LIU Gengyuan,XIAO Jie,GAO Mingxia,et al.Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N2O Emission in Eum-Orthic Anthrosols[J].Research of Soil and Water Conservation,2022,29(02):99-105.
长期施肥对土nirS型反硝化细菌的影响及其与N2O排放的关系
- Title:
- Effect of Long-Term Fertilization on nirS-Type Denitrifer and Its Relationship with N2O Emission in Eum-Orthic Anthrosols
- 文章编号:
- 1005-3409(2022)02-0099-07
- 关键词:
- 长期施肥; nirS型反硝化细菌; 氧化亚氮; 实时定量PCR; 高通量测序
- Keywords:
- long-term fertilization; nirS-type denitrifier; nitrous oxide; quantitative real-time PCR; high-throughput sequencing
- 分类号:
- X144; X172
- 文献标志码:
- A
- 摘要:
- 土壤反硝化作用是土壤N2O产生的重要过程,亚硝酸盐还原酶(NIR)催化的亚硝态氮(NO-2)还原为一氧化氮(NO)是反硝化作用的关键环节,研究长期施肥对反硝化微生物的影响及其与N2O排放的关系对于全面理解土壤反硝化过程具有重要意义。基于28年的旱作雨养长期施肥试验,通过常规监测、定量PCR和高通量测序等探讨了长期不同施肥(不施肥CK、偏施肥的单施氮肥N和氮钾配施NK、以及氮磷钾平衡施肥NPK)下土N2O排放和nirS反硝化细菌群落特征及两者之间的关系。结果表明:长期化肥施用(N,NK和NPK)均显著提高了N2O累积排放量,其中平衡施肥(NPK)最高。长期化肥施用对nirS基因丰度和nirS型反硝化细菌的α-多样性无显著影响,但长期平衡施用化肥提高了uncultured_bacterium_2303和Rhodanobacter_sp._D206a的相对丰度,降低了unclassified_k_norank_d_Bacteria和unclassified_p_Proteobacteria的相对丰度,从而改变了nirS型反硝化细菌的群落结构组成。雨养旱作条件下,土壤有机碳(SOC)、全氮(TN)、有效磷(AP)和pH等土壤性质是土nirS型反硝化细菌群落结构组成变化的主要影响因素。土nirS型反硝化细菌群落结构组成对土壤N2O排放具有显著影响,而nirS基因丰度和nirS型反硝化细菌多样性并没有显著影响。
- Abstract:
- Soil denitrification is an important process for the production of soil N2O, and that Nitrite reductase(NIR)catalyzes the reduction of nitrite nitrogen(NO-2)to nitric oxide(NO)is the key step of denitrification. The study on the impact of long-term fertilization on denitrifying microorganisms and its relationship with soil N2O emissions is of great significance for a comprehensive understanding of the soil denitrification process. Based on 28 years of long-term fertilization experiments in rain-fed dry farming system, the characteristics of soil nirS-type denitrifying bacterial communities and N2O emission and their relationship were investigated under different types of long-term fertilization(No fertilization of CK, only N fertilization and NK combined fertilization of partial fertilizations, and balanced fertilization of NPK)by conventional monitoring, quantitative PCR and high-throughput sequencing. The results showed that all types of long-term fertilization(N, NK and NPK)significantly increased the cumulative N2O emission(EN2O), in which balanced fertilization(NPK)caused the the highest cumulative N2O emission; long-term fertilization had no significant effect on nirS gene abundance and the α-diversity of nirS-type denitrifying bacteria, while long-term balanced fertilization increased the relative abundance of uncultured_bacterium_2303 and Rhodanobacter_sp._D206a, and reduced the relative abundance of unclassified_k_norank_d_Bacteria and unclassified_p_Proteobacteria, thereby changed the community structure of nirS-type denitrifying bacteria. Soil properties such as soil organic carbon, total nitrogen, available phosphorusand pH are the main factors influencing the nirS-type denitrifying bacterial community structure that has a significant effect on soil N2O emission, while the abundance of nirS gene abundance and the diversity of nirS-type denitrifying bacteria have no significant effects in Eum-Orthic Anthrosols under rain-fed dry farming system.
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备注/Memo
收稿日期:2021-02-04 修回日期:2021-03-30
资助项目:国家高技术研究发展计划(2013AA102904); 陕西省自然科学基础研究计划项目(2018JM4007); 高等学校学科创新引智计划资助(B12007)
第一作者:刘耕苑(1995—),男,山西临汾人,硕士研究生,研究方向为土壤化学和土壤生态。E-mail:lgyhao0@163.com
通信作者:孙本华(1972—),男,江苏淮安人,教授,主要从事土壤化学和土壤生态研究。E-mail:sunbenhua@nwafu.edu.cn