CUI Da-lian,MA Yu-xin,SHI Ge,et al.Ecophysiological Responses of Amorpha Fruticosa L. Seeding Leaves to Long-term Drought Gradient Treatment[J].Research of Soil and Water Conservation,2010,17(02):178-181,185.
紫穗槐幼苗叶片对不同干旱梯度胁迫的生理生态响应
- Title:
- Ecophysiological Responses of Amorpha Fruticosa L. Seeding Leaves to Long-term Drought Gradient Treatment
- Keywords:
- Amorpha Fruticosa L.; drought gradient; leaf; ecophysiology
- 分类号:
- S718.3
- 摘要:
- 研究了长期不同干旱梯度胁迫条件下紫穗槐幼苗叶片各种生理生化指标变化。结果表明：轻度、中度、重度胁迫条件下SOD、POD活性明显高于对照，而且随着胁迫时间的延长而逐渐增高。重度胁迫下SOD、POD活性与轻度、中度差异显著。胁迫前期（1~60 d）SOD、POD活性变化具有波动现象，胁迫后期（60~120 d）SOD活性下降，POD活性上升。轻度、中度、重度胁迫条件下可溶性蛋白质、脯氨酸含量高于对照，而且随着胁迫时间的延长而增高。重度胁迫下可溶性蛋白质、脯氨酸含量与轻度、中度差异显著，轻度、中度胁迫条件下可溶性蛋白质、脯氨酸含量差异不显著；从第80天始脯氨酸含量下降，而可溶性蛋白质含量增高。轻度、中度、重度胁迫条件下，可溶性糖含量差异较小。轻度、中度、重度胁迫条件下MDA含量、细胞膜相对透性高于对照，且逐渐增高，重度与轻度、中度差异显著，细胞膜透性变化与MDA含量变化成明显的正相关，MDA含量变化与SOD活性变化成正相关。
- Abstract:
- Changes in some physiological and biochemical indexes of A. fruiticosa L. seeding leaves under long-term different drought gradient treatment was studied by using pot culture 2-year-old A. fruiticosa seeding as experiment materials and adopting the method of weighing the soil to control the water content, four different levels of different drought gradient treatment. The water stresses were the control(CK), light stress(LS), medium stress(MS) and severe stress(SS). The results showed that superoxide dismutase (SOD), peroxidase (POD) activity of A. fruiticosa seedlings in LS, MS, SS were obviously higher than that of group CK, and that with the increase of drought gradient treatment, SOD and POD activity increased. SOD, POD activity in SS were obviously different with LS, MS. SOD and POD activity fluctuated at the beginning of drought treatment and SOD activity decreased and POD activity increased. In the whole water treatment period, LS, MS and SS had higher content of proline and soluble protein than that of CK, and increased with the extension of stress time. The content of proline and soluble protein in SS had obviously differences with LS, MS and that those had no differences in LS and MS. The content of proline decreased and soluble protein increased after treatment 80 days. The soluble sugar had no differences in group of LS, MS, SS. The content of membrane permeability and MDA content was higher than that of CK. The results had obviously differences in group of SS than LS, MS, that there was obvious positive correlation between membrane permeability and MDA content and there was obvious positive correlation MDA content and SOD activity.
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备注/Memo
收稿日期:2009-10-23。
基金项目:浙江海洋学院校级科研项目（21105006208）
作者简介:崔大练（1970- ），女，辽宁丹东人，主要从事水土保持研究工作。E-mail：hycdl@163.com
通讯作者:马玉心 （1965- ），男，教授，硕导，从事植物生理生态学研究。E-mail：donghai8883@163.com