CHEN Hanzhang,LIU Zhizhong.Effects of Simulated Nitrogen Deposition on Litter Decomposition and Soil Microbial Activities in Pinus massoniana Plantations[J].Research of Soil and Water Conservation,2020,27(01):73-80.
氮添加对马尾松人工林凋落物分解及其微生物活性的影响
- Title:
- Effects of Simulated Nitrogen Deposition on Litter Decomposition and Soil Microbial Activities in Pinus massoniana Plantations
- 文章编号:
- 1005-3409(2020)01-0073-08
- Keywords:
- Pinus massoniana plantations; litter decomposition; microbial activities; simulated nitrogen deposition
- 分类号:
- S714
- 文献标志码:
- A
- 摘要:
- 对马尾松人工林凋落物进行了12个月的模拟氮沉降试验[对照CK,0 kg N/(hm2·a)]、低氮[(LN,50 kg N/(hm2·a)]、中氮[MN,100 kg N/(hm2·a)]和高氮[HN,150 kg N/(hm2·a)],研究了凋落物分解特征、土壤微生物活性及其对模拟氮沉降的响应。结果表明:(1)随着氮浓度的增加,马尾松凋落物的分解系数呈先增加后降低趋势,MN处理下马尾松凋落物的分解系数达到最高,之后有所下降; 与对照相比,LN,MN,HN凋落物分解系数分别增加了15.36%,56.89%和12.97%;(2)模拟氮沉降促进了微生物量碳、氮、磷,相同月份微生物量碳、氮、磷均随着氮浓度的增加呈先增加后降低趋势,在MN处理下微生物量碳、氮、磷达到最大,HN处理下微生物量碳、氮、磷有所降低;(3)模拟氮沉降增加了大部分土壤酶的活性,HN处理下有所抑制,土壤中与碳(纤维素酶、纤维二糖水解酶、β-葡萄糖苷酶和β-木糖苷酶)、氮(硝酸还原酶)、磷(酸性磷酸酶和碱性磷酸酶)元素循环相关的酶均随着时间的增加呈增加趋势,相同月份在MN处理下达到最大,HN处理下有所降低。(4)不同氮处理下土壤微生物活度、微生物代谢熵、自养呼吸、异养呼吸、总呼吸变化趋势基本保持一致,随着时间的增加呈增加趋势,相同月份在MN处理下达到最大,HN处理下有所降低;(5)相关性分析表明纤维素酶、β-葡萄糖苷酶、β-木糖苷酶、硝酸还原酶与凋落物分解系数呈显著正相关(p<0.05),土壤微生物量碳、氮、微生物活度、微生物代谢熵和微生物总呼吸与凋落物分解系数呈显著正相关; PCA排序表明:土壤微生物呼吸和土壤微生物代谢熵很大程度上反映了其微生物特性,其中土壤碳循环酶、微生物活度、土壤微生物代谢熵对凋落物分解贡献最大。
- Abstract:
- This study was conducted in Pinus massoniana plantations to measure soil microbial activities and the effects of nitrogen deposition on litter decomposition. Nitrogen addition experiments were carried out within the forest in 2017. Four N addition treatments such as control, No N [CK, 0 kg N/(hm2·a)], low N [LN, 50 kg N/(hm2·a)], medium N [MN, 100 kg N/(hm2·a)] and High N [HN, 150 kg N/(hm2·a)] with three replicates were established in Pinus massoniana plantations. The results showed that:(1)simulated nitrogen deposition had a strong effect on litter decomposition and soil microbial activities in Pinus massoniana plantations; the litter decomposition coefficient increased at first and then decreased, and the largest litter decomposition coefficient was found in treatment of MN; the litter decomposition coefficients of treatments of LN, MN and HN increased by 15.36%, 56.89% and 12.97%, respectively, compared with CK;(2)simulated nitrogen deposition promoted soil microbial biomass carbon, nitrogen, phosphorus, which increased at first and then decreased, and the highest soil microbial biomass was observed in the treatment of MN;(3)simulated nitrogen deposition increased soil enzyme activity, soil enzyme activity was inhibited in the treatment of HN; the soil enzyme activity related to carbon, nitrogen, phosphorus increased at first and then decreased, and the highest soil enzyme activity was found in the treatment of MN;(4)soil microbial activity, microbial metabolic entropy, autotrophic respiration, heterotrophic respiration, total respiration showed the same change trend, which increased at first and then decreased, and the greatest values of these indicators were found in the treatment of MN;(5)correlation analysis indicated that there was a significant positive correlation between the decomposition coefficient of litter and the soil microbial biomass carbon, nitrogen, microbial activity, microbial metabolic entropy and total microbial respiration; soil microbial respiration and soil microbial metabolic entropy significantly reflected their microbial characteristics, among which soil carbon cycle enzyme, microbial activity and soil microbial metabolic entropy had the important contribution to the decomposition of litter.
参考文献/References:
[1] Reay D S, Dentener F, Smith P, et al. Global nitrogen deposition and carbon sinks[J]. Nature Geoscience, 2016,1(7):430-437.
[2] Stevens C J, Lind E M, Hautier Y, et al. Anthropogenic nitrogen deposition predicts local grassland primary production worldwide[J]. Ecology, 2016,96(6):1459-1465.
[3] Meunier C L, Gundale M J, Sánchez I S, et al. Impact of nitrogen deposition on forest and lake food webs in nitrogen-limited environments[J]. Global Change Biology, 2016,22(1):164-179.
[4] Simkin S M, Allen E B, Bowman W D, et al. Conditional vulnerability of plant diversity to atmospheric nitrogen deposition across the United States[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016,113(15):4086-4091.
