SUN Bin,CHEN Wei-feng.Effect of Gibberellin on the Seed Germination and Seedling Growth of Puccinellia distans Under Salt-stress[J].Research of Soil and Water Conservation,2014,21(06):30-34.
赤霉素对盐害下碱茅的萌发和幼苗生长的影响
- Title:
- Effect of Gibberellin on the Seed Germination and Seedling Growth of Puccinellia distans Under Salt-stress
- Keywords:
- pratacultural science; salt stress; Puccinellia distans; gibberellic acid; salt resistance
- 分类号:
- S143.8
- 摘要:
- 以碱茅为试材，在100 mmol/L的NaCl盐胁迫下，通过种子培养、室内盆栽试验，研究盐胁迫下不同浓度赤霉素（8，15，20，25，50 mg/L）对碱茅种子萌发、幼苗生长的影响。结果表明：（1）100 mmol/L的NaCl严重抑制碱茅的种子萌发和幼苗生长，碱茅种子的发芽率、发芽势和发芽指数、根茎长显著降低，根系活力、过氧化物酶活力受到严重抑制；（2）在外源赤霉素溶液为8～20 mg/L的范围内，随着浓度的升高，碱茅种子的发芽率、发芽势和发芽指数、根茎长逐渐升高，根系活力、过氧化物酶活力、耐盐指数明显提高；（3）高浓度（ > 20 mg/L）的外源赤霉素浸种处理，对种子的促进作用逐渐下降，当赤霉素浓度为50 mg/L时，碱茅的发芽率和根茎比低于对照，产生了抑制效应。结论：不同浓度赤霉素对提高碱茅种子耐盐性有不同程度的促进作用，以赤霉素浓度为20 mg/L时作用效果最佳。
- Abstract:
- The Puccinellia distans seeds were treated with different gibberellin concentrations (8,15,20,25,50 mg/L) under the NaCl salt stress (100 mmol/L) in analogue experiments so as to study the influence on their seed germination and seedling growth. The results showed that:(1) 100 mmol/L NaCl significantly inhibited the seed germination and seedling growth, germination percentage, germination energy, germination index, root length and grass height significantly reduced, root activity, peroxidase activity and index of salinity tolerance were significantly inhibited, too; (2) during the range of 8～20 mg/L all the index data were improved; (3) but the promoting effect had fallen obviously when the the concentration of gibberellin is more than 20 mg/L.When the concentration of gibberellin is 50 mg/L, the seed germination and the ratio of Puccinellia distans roots and stems were lower than control sample, depression effect of gibberellin was obvious. Therefore, different concentrations of gibberellin have different promoting effects. The best promoting effect of gibberellin was found at the concentration of 20 mg/L.
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备注/Memo
收稿日期:2014-01-17;改回日期:2014-02-24。
基金项目:国家环保公益性行业科研专项任务（200809047）；山东省中青年科学家科研奖励基金（2007BSB08007）
作者简介:孙彬(1988-),女,山东青岛人,研究生,研究方向为土地生态整治、草地生态与管理研究。E-mail:zishanxiaomu@163.com
通讯作者:陈为峰(1970-),男,山东日照人,教授,博士,博士生导师,研究方向为土地生态整治、草地生态与管理研究。E-mail:chwf@sdau.edu.cn