Quan Min,Du Xinpeng,Bi Yinli.Effects of AMF Inoculation on Root and Aggregate Stability of Three Plant Species in Open Pit Dump[J].Research of Soil and Water Conservation,2024,31(03):187-194.[doi:10.13869/j.cnki.rswc.2024.03.034]
露天矿排土场接种AMF对3种植物根系发育与土壤团聚体稳定性的影响
- Title:
- Effects of AMF Inoculation on Root and Aggregate Stability of Three Plant Species in Open Pit Dump
- 文章编号:
- 1005-3409(2024)03-0187-08
- Keywords:
- new dumping site; aggregate stability; root morphological characteristics; soil enzyme activity
- 分类号:
- S156
- 文献标志码:
- A
- 摘要:
- [目的]探究丛枝菌根真菌(AMF)对植物根系和土壤团聚体稳定性的影响规律,为露天矿排土场生态修复提供理论基础。[方法]对复垦3年的紫穗槐、沙棘、柠条3种植物的对照和接菌样地的土壤理化性质、土壤酶活性、植物根系形态、土壤团聚体稳定性等指标进行采样和测定,并进行相关性分析。[结果]3种植物接菌处理能够显著提高植物根系的平均直径,接菌样地是对照的1.33～2.24倍; 在压实土壤条件下,菌丝可能一定程度上代替植物细根系为植物提供水分和养分,使紫穗槐和沙棘样地接菌降低了根系的总根长。接菌显著提高了土壤酶活性,增加了土壤有机质含量,碱性磷酸酶活性最高提高了335.62%,脲酶活性最高提高了44.73%,易提取球囊霉素含量最高提高了82.56%,土壤有机质含量最高提高了64.74%。接菌通过增加土壤有机质含量,提高土壤酶活性等途径,显著增强了土壤团聚体的稳定性,平均重量直径提高了24.14%～34.29%,团聚体破坏率降低了18.25%～24.35%。[结论]露天矿排土场接种AMF能够显著提高土壤生物活性,提高养分含量,促进植物根系发育,从而提高土壤团聚体的稳定性。
- Abstract:
- [Objective] The aims of this study are to explore the effect of arbuscular mycorrhizal fungi AMF on the stability of plant roots and soil aggregates, and to provide a theoretical basis for the ecological restoration of open pit dump. [Methods] Soil physicochemical properties, soil enzyme activities, plant root morphology, soil aggregate stability and other indexes of 3 species of Amorpha fruticosa, Hippophae rhamnoides, Caragana korshinskii were sampled and measured in the control and inoculated plots. Correlation analysis was conducted for each index. [Results] The average root diameter of the three plants could be significantly increased by inoculation, and the inoculation plot was 1.33～2.24 times that of the control. Under the condition of compacted soil, mycelia to some extent provided water and nutrients for plants instead of fine roots, which reduced the total root length of root system. Inoculation significantly increased soil enzyme activity and soil organic matter content, alkaline phosphatase activity increased by 335.62%, urease activity increased by 44.73%, extractable balloon amycin content increased by 82.56%, soil organic matter content increased by 64.74%. By increasing soil organic matter content and soil enzyme activity, inoculation significantly enhanced the stability of soil aggregates, increased the average weight diameter by 24.14%～34.29%, and reduced the failure rate of aggregates by 18.25%～24.35%. [Conclusion] Inoculation of AMF in open pit dump can significantly improve soil biological activity, increase nutrient content, promote plant root development, and thus improve the stability of soil aggregates.
参考文献/References:
[1] 毕银丽.丛枝菌根真菌在煤矿区沉陷地生态修复应用研究进展[J].菌物学报,2017,36(7):800-806.
Bi Y L. Research advance of application of arbuscular mycorrhizal fungi to ecological remediation in subsided land of coal mining areas[J]. Mycosystema, 2017,36(7):800-806.