[5] Hui L, Xu Z, Shan Y, et al. Responses of soil bacterial communities to nitrogen deposition and precipitation increment are closely linked with aboveground community variation[J]. Microbial Ecology, 2016,71(4):974-989.
[6] Song X, Li Q, Gu H. Effect of nitrogen deposition and management practices on fine root decomposition in Moso bamboo plantations[J]. Plant & Soil, 2017,410(1):207-215.
[7] Huang Z, Liu B, Davis M, et al. Long-term nitrogen deposition linked to reduced water use efficiency in forests with low phosphorus availability[J]. New Phytologist, 2016,210(2):431-442.
[8] Zhao Z, Dong S, Jiang X, et al. Effects of warming and nitrogen deposition on CH4, CO2, and N2 O emissions in alpine grassland ecosystems of the Qinghai-Tibetan Plateau[J]. Science of the Total Environment, 2017,592:565-572.
[9] Guerrieri R, Vanguelova E I, Michalski G, et al. Isotopic evidence for the occurrence of biological nitrification and nitrogen deposition processing in forest canopies[J]. Global Change Biology, 2016,21(12):4613-4626.
[10] Ren H, Chen Y C, Wang X T, et al.21 st-century rise in anthropogenic nitrogen deposition on a remote coral reef[J]. Science, 2017,356(6339):749-752.
[11] Gurmesa G A, Lu X, Gundersen P, et al. High retention of 15 N-labeled nitrogen deposition in a nitrogen saturated old-growth tropical forest[J]. Global Change Biology, 2016,22(11):3608-3620.
[12] Valliere J M, Allen E B. Interactive effects of nitrogen deposition and drought-stress on plant-soil feedbacks of Artemisia californica, seedlings[J]. Plant & Soil, 2016,403(1):277-290.
[13] Midgley M G, Phillips R P. Resource stoichiometry and the biogeochemical consequences of nitrogen deposition in a mixed deciduous forest[J]. Ecology, 2016,97(12):3369-3378.
[14] Pivovaroff A L, Santiago L S, Vourlitis G L, et al. Plant hydraulic responses to long-term dry season nitrogen deposition alter drought tolerance in a Mediterranean-type ecosystem[J]. Oecologia, 2016,181(3):1-11.
[15] Wang A Y, Wang M, Yang D, et al. Responses of hydraulics at the whole-plant level to simulated nitrogen deposition of different levels in Fraxinus mandshurica[J]. Tree Physiology, 2016,36(8):1045-1055.
[16] Lee J A, Caporn S J M. Ecological effects of atmospheric reactive nitrogen deposition on semi-natural terrestrial ecosystems[J]. New Phytologist, 1998,139(1):127-134.
[17] Matson P, Lohse K A, Hall S J. The globalization of nitrogen deposition:consequences for terrestrial ecosystems[J]. Ambio:A Journal of the Human Environment, 2002,31(2):113-120.
[18] Berg M P, Verhoef H A, Bolger T, et al. Effects of air pollutant-temperature interactions on mineral-N dynamics and cation leaching in reciplicate forest soil transplantation experiments[J]. Biogeochemistry, 1997,39(3):295-326.
[19] Ghee C, Neilson R, Hallett P D, et al. Priming of soil organic matter mineralisation is intrinsically insensitive to temperature[J]. Soil Biology & Biochemistry, 2013,66(11):20-28.
[20] 郭剑芬,杨玉盛,陈光水,等.森林凋落物分解研究进展[J].林业科学,2006,42(4):96-103.
[21] 曾锋,邱治军,许秀玉.森林凋落物分解研究进展[J].生态环境学报,2010,19(1):239-243.
[22] 李志安,邹碧,丁永祯,等.森林凋落物分解重要影响因子及其研究进展[J].生态学杂志,2004,23(6):77-83.
[23] 杨玉盛,陈光水,郭剑芬,等.杉木观光木混交林凋落物分解及养分释放的研究[J].植物生态学报,2002,26(3):275-282.
[24] 廖利平,高洪.外加氮源对杉木叶凋落物分解及土壤养分淋失的影响[J].植物生态学报,2000,24(1):34-39.
[25] 莫江明,薛璟花,方运霆.鼎湖山主要森林植物凋落物分解及其对N沉降的响应[J].生态学报,2004,24(7):1413-1420.
[26] 王其兵,李凌浩,白永飞,等.模拟气候变化对3种草原植物群落混合凋落物分解的影响[J].植物生态学报,2000,24(6):674-679.
[27] 李海涛,于贵瑞,李家永,等.井冈山森林凋落物分解动态及磷、钾释放速率[J].应用生态学报,2007,18(2):233-240.
[28] 宋新章,江洪,张慧玲,等.全球环境变化对森林凋落物分解的影响[J].生态学报,2008,28(9):4414-4423.
相似文献/References:
[1]黄从德,张国庆,唐宵,等.四川省马尾松人工林土壤有机碳密度研究[J].水土保持研究,2009,16(02):46.
HUANG Cong-de,ZHANG Guo-qing,TANG Xiao,et al.Study on Soil Organic Carbon Densities of Artificial Pinus massoniana in Sichuan Province[J].Research of Soil and Water Conservation,2009,16(01):46.
备注/Memo
收稿日期:2019-05-21 修回日期:2019-06-07
资助项目:福建省科技厅重点科技基金资助项目“重大危险性害虫桉树枝瘿姬小蜂控制关键技术及应用”(2012N0008)
第一作者:陈汉章(1962—),男,福建龙岩人,本科,副教授,研究方向:主要从事森林培育、城市湿地及林木病虫害研究。E-mail:Hanzhong_chen@126.com