LI Yan-jie,LIU Ren-hua,YANG Jun-nian,et al.Dynamics of Enzyme Activities of Cynodon Dactylon Roots from Hydro-Fluctuation Belt in the Three Gorges Reservoir Area during Flooding[J].Research of Soil and Water Conservation,2014,21(03):288-292.
水淹胁迫下三峡库区野生狗牙根根系酶活性变化
- Title:
- Dynamics of Enzyme Activities of Cynodon Dactylon Roots from Hydro-Fluctuation Belt in the Three Gorges Reservoir Area during Flooding
- 分类号:
- Q948
- 摘要:
- 三峡库区消落带治理的重点方向是筛选消落带适生植物，故研究已筛选的适生植物狗牙根在水淹生境下抗胁迫相关酶活性变化对于消落带的生态治理具有重要意义。以三峡库区自然消落带野生狗牙根（XC）和非消落带野生狗牙根（FC）为研究对象，采用野外采样和对比分析等手段研究了三峡库区自然消落带野生狗牙根（XC）在不同水淹深度的抗胁迫酶活性变化。结果显示：①随着水淹深度增加，XC组狗牙根根系MDA含量均呈递增趋势，表明狗牙根在深淹胁迫下胁迫程度逐渐加剧。② XC组狗牙根根系乙醇脱氢酶（ADH）、谷胱甘肽还原酶（GR）、过氧化物酶（POD）、超氧化物歧化酶（SOD）的酶活性和游离脯氨酸含量较对照均有增加；ADH、POD和SOD酶活性最高值出现在水淹深度为8 m，而GR酶活性的最高值出现在水淹深度为4 m。③水淹期间，XC组狗牙根根系淀粉含量基本和对照同水平，可溶性糖含量较对照有所降低，表明水淹期间狗牙根处于低代谢和高储能状态。从水淹胁迫下抗胁迫酶活性变化角度解释了筛选到的野生狗牙根是三峡水库消落带植被恢复的适生物种。
- Abstract:
- Screening suitable plants is the key to management of Three Gorges Reservoir Area, so exploring dynamics of anti-flooded enzyme activities of suitable Cynodon dactylon to flooding has the important significance for ecological management of hydro-fluctuation belt. With the water-level-fluctuation zone (XC) and non-water-level-fluctuation zone (FC) of Three Gorges Reservoir Area were chosen as the key research sites. Enzyme activities of anti-flooded of XC group to different water depth was studied by field sampling and comparative analysis. The results showed: ① MDA content of Cynodon dactylon root in the XC group increased with the increase of water depth, which showed deep submergence stress was aggravated; ② activitives of alcohol dehydrogenase (ADH), glutathione reductase (GR), peroxidase (POD), superoxide dismutase (SOD) and content of proline in the XC group increased, compared to the control; the peak activity of ADH, POD and SOD appeared in the 8 m water depth, and the peak activity of GR was in the 4 m water depth; ③ during the flooding period, Cynodon dactylon was in low metabolism and high energy storage since the content of starch was the same as control level and the content of soluble sugar decreased. The results showed that Cynodon dactylon was probably regarded as suitable species of management of hydro-fluctuation in Three Gorges Reservoir Area with respect to the physiological responses to flooding stress.
参考文献/References:
[1] 杨乐,张烨,侯培强,等.三峡水库中下游水体氮磷时空变化与机制分析[J].长江流域资源与环境,2012,21(6):732-738.
[2] 苏维词,赵纯勇,杨华.三峡库区消落区自然条件及其开发利用评价[J].地理科学,2009,29(2):268-272.
[3] 钟冰,唐治诚.三峡库区水土流失及其防治[J].水土保持研究,2001,8(2):147-149.
[4] 王海锋,曾波,李娅,等.长期完全水淹对4种三峡库区岸生植物存活及恢复生长的影响[J].植物生态学报,2008,32(5):977-984.
