ZHANG Qiong,LI Zhou-qi,HOU Wan-wei,et al.RAPD Analysis of Genetic Stability of Populus hopeiensis Tissue Culture Seeding from Different Regeneration Methods[J].Research of Soil and Water Conservation,2008,15(03):260-262.
河北杨不同再生途径组培苗遗传稳定性的RAPD检测
- Title:
- RAPD Analysis of Genetic Stability of Populus hopeiensis Tissue Culture Seeding from Different Regeneration Methods
- Keywords:
- Populus hopeiensis; regeneration method; somaclonal variation; RAPD
- 分类号:
- S792.11.05
- 摘要:
- 运用RAPD标记对河北杨不同培养途径获得的再生植株遗传稳定性进行检测，结果表明，以试管苗幼嫩叶片为外植体，通过直接不定芽发生途径获得的再生植株（20株）与对照株间表现出相同的条带数，说明此途径具有较高的遗传稳定性；以试管苗幼嫩叶片为外植体诱导愈伤组织，通过器官发生途径获得的再生植株（20株）中有2株分别在引物S268和引物S104中各出现一条多态性带，位点变异率为2/288，植株变异率为2/20，说明此培养途径过程中发生体细胞无性系变异，遗传稳定性较前者低，因此河北杨不同培养途径再生植株间存在遗传稳定性差异。
- Abstract:
- In this study,RAPD markers were used to analyze genetic stability of regenerated plant from different ways of Populus hopeiensis.The results indicated that,using the young leaves of vitro plantlets as explants,the RAPD bands were same between the control and regenerated plant which was from the direct regenerating adventitious buds.The young leaves of vitro plantlets were as explants to induce the callus,and there was a polymorphic band respectively in the primer S268 and S104 on 2 regenerated plant which were from the way of organogenesis.The mutational rate of RAPD sites and plants were 2/288 and 2/20 respectively.It indicated that there was somaclonal variation during training by this way,and the genetic stability was lower than by the former way.
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备注/Memo
收稿日期:2007-12-13。
基金项目:西北农林科技大学校专项“杨树体细胞无性系变异影响因素与调控技术的研究”
作者简介:张琼（1982- ），女，陕西宝鸡人，硕士研究生，主要从事林木遗传育种研究。E-mail：extreme0082000@yahoo.com.cn
通讯作者:李周岐（1962- ），男，陕西凤翔人，教授，博士生导师，主要从事林木生物技术育种研究。E-mail：lzhouqi@yahoo.com.cn