Zhang Yanwen,Zhou Yajie,Zhang Buran,et al.Soli Enzymatic Stoichiometry and Nutrient Limitation During Revegetation in Sand-Fixing Forest[J].Research of Soil and Water Conservation,2023,30(06):102-111.[doi:10.13869/j.cnki.rswc.2023.06.050]
固沙林恢复土壤酶化学计量特征与养分限制效应
- Title:
- Soli Enzymatic Stoichiometry and Nutrient Limitation During Revegetation in Sand-Fixing Forest
- 文章编号:
- 1005-3409(2023)06-0102-10
- Keywords:
- extracellular enzyme activity; ecological enzyme stoichiometric; nutrient limitation; sand-fixation forest; Mu Us sandy land
- 分类号:
- S714
- 文献标志码:
- A
- 摘要:
- [目的]探究土壤胞外酶及酶化学计量比对沙漠化土地植被恢复过程的响应特征及其养分限制效应,可深入揭示人工固沙林重建植被过程养分转化能力与机理。[方法]基于时空替代法,以榆林毛乌素沙区的半固定沙地为(0 a)对照、选择恢复25～56 a灌木与乔木固沙林时间序列样地,测定分析了0—10 cm和10—20 cm土层的胞外酶活性、酶生态化学计量的演变特征及其限制微生物养分需求效应。[结果]随着植被恢复年限增加,两种林地土壤β-葡萄糖苷酶(BG)、β-1,4-乙酰氨基葡萄糖苷酶(NAG)、亮氨酸氨肽酶(LAP)和碱性磷酸酶(AP)活性在不同土层均呈显著升高趋势。但酶活性的不均衡变化也显著改变了土壤酶化学计量特征,56 a植被恢复过程中,土壤酶C:N,C:P,N:P呈增加趋势。土壤胞外酶化学计量比也表明植被恢复期间微生物生长受N限制显著增加,P限制并不明显,仅在乔木林恢复25 a时10—20 cm土层中出现P限制。[结论]固沙林植被恢复显著提升了土壤酶活性,增强了土壤碳氮代谢的能力,且在恢复过程中土壤微生物活动主要受到N养分的限制。
- Abstract:
- [Objective] This study was conducted to explore the response of soil extracellular enzymes and enzyme stoichiometric ratios to the revegetation process of desertified land and their nutrient limitation effects, which can provide insight into the nutrient conversion capacity and mechanism of the revegetation process of artificial sand-fixation forest. [Methods] Based on time-space substitution, the semi-fixed sandy land was sampled as 0 years control site, and the shrub and arbor sand-fixing forests with chronosequences of 25～56 years revegetation in Mu Us Sandy Land of Yulin were chosen. The evolution characteristics of enzyme activity and its stoichiometry, and resource limitation on microbial nutrient requirement in both 0—10 cm and 10—20 cm soil layers were analyzed. [Results] The activity of β-glucosidase(BG), β-1,4-acetaminoglucosidase(NAG), leucine aminopeptidase(LAP)and alkaline phosphatase(AP)in both soil layers under two forest lands increased continuously with year increase of vegetation restoration. However, unbalanced changes among the enzyme activities resulted in significant increase in soli enzymatic stoichiometry. The ratios of C:N, C:P and N:P calculated based on enzyme activity showed the increasing trend during 56 years of revegetation in both forest lands. And, significant increase in soli enzymatic stoichiometry reflected that microbial growth was limited by nitrogen(N)rather than phosphorus(P). Resource limitation by P only was observed in 10—20 cm soil layer in arbor land with 25 years of revegetation. [Conclusion] Revegetation in sand-fixing forest improved the enzyme activities and enhanced transformation of carbon and nitrogen in soil, and soil microbial activity was mainly limited by N nutrient during the vegetation restoration process.
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备注/Memo
收稿日期:2022-10-28 修回日期:2022-11-29
资助项目:陕西省自然科学基金重点项目(2023-JC-ZD-10); 陕西省农业专项资金项目[NYKJ-2022-YL(XN)16]; 国家大学生创新创业训练计划项目(202210712158)
第一作者:张延文(1997—),女,陕西咸阳人,硕士研究生,主要研究方向:人工林恢复碳循环。E-mail:15991082484@163.com
通信作者:佟小刚(1979—),男(锡伯族),新疆伊犁人,副教授,博士,主要从事人工林恢复生态效应与机制研究。E-mail:xiaogangtong@126.com