,2018,34(5):463-468.
[14] 王林,李冰,余家辉,等.不同湿地模型中根系微生物的多样性[J].环境科学,2017,38(8):3312-3318.
[15] 周旭丹,孙晓刚,赵春莉,等.人工湿地植被根区土壤性质及其净化水质季节效应分析[J].生态环境学报,2015,24(6):1043-1049.
[16] 张盛博,何小娟,吴海露,等.环境因子对人工湿地沉积物中反硝化微生物群落结构的影响[J].水处理技术,2017,43(4):11-14.
[17] 林燕,张焕杰,刘曦,等.固定反硝化菌强化人工湿地处理低污染水研究[J].农业环境科学学报,2016,35(11):2154-2162.
[18] 程宪伟,梁银秀,祝惠,等.人工湿地处理水体中抗生素的研究进展[J].湿地科学,2017,15(1):125-131.
[19] 王苏艳,宋新山,赵志淼,等.亚铁对水平潜流人工湿地反硝化作用的影响[J].环境科学学报,2016,36(2):557-563.
[20] 常军军,吴苏青,梁康,等.复合垂直流人工湿地微生物特征对典型污水的响应差异[J].环境科学研究,2016,29(8):1200-1206.
[21] 周桑扬,杨凯,吴晓芙,等.人工湿地植物去除废水中重金属的作用机制研究进展[J].湿地科学,2016,14(5):717-724.
[22] 宋志文,王琳,徐爱玲,等.人工湿地空气微生物群落碳源代谢特征[J].环境科学,2015,36(2):415-420.
[23] 蒲艾平,刘晓玲,罗鸿兵,等.葡萄糖碳源对复合垂直流人工湿地甲烷排放的驱动影响[J].应用与环境生物学报,2017,23(4):719-727.
[24] 张骁栋,李伟,潘旭,等.表流人工湿地氮素形态组成及去除效率研究[J].生态环境学报,2016,25(3):503-509.
[25] 仇付国,徐艳秋,许俊挺,等.人工湿地系统除磷影响因素研究进展[J].科技导报,2017,35(9):23-29.
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[1]石岩,万新南.人工湿地系统在垃圾渗滤液处理中的应用[J].水土保持研究,2005,12(01):138.
SHI Yan,WAN Xin-nan.Application of Constructed Wetland to Landfill Leachate Treatment[J].Research of Soil and Water Conservation,2005,12(05):138.
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备注/Memo
收稿日期:2019-01-14;改回日期:2019-02-20。
基金项目:广东省教育厅“农林类职业教育绿色技能系统开发与研究”（GDJG2015100）
作者简介:赖巧晖(1983-),女,广东兴宁人,硕士,讲师,研究方向:植被生态学。E-mail:Qiao_huilai@126.com
通讯作者:张浩(1981-),男广东湛江人,副教授,研究方向:环境工程。E-mail:Qiao_huilai@126.com