XING Rongrong,WU Yonggui,LUO Youfa,et al.Effect of Pioneer Phytoremediation on Indigenous Zinc Smelting Slag Matrix Nutrients and Microbial Properties[J].Research of Soil and Water Conservation,2018,25(05):103-111.
先锋修复植物对土法炼锌废渣基质养分及微生物学特性的影响
- Title:
- Effect of Pioneer Phytoremediation on Indigenous Zinc Smelting Slag Matrix Nutrients and Microbial Properties
- 分类号:
- S154.3;X171.5
- 摘要:
- 为综合评价先锋修复植物对黔西北土法炼锌废渣基质的生态修复效应，通过野外采样及室内分析研究改良的土法炼锌废渣上自然定居植物土荆芥（Chenopodium ambrosioides）和大叶醉鱼草（Buddlejia davidii）以及先锋修复植物白三叶草（Trifolium repens）和黑麦草（Perennial ryegrass）对废渣基质的养分、酶活性及微生物活性的改善效果。结果表明：与无植被修复的裸露废渣相比，4种修复植物具有明显的根际效应，表现为根际废渣pH值降低，且4种修复植物可显著（p<0.05）增加根际废渣基质的养分（N，P，K、有机质）、酶活性（脲酶、蔗糖酶、磷酸酶、过氧化氢酶）、微生物活性（细菌、真菌、放线菌、微生物量、呼吸作用强度）。相关性分析表明，植物根际废渣养分、酶活性和微生物活性之间总体上呈正相关关系，其中有效氮与蔗糖酶和磷酸酶之间，全氮与细菌之间均呈极显著（p<0.01）相关，而有效钾与呼吸作用强度、微生物量和真菌之间，真菌与脲酶和蔗糖酶之间及微生物量与脲酶、蔗糖酶、磷酸酶、过氧化氢酶之间均呈显著相关（p<0.05）。通过主成分分析计算的综合肥力指标表明，三叶草修复铅锌冶炼废渣基质的综合肥力最高，而无植被存在的裸露废渣肥力指标最低。可见，在4种先锋修复植物中，三叶草作为先锋植物对土法炼锌废渣基质的修复效果最好。因此，在土法炼锌废渣堆场生态修复过程中，可选择三叶草作为先锋植物显著改良炼锌废渣的植生特性，为在废渣堆场上快速引进其他植物提供了有利条件。
- Abstract:
- In order to comprehensively evaluate the effect of ecological restoration of pioneer restoration plant on indigenous zinc smelting slag matrix, in northwest Guizhou. Through field sampling and laboratory analysis, the improvement effect of modified natural colonized plants (Chenopodium ambrosioides, Buddlejia davidii) and pioneer restoration plants (Trifolium repens, Perennial ryegrass) on residue matrix nutrient, enzyme activity and microbial activity was examined. The results showed that four pioneer restoration plants presented an obvious rhizosphere effect compared with the exposed slag without vegetation restoration, performance of rhizosphere slag decreased pH, and significantly increased (p < 0.05) residue matrix nutrients (N, P, K),organic matter, enzyme activities (urease, invertase, phosphatase, catalase), microorganism (bacteria, fungi, actinomycetes, microbial biomass, respiration intensity) of rhizosphere. Correlation analysis showed that residue matrix nutrients, enzyme activities and microbial activity of the four plants rhizosphere were positively correlated in general, the residue matrix available N was significantly correlated with residue matrix invertase and phosphatase, total nitrogen was significantly correlated with bacteria (p < 0.01). Available K had significant correlations with actinomycetes, microbial biomass and fungi, fungi had significant correlations with urease and invertase, microbial biomass was significant correlated with urease, invertase, phosphatase and catalase (p < 0.05). The results of principal component analysis showed that, the fertility of zinc smelting slag matrix repaired by Trifolium repens was the highest, the control was lowest. Obviously, Trifolium repens as to the repair effect of pioneer plant wastes residue matrix was the best in the four pioneer restoration plants. Therefore, in the process of ecological restoration of indigenous zinc smelting slag yard, using Trifolium repens as the characteristics of planting pioneer plants could significantly improve the zinc slag, provides favorable conditions for the rapid introduction of other plants in the waste residue yard.
参考文献/References:
[1] 王海峰,张元福,张中可.贵州锌工业现状及发展对策[J].贵州科学,2000,18(3):229-234.
[2] 吴攀,刘丛强,杨元根,等.土法炼锌废渣堆中的重金属及其释放规律[J].中国环境科学,2002,22(2):109-113.
[3] 林文杰,肖唐付,周晚春,等.黔西土法炼锌区Pb, Zn, Cd地球化学迁移特征[J].环境科学,2009,30(7):2065-2070.
[4] 吴烈善,莫小荣,曾东梅,等.废弃铅锌冶炼厂重金属污染场地的健康风险评价[J].生态毒理学报,2014,9(3):603-608.
[5] Zhang X W, Yang L S, Li Y H, et al. Impacts of lead/zinc mining and smelting on the environment and human health in China[J]. Environmental Monitoring and Assessment, 2012,184(4):2261-2273.
[6] 彭禧柱,杨胜香,李凤梅,等.3种工业有机废弃物对铅锌尾矿生物化学性质及植物生长的影响[J].环境科学,2016,37(1):301-308.
[7] 麻占威,吴永贵,付天岭,等.不同植物凋落物对土法冶炼铅锌废渣的改良效果[J].贵州农业科学,2014,42(6):188-192.
