LI Fengxia,ZHAO Ying.Effect of Nitrogen Fertilizer Reduction With Microbial Inoculants on Broccoli Production and the Influence of the Soil Microbial Characteristics[J].Research of Soil and Water Conservation,2017,24(02):94-100.
氮肥减量配施微生物菌剂对灌淤土花椰菜产量及土壤微生物的影响
- Title:
- Effect of Nitrogen Fertilizer Reduction With Microbial Inoculants on Broccoli Production and the Influence of the Soil Microbial Characteristics
- Keywords:
- broccoli; nitrogen fertilizer reduction; microbial inoculants; microbial diversity; microbial number
- 分类号:
- S154.2
- 摘要:
- 采用biolog微生物鉴定系统和平板稀释法探讨了氮肥减量配施自制微生物菌剂对花椰菜产量及根际土壤微生物的影响研究。结果表明：T4（2/3倍N肥用量+微生物菌剂）、T5（3/4倍N肥用量+微生物菌剂）和T6（常规N肥用量+微生物菌剂）处理土壤细菌、放线菌和微生物总数数量分别增加了57.6%~321.54%，66.81%~83.62%和135.40%~232.07%，而土壤真菌数量降低34.08%~41.80%。与对照相比各施肥处理增加了土壤细菌占微生物总数的比例，降低了真菌和放线菌的比例，T5和T4处理效果最显著。与对照相比T4，T5和T6处理显著提高了土壤微生物群总落碳源利用能力。T4，T5和T6处理显著增加了土壤微生物群落对碳水化合物、羧酸类、聚合物类和氨基酸类碳源的代谢水平，T3（单施微生物菌剂）处理显著增加了土壤微生物群落对羧酸类和聚合物类碳源的代谢水平。T5和T6处理对增加花椰菜产量、生物量效果接近，花椰菜产量分别增加了12.35%和10.75%，地下干生物量增加了57%左右，T2（单施氮肥）和T4处理增加花椰菜产量5%左右。
- Abstract:
- The effect of nitrogen fertilizer reduction with microbial inoculants on broccoli production and the impact of the soil microbial characteristics have been studied using biolog microbial identification system and plate dilution methods. The results indicate that the total numbers of soil bacteria, actinomyces and microbial quantity of T4(2/3 times of N fertilizer + microbial fertilizer treatment), T5 (3/4 times of N fertilizer + microbial fertilizer treatment)and T6 (normal amount of N fertilizer + microbial fertilizer treatment) treatments increase by 57.6%~321.54%, 66.81%~83.62% and 135.40%~232.07% and the number of soil fungi reduces by 34.0 841.80%. Compared with the control, all the fertilizer treatments increase the proportion of the total number of microorganisms of soil bacteria, while the proportion of the fungi and actinomycetes is reduced, the effects of T4 and T5 treatments are the most significant. T4, T5, and T6 treatments significantly increase the soil microbiota total carbon source utilization ability. T4, T5 and T6 treatments significantly improve the carbon source metabolism activity of soil microbial community on carbohydrate, acid, polymer and amino acids. T3(microbial fertilizer) treatment significantly increases the carbon source metabolism activity of soil microbial community on carboxylic acid and polymer. T5 and T6 treatments have the same effect on the effect of increasing broccoli production and biomass, the broccoli production increases by 12.35% and 10.75% respectively, underground stem biomass increases about 57%, T2 (normal amount of N fertilizer) and T4 treatments increased broccoli yields by about 5%.
参考文献/References:
[1] 张丽荣,陈杭,康萍芝,等.不同微生物菌剂对番茄产量及土壤微生物数量的影响[J].湖北农业科学,2013,52(22):5453-5456.
[2] 洪坚平,谢英荷, Neumann Guenter,等.两种微生物菌剂对小麦幼苗生长和磷吸收机理的影响研究[J].中国生态农业学报,2008,18(1):105-108.
[3] 杨玉新,王纯立,谢志刚,等.微生物肥对土壤微生物种群数量的影响[J].新疆农业科学,2008,45(1):169-171.
[4] 王涛,李剑,覃娟.几种微生物菌剂处理下连作黄瓜的生长分析[J].北方园艺,2010(18):15-19.
[5] 马锋敏,杨利民,肖春萍.施用微生物菌剂对参后地土壤微生物功能多样性的影响[J].吉林农业大学学报,2015,37(3):317-322.
[5] 朱英,孙权,司海丽,等.微生物菌剂对设施番茄幼苗生长的影响[J].北方园艺,2013(19):55-58.
[6] 千淋兆,龚明波,顾金刚,等.溶磷微生物菌剂对土壤营养元素及玉米生长的影响[J].农业资源与环境学报,2014,31(5):425-431.
[7] 高峰,尤垂淮,刘朝科,等.丁博锐,张重义.施用微生物菌剂对烤烟经济性状及其根际微生态变化的影响[J].福建农业学报,2014,29(12):1230-1235.
[9] 弓新国,胡晓峰,陈俊秋,等..不同时期施用微生物菌剂对烤烟生长和产量质量的影响[J].中国土壤与肥料,2014(6):106-110.
[10] 贺冰,赵月平,邵秀丽,等.微生物菌剂与化学肥料配施对番茄幼苗生长的影响[J].河南农业大学学报2010,44(5):528-531.
[11] 雷先德,李金文,徐秀玲,等.微生物菌剂对菠菜生长特性及土壤微生物多样性的影响[J].中国生态农业学报.2012,20(4):488-494.
[12] 李凤霞,王学琴,郭永忠,等.宁夏不同类型盐渍化土壤微生物区系及多样性[J].水土保持学报,2011,25(5):107-111.
[13] 中国科学院南京土壤研究所微生物室.土壤微生物研究法[M].北京:科学出版社,1985.
[14] 李凤霞,杨涓,许兴,等.脱硫废弃物施用对盆栽油葵碱化土壤微生物多样性的影响[J].土壤,2011,43(2):270-276.
[15] 李凤霞,王学琴,郭永忠,等.宁夏引黄不同盐化程度土壤酶活性及微生物多样性研究[J].水土保持研究,2013,20(1):61-65.
[16] 中国科学院南京土壤研究所.土壤理化分析[M].上海:上海科学技术出版社,1983.
[17] 田长平,高安妮,张福兴,孙庆.微生物菌剂对设施甜樱桃产量和品质的影响[J].烟台果树,2015,129(1),12-13.
[18] 谭兆赞,林捷,刘可星,廖宗文.复合微生物菌剂对番茄青枯病和土壤微生物多样性的影响.[J].华南农业大学学报,2007,28(1):45-49.
备注/Memo
收稿日期:2016-01-21;改回日期:2016-04-09。
基金项目:宁夏农林科学院自主研发项目（NKYG-15-15、NKYJ-16-03）；宁夏自然科学基金项目（NZ14189）；国家自然科学基金项目（41661066）
作者简介:李凤霞(1977-)女,宁夏固原人,博士,副研究员,主要从事农田生态与土壤微生物方面的研究工作。E-mail:lifengxia1211@sina.com
通讯作者:赵莹(1979-),男,河南项城人,博士,副研究员,主要从事土壤培肥与农田养分循环研究。E-mail:tony029@126.com