[1]王凤娇,严加坤,张岁岐,等.矮秆基因Rht8和Rht13对冬小麦水分利用效率的效应比较[J].水土保持研究,2016,23(05):343-349.
　WANG Fengjiao,YAN Jiakun,ZHANG Suiqi,et al.Comparison of the Effects of Dwarfing Genes Rht8 and Rht13 on Water Use Efficiency of Winter Wheat[J].Research of Soil and Water Conservation,2016,23(05):343-349.
点击复制

矮秆基因Rht8和Rht13对冬小麦水分利用效率的效应比较

《水土保持研究》[ISSN:1005-3409/CN:61-1272/P] 卷: 23 期数: 2016年05期 页码: 343-349 栏目: 出版日期: 2016-10-28
Title:
Comparison of the Effects of Dwarfing Genes Rht8 and Rht13 on Water Use Efficiency of Winter Wheat
作者:
王凤娇1,2, 严加坤3, 张岁岐1,3, 胡银岗4
1. 西北农林科技大学 黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西 杨凌 712100;
2. 西北农林科技大学 生命科学学院, 陕西 杨凌 712100;
3. 中国科学院 水利部 水土保持研究所, 陕西 杨凌 712100;
4. 西北农林科技大学 农学院, 陕西 杨凌 712100
Author(s):
WANG Fengjiao1,2, YAN Jiakun3, ZHANG Suiqi1,3, HU Yingang4
1. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A & F University, Yangling, Shaanxi 712100, China;
2. College of Life Sciences, Northwest A & F University, Yangling, Shaanxi 712100, China;
3. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi 712100, China;
4. College of Agronomy, Northwest A & F University, Yangling, Shaanxi 712100, China
关键词:
冬小麦; 矮秆基因; 水分利用效率; 产量; 地上生物量
Keywords:
winter wheat; dwarfing gene; water use efficiency; yield; aboveground biomass
分类号:
Q945.79
摘要:
以含有矮秆基因Rht8的冬小麦品种晋麦47与Rht13的供体Magnif M1杂交获得的含有不同矮秆基因的重组自交系ZX44（Rht8）、ZX34（Rht13）、ZX33（Rht8+Rht13）和ZX16（rht）为材料，在田间和盆栽试验条件下主要研究了矮秆基因Rht8和Rht13对不同水分状况下冬小麦水分利用效率（WUE）和产量的影响。结果表明：含有矮秆基因的三个品系ZX34，ZX44和ZX33与高秆对照系（ZX16）在田间和盆栽同一水分条件下的耗水量差异不显著。田间与盆栽的干旱条件下四种小麦品系的产量趋势均为ZX44 > ZX34 > ZX16 > ZX33，灌水条件下均为ZX34 > ZX44 > ZX33 > ZX16。田间干旱条件下四种小麦品系的产量WUE为ZX44 > ZX34 > ZX16 > ZX33，灌水条件下为ZX44 > ZX34 > ZX33 > ZX16。盆栽两种水分条件下的产量WUE趋势均为ZX34 > ZX44 > ZX16 > ZX33。ZX34品系与ZX44品系的产量WUE在田间相同水分条件下、盆栽干旱条件下差异不显著，而在盆栽灌水条件下差异显著；两者的收获指数在田间和盆栽试验的干旱和灌水条件下均较高。田间相同水分处理下四种小麦品系的生物量WUE差异不显著，而盆栽相同水分条件下为ZX34 > ZX44 > ZX33 > ZX16。综上，引入Rht8基因和Rht13基因均能明显提高产量和WUE。在配合不断选育的基础上，含有Rht13基因的冬小麦是半干旱地区小麦高WUE育种中的另一材料。
Abstract:
Water shortage is a serious problem threatening sustainable agricultural development in Northwest China, where winter wheat (Triticum aestivum L.) is the largest water-consuming crop. The objective of this study was to explore the effects of dwarfing genes on the water use efficiency (WUE) and yield of winter wheat in Yangling area. Four recombinant inbred lines (ZX44: Rht8; ZX34: Rht13; ZX33: Rht8+Rht13 and ZX16: rht) of winter wheat Jinmai 47 (Rht8) and Magnif M1 (Rht13) were studied in the field and pot experiments under drought and irrigation conditions. The results showed that the water consumptions of ZX44, ZX34 and ZX33 lines had no significant difference from that of compared with control line (ZX16) under the same water condition in the field and pot experiments. The yields of four wheat lines in the field experiment under drought condition decreased in the order: ZX44 > ZX34 > ZX16 > ZX33, and their yields decreased in the order: ZX34 > ZX44 > ZX33 > ZX16 in the field experiment under irrigation condition. The trend of yields of four wheat lines in the field experiment under drought and irrigation conditions was consistent with them in the pot experiment under drought and irrigation condition. The yield WUE of four wheat lines at yield level in the field experiment under drought condition decreased in the order: ZX44 > ZX34 > ZX16 > ZX33, and decreased in the order of ZX44 > ZX34 > ZX33 > ZX16 under irrigation condition. The trend of yield WUE of four wheat lines in the pot experiment under both drought and irrigation condition decreased in the order: ZX34 > ZX44 > ZX16 > ZX33. The yield WUE of ZX34 line and ZX44 line had no significant difference under the same water condition in the field experiment and also had no significant difference under drought condition in the pot experiment, but the difference was significant under irrigation condition in the pot experiment. The harvest indices of ZX34 line and ZX44 line were higher under two water condition in the field and pot experiments. The WUE at aboveground biomass level of four wheat lines had no significant difference under the same water condition in the field experiment. The WUE at aboveground biomass level of four wheat lines in the pot experiment under both drought and irrigation conditions decreased the order: ZX34 > ZX44 > ZX33 > ZX16. ZX44 line and ZX34 line showed the highest yield WUE under the two water status in the field and pot experiments, respectively. Compared with the control, ZX44 and ZX34 lines presented higher WUE at aboveground biomass level under irrigation condition in the field treatment and appeared under two water status in the pot condition. In conclusion, the yield and WUE of winter wheat can be improved obviously by the introduction of Rht8 gene and Rht13 gene. Winter wheat carrying with Rht13 gene is another material of wheat breeding with high WUE in semi-arid areas on the basis of combining with continuous selection.