[8] 付天岭,吴永贵,曾艳,等.水可溶性有机物在铅锌废渣生态修复中的应用[J].环境工程学报,2017,1(2):1178-1184.
[9] 林文杰,肖唐付.土法炼锌区基质改良对刺槐生长的影响[J].生态与农村环境学报,2013,29(6):731-737.
[10] 邢丹,刘鸿雁,于萍萍,等.黔西北铅锌矿区植物群落分布及其对重金属的迁移特征[J].生态学报,2012,32(3):796-804.
[11] 刘建军,陈海滨,田呈明,等.秦岭火地塘林区主要树种根际微生态系统土壤性状研究[J].土壤侵蚀与水土保持学报,1998,4(3):52-57.
[12] Lynch J P. Root architecture and plant productivity[J]. Plant Physiology, 1995,109(1):7-13.
[13] 薛立,邝立刚,陈红跃,等.不同林分土壤养分、微生物与酶活性的研究[J].土壤学报,2003,40(2):280-285.
[14] 王友保,张莉,张凤美,等.大型铜尾矿库区节节草(Hippochaete ramosissimum)根际土壤重金属形态分布与影响因素研究[J].环境科学学报,2006,26(1):76-84.
[15] 江婧.根际微域重金属形态变化及其迁移转化研究[D].南昌:南昌大学,2012.
[16] 杨期和,刘惠娜,李清华,等.粤东铅锌尾矿区3种优势植物根际土壤微生物的活性研究[J].中国农学通报,2012,28(30):56-64.
[17] 鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000:30-106.
[18] 关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:274-340.
[19] 于鑫,张晓键,王占生.饮用水生物处理中生物量的脂磷法测定[J].给水排水,2002,28(5):1-5.
[20] 鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,1999:239-240.
[21] 程丽娟,薛泉宏.微生物学试验技术[M].2版.北京:科学出版社,2012:53-56.
[22] 宇传华. SPSS与统计分析[M].北京:电子工业出版社,2007:491-507.
[23] 张宏,沈章军,阳贵德,等.鸡粪改良铜尾矿对3种豆科植物生长及基质微生物量和酶活性的影响[J].生态学报,2011,31(21):6522-6531.
[24] 张福锁,曹一平.根际动态过程与植物营养[J].土壤学报,1992,29(3):239-250.
[25] 陈龙池,廖利平,汪思龙,等.根系分泌物生态学研究[J].生态学杂志,2002,21(6):57-62.
[26] Xian X. Effect of chemical forms of cadmium, zinc, and lend in polluted soils on their uptake by cabbage plant[J]. Plant and Soil, 1989,113(2):257-264.
[27] 孟令军,耿增超,殷金岩,等.秦岭太白山区6种中草药根际与非根际土壤化学性质及酶活性[J].应用生态学报,2012,23(10):2685-2692.
[28] 张学利,杨树军,张百习,等.不同林龄樟子松根际与非根际土壤的对比[J].福建林学院学报,2005,25(1):1-4.
[29] Yuan L, Huang J G, Yu S Q. Responses of nitrogen and related enzyme activities to fertilization in rhizosphere of wheat[J]. Pedosphere, 1997,7(2):141-148.
[30] 杨玉盛,何宗明,邹双全,等.格氏栲天然林与人工林根际土壤微生物及其生化特性的研究[J].生态学报,1998,18(2):198-202.
[31] 王笛,马风云,姚秀粉,等.黄河三角洲退化湿地土壤养分、微生物与土壤酶特性及其关系分析[J].中国水土保持科学,2012,10(5):94-98.
[32] 许景伟,王卫东,李成.不同类型黑松混交林土壤微生物、酶及其与土壤养分关系的研究[J].北京林业大学学报,2000,22(1):51-55.
[33] Diamantidis G, Effosse A, Potier P, et al. Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum[J]. Soil Biology and Biochemistry, 2000,32(7):919-927.
[34] 银秋玲,黄景,康轩,等.广西大明山土壤微生物数量及酶活性研究[J].南方农业学报,2012,43(4):472-476.
[35] 张星杰,刘景辉,李立军,等.保护性耕作方式下土壤养分、微生物及酶活性研究[J].土壤通报,2009,40(3):542-546.
[36] Nie M, Zhang X D, Wang J Q, et al. Rhizosphere effects on soil bacterial abundance and diversity in the Yellow River Deltaic ecosystem as influenced by petroleum contamination and soil salinization[J]. Soil Biology and Biochemistry, 2009,41(12):2535-2542.
[37] 王友保,张莉,沈章军,等.铜尾矿库区土壤与植物中重金属形态分析[J].应用生态学报,2005,16(12):2418-2422.
备注/Memo
收稿日期:2017-09-27;改回日期:2017-11-27。
基金项目:国家自然科学基金-贵州省联合资助项目（U1612442-3）；国家自然科学基金（41663009）；贵州省教育厅自然科学研究项目[黔教合KY字［2016]011］；贵州省教育厅研究生教育创新计划项目[黔教研合GZZ字［2016]07］
作者简介:邢容容(1992-),女,贵州盘县人,硕士研究生,研究方向为污染控制与生态修复。E-mail:xingrongrong19@163.com
通讯作者:吴永贵(1972-),男,贵州纳雍人,教授,博士生导师,博士,主要从事污染生态效应,污染生态控制及环境生态修复方面的教学研究工作。E-mail:ygwu72@126.com