[2] 胡晶晶,毕银丽,龚云丽,等.接种AM真菌对采煤沉陷区文冠果生长及土壤特性的影响[J].水土保持学报,2018,32(5):341-345,351.
Hu J J, Bi Y L, Gong Y L, et al. Effects of arbusuclar mycorrhizal fungi on growth of Xanthoceras sorbifolia and soil properties in coal mining subsidence area[J]. Journal of Soil and Water Conservation, 2018,32(5):341-345,351.
[3] Lv X J, Wu X, Zhao Y L, et al. Drivers of spatio-temporal ecological vulnerability in an arid, coal mining region in Western China[J]. Ecological Indicators, 2019,106:105475.
[4] Ramani R V. Surface mining Technology:Progress and Prospects[J]. Procedia Engineering, 2012,46:9-21.
[5] 毕银丽,彭苏萍,杜善周.西部干旱半干旱露天煤矿生态重构技术难点及发展方向[J].煤炭学报,2021,46(5):1355-1364.
Bi Y L, Peng S P, Du S Z. Technological difficulties and future directions of ecological reconstruction in open pit coal mine of the arid and semi-arid areas of Western China[J]. Journal of China Coal Society, 2021,46(5):1355-1364.
[6] Nunez-Mir G C, Iannone B V, Curtis K, et al. Evaluating the evolution of forest restoration research in a changing world: A‘big literature' review[J]. New Forests, 2015,46(5/6):669-682.
[7] Steidinger B S, Crowther T W, Liang J, et al. Climatic controls of decomposition drive the global biogeography of forest-tree symbioses[J]. Nature, 2019,569(7756):404-408.
[8] Lei C, Booker F L, Cong T, et al. Arbuscular Mycorrhizal Fungi increase organic carbon decomposition under elevated CO2[J]. Science, 2012,337(6098):1084-1087.
[9] Watts-Williams S J, Cavagnaro T R. Nutrient interactions and arbuscular mycorrhizas:a meta-analysis of a mycorrhiza-defective mutant and wild-type tomato genotype pair[J]. Plant & Soil, 2014,384(1/2):79-92.
[10] Rillig M C, Mummey D L. Mycorrhizas and soil structure[J]. New Phytologist, 2006,171(1):41-53.
[11] Rillig M C, Wright S F, Eviner V T. The role of arbuscular mycorrhizal fungi and glomalin in soil aggregation:comparing effects of five plant species[J]. Plant and Soil, 2002,238:325-333.
[12] 钟思远,张静,褚国伟,等.南亚热带森林丛枝菌根真菌与土壤结构的关系[J].生态科学,2018,37(5):16-24.
Zhong S Y, Zhang J, Chu G W, et al. Soil aggregate composition and Its relationship with arbuscular mycorrhizal fungi in different restoration stages on severely eroded lands[J]. Ecological Science, 2018,37(5):16-24.
[13] 吴强盛,袁芳英,费永俊,等.菌根真菌对白三叶根际团聚体稳定性、球囊霉素相关土壤蛋白和糖类物质的影响[J].草业学报,2014,23(4):269-275.
Wu Q S, Yuan F Y, Fei Y J, et al. Effects of arbuscular fungi on aggregate stability, GRSP, and carbohydrates of white clover[J]. Acta Prataculturae Sinica, 2014,23(4):269-275.
[14] 冯固,张玉凤,李晓林.丛枝菌根真菌的外生菌丝对土壤水稳性团聚体形成的影响[J].水土保持学报,2001,15(4):99-102.
Feng G, Zhang Y F, Li X L. Effect of external hyphae of arbuscular mycorrhizal plant on water-stable aggregates in sandy soil[J]. Journal of Soil and Water Conservation, 2001,15(4):99-102.
[15] Rillig M C, Maestre F T, Lamit L J. Microsite differences in fungal hyphal length, glomalin, and soil aggregate stability in semiarid Mediterranean steppes[J]. Soil Biology and Biochemistry, 2003,35(9):1257-1260.