参考文献/References:

[1] 闫超,刘孟雨,董宝娣,等.小麦水分利用效率及相关性状QTL研究进展[J].中国农学通报,2009,25(8):92-95.
[2] 张正斌,徐萍,周晓果,等.作物水分利用效率的遗传改良研究进展学[J].中国农业科,2006,39(2):289-294.
[3] 于东海,李静,王秀芹,等.小麦矮秆新基因的RAPD标记[J].山东农业大学学报:自然科学版,2006,37(3):359-362.
[4] 贾继增,丁寿康,李月华,等.中国小麦的主要矮秆基因及矮源的研究[J].中国农业科学,1992,25(1):1-5.
[5] Worland A J, Korzun V, Roder M S, et al. Genetic analysis of the dwarfing gene Rht8in wheat[J]. PartⅡ. The distribution and adaptive significance of allelic variants at the Rht8locus of wheat as revealed by microsatellite screening[J]. Theoretical and Applied Genetics,1998,96(8):1110-1120.
[6] Mathews K L, Chapman S C, Trethowan R, et al. Global adaptation of spring bread and durum wheat lines near-isogenic for major reduced height genes[J]. Crop Science,2006,46(2):603-613.
[7] Sun Y, Wang X, Wang N, et al. Changes in the yield and associated photosynthetic traits of dry-land winter wheat (Triticum aestivum L.) from the 1940s to the 2010s in Shaanxi Province of China[J]. Field Crops Research,2014,167:1-10.
[8] Gale M D, Youssefian S. Dwarfing genes for wheat//Russell G E. Progress in Plant Breeding[M]. London:Butterworths and Co.,1985.
[9] 唐娜,姜莹,何蓓如,等.赤霉素敏感性不同矮秆基因对小麦胚芽鞘长度和株高的效应[J].中国农业科学,2009,42(11):3774-3784.
[10] Gasperini D, Greenland A, Hedden P, et al. Genetic and physiological analysis of Rht8 in bread wheat:an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids[J]. Journal of experimental botany,2012,63(12):4419-4436.
[11] Ellis M H, Rebetzke G J, Azanza F, et al. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat[J]. Theoretical and Applied Genetics,2005,111(3):423-430.
[12] Rebetzke G J, Ellis M H, Bonnett D G, et al. The Rht13dwarfing gene reduces peduncle length and plant height to increase grain number and yield of wheat[J]. Field Crops Research,2011,124(3):323-331.
[13] Rebetzke G J, Ellis M H, Bonnett D G, et al. Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.)[J]. Field Crops Research,2012,126:87-96.
[14] Richards R A. The effect of dwarfing genes in spring wheat in dry environments. I:Agronomic characteristics[J]. Crop and Pasture Science, 1992,43(3):517-527.
[15] Ehdaie B, Waines J G. Growth and transpiration efficiency of near-isogenic lines for height in a spring wheat[J]. Crop Science,1994,34(6):1443-1451.
[16] Wang Y, Chen L, Du Y, et al. Genetic effect of dwarfing gene Rht13 compared with Rht-D1b on plant height and some agronomic traits in common wheat(Triticum aestivum L.)[J]. Field Crops Research,2014,162:39-47.
[17] 李杏普,兰素缺,张京慧,等. Rht8,Rht10和Rht12矮秆基因对产量构成因子的影响[J].华北农学报,2009,24(Z2):72-75.
[18] Rebetzke G J, Richards R A, Fischer V M, et al. Breeding long coleoptile, reduced height wheats[J]. Euphytica,1999,106(2):159-168.
[19] Rebetzke G J, Richards R A. Giberellic acid-sensitive dwarfing genes reduce plant height to increase kernel number and grain yield of wheat[J]. Australian Journal of Agricultural Research,2000,51(2):235-246.
[20] 张学品,冯伟森,吴少辉,等.干旱胁迫对不同冬小麦品种水分利用效率及产量性状的影响[J].河南农业科学,2012,41(8):21-25,33.

