GUO Wen,GAO Liwen,PENG Ziwei,et al.Characteristics of Microbial Community in Rhizosphere and Non-Rhizosphere Soil of Cunninghamia Lanceolata Plantation with Different Stand Ages[J].Research of Soil and Water Conservation,2022,29(06):260-267.
不同林龄杉木根际与非根际土壤微生物群落特征
- Title:
- Characteristics of Microbial Community in Rhizosphere and Non-Rhizosphere Soil of Cunninghamia Lanceolata Plantation with Different Stand Ages
- 文章编号:
- 1005-3409(2022)06-0260-08
- Keywords:
- Cunninghamia lanceolata; Rhizosphere and non-rhizosphere soil; phospholipid fatty acid(PLFA); soil microbial community
- 分类号:
- S714.3
- 文献标志码:
- A
- 摘要:
- 为探究中亚热带杉木土壤微生物群落随林龄变化特征,以中亚热带7,24,34 a生杉木人工林为研究对象,采用磷脂脂肪酸(PLFA)法分析其根际和非根际土壤微生物数量和群落结构及驱动土壤微生物变化的主要土壤环境因子。结果表明:随着杉木林龄的增长,非根际土壤各类微生物数量不断减少,根际土壤微生物数量不断增加,34 a生杉木人工林细菌含量、革兰氏阴性菌含量、Cy:MONO根际土壤显著高于非根际土壤,而其他各类微生物在根际和非根际土壤间均没有显著差异。相关分析和冗余分析结果表明:土壤环境因子对杉木土壤微生物群落有显著影响,其中有效磷和铵态氮含量对土壤微生物群落的影响较大,有效磷含量与土壤微生物群落呈正相关,土壤铵态氮含量与其呈负相关。因此,在杉木人工林管理过程中,可适当增加磷的输入,以增加土壤微生物数量,提高土壤质量,促进杉木的生长。
- Abstract:
- In order to explore the characteristics of the soil microbial communities of Cunninghamia lanceolata with forest stand age, in this study, Cunninghamia lanceolata plantation of various ages(7, 24, 34 years)were selected in the middle subtropics. Phospholipid fatty acid(PLFA)method was used to analyse the number and community structure of rhizosphere and non-rhizosphere soil microorganisms and the main soil environmental factors driving the changes of soil microbiome. The results showed that: With the increase of the age of the Cunninghamia lanceolate plantation, the number of microorganisms in non-rhizosphere soil decreased and increased. The contents of bacteria, Gram-negative bacteria and Cy:MONO in 34-year-old Cunninghamia lanceolata plantation were significantly higher than those in non-rhizosphere soil, but there was no significant difference in other microbiome between rhizosphere and non-rhizosphere soil. The results of redundancy analysis(RDA)showed that soil environmental factors had significant effects on the soil microbial community of Cunninghamia lanceolata, in which the contents of available phosphorus and ammonium nitrogen had great influence on soil microbial community, and the content of available phosphorus was positively correlated with soil microbial community. The content of soil ammonium nitrogen was negatively correlated with it. Therefore, in the process of Chinese fir plantation management, the input of phosphorus can be appropriately increased to increase the number of soil microorganisms, improve soil quality and promote the growth of Cunninghamia lanceolate.
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备注/Memo
收稿日期:2021-09-04 修回日期:2021-09-22
资助项目:国家自然科学基金(U1805243, 31700378)
第一作者:郭文(1995—),男,江西宜春人,硕士研究生,研究方向为森林理水与保土功能。E-mail:wenll20@163.com
通信作者:刘先(1985—),女,天津人,博士,讲师,主要从事土壤微生物生态学研究。E-mail:xianliu@fafu.edu.cn