[16] Spohn M, Giani L. Impacts of land use change on soil aggregation and aggregate stabilizing compounds as dependent on time[J]. Soil Biology and Biochemistry, 2011,43(5):1081-1088.
[17] 速欢,王文龙,康宏亮,等.露天矿排土场平台—边坡系统侵蚀形态及径流产沙特征[J].应用生态学报,2020,31(9):3194-3206.
Su H, Wang W L, Kang H L, et al. Erosion morphology and the characteristics of runoff and sediment yielding in platform-slope system of opencast coal mine[J]. Chinese Journal of Applled Ecology, 2020,31(9):3194-3206.
[18] 肖栋,毕银丽,于立安,等.神华宝日希勒露天矿排土场生态修复中物种引种的关键气候因素[J].华南师范大学学报:自然科学版,2021,53(3):73-82.
Xiao D, Bi Y L, Yu L A, et al. Key climate factors in species introduction for ecological reconstruction of the dump site of Shenhua Baorixile open-pit mining[J]. Journal of South China Normal University(Natural Science Edition),2021,53(3):73-82.
[19] O'brien S L, Jastrow J D. Physical and chemical protection in hierarchical soil aggregates regulates soil carbon and nitrogen recovery in restored perennial grasslands[J]. Soil Biology and Biochemistry, 2013,61:1-13.
[20] Palumbo A V, Mccarthy J F, Amonette J E, et al. Prospects for enhancing carbon sequestration and reclamation of degraded lands with fossil-fuel combustion by-products[J]. Advance in Environmental Research, 2004,8(3/4):425-438.
[21] 李毅杰,梁强,董文斌,等.土壤压实对宿根甘蔗出苗及根系形成的影响[J].西南农业学报,2017,30(9):2041-2047.
Li Y J, Liang Q, Dong W B, et al. Effect of mechanical compaction on seedling emergence and roots formation of ratoon sugarcane[J]. Southwest China Journal of Agricultural Sciences, 2017,30(9):2041-2047.
[22] 蒲子天,张林,张弛,等.丛枝菌根真菌与植物共生影响植物水分状态的研究进展[J].土壤,2022,54(5):882-889.
Pu Z T, Zhang L, Zhang C, et al. Research progress of arbuscular mycorrhizal fungi and plant symbiosis affecting plant water regime[J]. Soils, 2022,54(5):882-889.
[23] 储薇,郭信来,张晨,等.丛枝菌根真菌—植物—根际微生物互作研究进展与展望[J].中国生态农业学报,2022,30(11):1709-1721.
Chu W, Guo X L, Zhang C, et al. Research progress and future directions of arbuscular mycorrhizal fungi-plantrhizosphere microbial interaction[J]. Chinese Journal of Eco-Agriculture, 2022,30(11):1709-1721.
[24] Olsson P A, Baath E, Jakobsen I. Phosphorus effects on the mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by analysis of fatty acid signatures[J]. Applied and Environmental Microbiology, 1997,63(9):3531-3538.
[25] 荣俊冬,凡莉莉,陈礼光,等.不同施氮模式和施氮量对福建柏幼苗生物量分配和根系生长的影响[J].林业科学,2020,56(7):175-184.
Rong J D, Fan L L, Chen L G, et al. Impacts on biomass allocation and root growth of fokienia hodginsii seedlings of different patterns and quantities of nitrogen application[J]. Scientia Silvae Sinicae, 2020,56(7):175-184.
[26] 高文礼,陈晓楠,伊力努尔·艾力,等.干旱及复水条件下接种AMF和根瘤菌对疏叶骆驼刺根系生长的影响[J].西北植物学报,2022,42(7):1189-1197.
Gao W L, Chen X N, Yilinuer·Aili, et al. Effect of double inculation of AMF and rhizobia on root growth of Alhagi sparsifolia under drought and rehydration conditions[J]. Acta Botanica Boreali-Occidentalia Sinica, 2022,42(7):1189-1197.