相似文献/References:

[1]张燕,王百群,何瑞清.不同施肥下冬小麦生长过程中土壤矿质氮变化及其与冬小麦叶片SPAD值的关系[J].水土保持研究,2016,23(06):78.
　ZHANG Yan,WANG Baiqun,HE Ruiqing.Changes of Soil Mineral Nitrogen and Its Relationship with Soil and Plant Analyzer Development (SPAD) Values of Winter Wheat Leaves During Winter Wheat Growth Under Different Fertilization[J].Research of Soil and Water Conservation,2016,23(05):78.
[2]戚龙海,党廷辉,陈璐.旱塬冬小麦水分利用特征及对施肥的响应[J].水土保持研究,2009,16(03):105.
　QI Long-hai,DANG Ting-hui,CHEN Lu.The Water Use Characteristics of Winter Wheat and Response to Fertilization on Dry-land of Loess Plateau[J].Research of Soil and Water Conservation,2009,16(05):105.
[3]张燕青,刘文兆.不同施肥处理下麦田水分利用过程及其效率特征[J].水土保持研究,2012,19(05):133.
　ZHANG Yan-qing,LIU Wen-zhao.Characteristics of Water Utilization Process and Efficiency under Different Fertilization Treatments in a Wheat Field[J].Research of Soil and Water Conservation,2012,19(05):133.
[4]赵金忠,赵年武,赵恒和,等.贵德县近50年气候变化及对冬小麦生产潜力的影响[J].水土保持研究,2012,19(04):260.
　ZHAO Jin-zhong,ZHAO Nian-wu,ZHAO Heng-he,et al.Climatic Changes in the Past 50 Years in Guide County, Qinghai Province, and Their Influences on Potential Productivity of Winter Wheat[J].Research of Soil and Water Conservation,2012,19(05):260.
[5]刘耀宏,党增春,李鼎新.上黄试区冬小麦引种试验研究[J].水土保持研究,1996,3(01):81.
　Liu Yaohong,Dang Zengchun,Li Dingxin.Study on Seeds Selection of Winter Wheat in Shanghuang Experimental Field[J].Research of Soil and Water Conservation,1996,3(05):81.
[6]徐学选.冬小麦在固原试区的生态适应性[J].水土保持研究,1996,3(01):99.
　Xu Xuexuan.Ecological Adaptability of Winter Wheat in Guyuan Experimental Station[J].Research of Soil and Water Conservation,1996,3(05):99.
[7]张保军,杨文平,武科.天达-2116对渠灌区冬小麦生育及籽粒灌浆的影响[J].水土保持研究,2002,9(02):59.
　ZHANG Bao-jun,YANG Wen-ping,WU ke.Effect of Tianda-2116 on Growth and Grain Filling of Winter Wheat[J].Research of Soil and Water Conservation,2002,9(05):59.
[8]孙志强,王宗胜,鲍国军,等.施肥对黄土高原旱地冬小麦根系生长的影响[J].水土保持研究,2003,10(01):141.
　SUN Zhi-qiang,WANG Zong-sheng,BAO Guo-jun,et al.Effect of Fertilization on the Growth of Winter Wheat Roots in Dryland in Loses Plateau[J].Research of Soil and Water Conservation,2003,10(05):141.
[9]席聪梅,胡正祥,赵凤兰,等.豫西黄土丘陵区冬小麦化控抗倒伏试验研究[J].水土保持研究,2003,10(04):138.
　XI Cong-mei,HU Zheng-xiang,ZHAO Feng-lan,et al.Study on Lodging-resistance by Chemical Control of Winter Wheat in the Loess Hilly Area of West Henan Province[J].Research of Soil and Water Conservation,2003,10(05):138.
[10]邓洁,陈静,贺康宁.灌水量和灌水时期对冬小麦耗水特性和生理特性的影响[J].水土保持研究,2009,16(02):191.
　DENG Jie,CHEN Jing,HE Kang-ning.Effect of Irrigation Amount and Stage on Water Consumption Characteristics and Physiological Characteristics of Winter Wheat[J].Research of Soil and Water Conservation,2009,16(05):191.

备注/Memo

收稿日期:2016-01-11;改回日期:2016-02-18。
基金项目:国家科技支撑计划“黄土高原旱区增粮增效潜力与提升技术研究”（2015BAD22B01）
作者简介:王凤娇(1990-),女,新疆阿克苏人,硕士研究生,主要从事植物生理生态研究。E-mail:fengjiaow@126.com
通讯作者:张岁岐(1966-),男,陕西岐山人,研究员,博士生导师,主要从事植物水分生理生态研究。E-mail:sqzhang@ms.iswc.ac.cn

更新日期/Last Update: 1900-01-01