[27] 刘圆圆,张丽,王硕,等.氮和土著AMF对黄瓜间作土壤酶活性及氮利用的影响[J].菌物学报,2019,38(11):1965-1975.
Liu Y Y, Zhang L, Wang S, et al. Effects of nitrogen and inoculation of indigenous arbuscular mycorrhizal fungi on soil enzyme activity and nitrogen utilization of cucumber under intercropping conditions[J]. Mycosystema, 2019,38(11):1965-1975.
[28] 肖敏,凌婉婷,高彦征,等.丛枝菌根对菲芘污染土壤中几种酶活性的影响[J].农业环境科学学报,2009,28(5):919-924.
Xiao M, Ling W T, Gao Y Z. Effects of arbuscular mycorrhizae on enzymes in contaminated soils by phenanthrene and pyrene[J]. Journal of Agro-Enviroment Science, 2009,28(5):919-924.
[29] 雷卉,王久照,谭嫣,等.减量施肥和接种AMF对盆栽柑桔生长及土壤酶活性的影响[J].中国南方果树,2019,48(2):11-14,17.
Lei H, Wang J Z, Tan Y, et al. Effects of reduced fertilization and AMF inoculation on growth of potted citrus and soil enzyme activities[J]. South China Fruits, 2019,48(2):11-14,17.
[30] 张琪,方海兰,史志华,等.侵蚀条件下土壤性质对团聚体稳定性影响的研究进展[J].林业科学,2007,43(1):77-82.
Zhang Q, Fang H L, Shi Z H, et al. Advances in influence factors of aggregate stability under Erosion[J]. Scientia Silvae Sinicase, 2007,43(1):77-82.
[31] 秦笠,王宁,陈佳敏,等.不同恢复方式铁杆蒿群落土壤有机质和团聚体特征[J].生态学杂志,2023,42(6):1348-1354.
Qin L, Wang N, Chen J M, et al. Characteristics of soil organic matter and aggregates in Artemisia sacrorum community with different restoration types[J]. Chinese Journal Ecology, 2023,42(6):1348-1354..
[32] 周颖,李朝桢,郑听,等.贵州野生白及土壤物理性质与酶活性及相关性特征[J].西南农业学报,2022,35(2):336-342.
Zhou Y, Li C Z, Zheng T, et al. Physical properties, enzyme activity and correlation of soil for wild Bletilla striata in Guizhou[J]. Southwest China Journal of Agricultural Sciences, 2022,35(2):336-342.
[33] 董心亮,王金涛,田柳,等.盐渍化土壤团聚体和微生物与有机质关系研究进展[J].中国生态农业学报,2023,31(3):364-372.
Dong X L, Wang J T, Tian L, et al. Review of relationships between soil aggregates, microorganisms and soil organic matter in salt-affected soil[J]. Chinese Journal of Eco-Agriculture, 2023,31(3):364-372.
[34] 夏梓泰,赵吉霞,李永梅,等.周年轮作休耕模式对土壤球囊霉素和团聚体稳定性的影响[J].农业环境科学学报,2022,41(1):99-106.
Xia Z T, Zhao J X, Li Y M, et al. Effect of annual rotation and fallow pattern on the soil glomalin and aggregate stability[J]. Journal of Agro-Environment Science, 2022,41(1):99-106.
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备注/Memo
收稿日期:2023-05-27 修回日期:2023-06-23
资助项目:国家重点研发计划项目(2022YFF1303303); 国家自然科学基金(51974326)
第一作者:全民(1980—),男,内蒙古通辽人,学士,工程师,主要从事矿区生态修复研究。E-mail:863501813@qq.com
通信作者:毕银丽(1971—),女,陕西米脂人,博士,长江学者特聘教授,博士生导师,主要从事矿区生态修复研究。E-mail:ylbi88@126.com