[5] 王海锋,曾波,乔普,等.长期水淹条件下香根草(Vetiveria zizanioides),菖蒲(Acorus calamus)和空心莲子草(Alternanthera philoxeroides)的存活及生长响应[J].生态学报,2008,28(6):2571-2580.
[6] 谭淑端,张守君,张克荣,等.长期深淹对三峡库区三种草本植物的恢复生长及光合特性的影响[J].植物科学学报,2009,27(4):391-396.
[7] 刘云峰,刘正学.三峡水库消落区极限条件下狗牙根适生性试验[J].西南农业大学学报,2005,27(5):661-663.
[8] 马利民,唐燕萍,张明,等.三峡库区消落区几种两栖植物的适生性评价[J].生态学报,2009,29(4):1885-1892.
[9] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.
[10] Cardoso S M, Pereira C, Oliveira C R. Mitochondrial function is differentially affected upon oxidative stress[J]. Free Radical Biology and Medicine,1999,26(1):3-13.
[11] 罗祺,张纪林,郝日明,等.水淹胁迫下10个树种某些生理指标的变化及其耐水淹能力的比较[J].植物资源与环境学报,2007,16(1):69-73.
[12] 谭淑端,朱明勇,党海山,等.三峡库区狗牙根对深淹胁迫的生理响应[J].生态学报,2009,29(7):3685-3691.
[13] Zhang G, Tanakamaru K, Abe J, et al. Influence of waterlogging on some anti-oxidative enzymatic activities of two barley genotypes differing in anoxia tolerance[J]. Acta Physiologiae Plantarum,2007,29(2):171-176.
[14] Biemelt S, Keetman U, Albrecht G. Re-aeration following hypoxia or anoxia leads to activation of the antioxidative defense system in roots of wheat seedlings[J]. Plant Physiology,1998,116(2):651-658.
[15] Yan B, Dai Q, Liu X, et al. Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves[J]. Plant and Soil,1996,179(2):261-268.
[16] Crawford R, Braendle R. Oxygen deprivation stress in a changing environment[J]. Journal of Experimental Botany,1996,47(2):145-159.
[17] Komatsu S, Thibaut D, Hiraga S, et al. Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots[J]. Plant Molecular Biology,2011,77(3):309-322.
[18] Strommer J. The plant ADH gene family[J]. The Plant Journal,2011,66(1):128-142.
[19] Balakhnina T, Bennicelli R, Stêpniewska Z, et al. Stress responses of spring rape plants to soil flooding[J]. International Agrophysics,2012,26:347-353.
[20] 施美芬,曾波,申建红,等.植物水淹适应与碳水化合物的相关性[J].植物生态学报,2010,34(7):855-866.
相似文献/References:
[1]徐泉斌,傅瓦利,孙璐,等.三峡库区消落带土壤抗蚀性研究[J].水土保持研究,2009,16(05):13.
XU Quan-bin,FU Wa-li,SUN Lu,et al.Study on Soil Anti-erodibility for the Water Level-fluctuating Zone in Three Gorges Reservoir Area[J].Research of Soil and Water Conservation,2009,16(03):13.
[2]王勇,李鹏,穆军,等.金沙江干热河谷水电站库区消落带生态修复对策研究[J].水土保持研究,2009,16(05):141.
WANG Yong,LI Peng,MU Jun,et al.Ecological Restoration in Level-fluctuating Zone Countermeasures of Dry-hot Valleys Reservoirs in the Jinsha River[J].Research of Soil and Water Conservation,2009,16(03):141.
[3]王晓阳,傅瓦利,谢芳,等.三峡库区消落带完整淹水后土壤重金属分布特征及其影响因素[J].水土保持研究,2010,17(06):267.
WANG Xiao-yang,FU Wa-li,XIE Fang,et al.The Distribution Characteristic of Soil Heavy Metals and Their Influencing Factors in Water-level-fluctuating Zones of the Three-Gorges Reservoir Area[J].Research of Soil and Water Conservation,2010,17(03):267.
[4]钟荣华,鲍玉海,贺秀斌,等.三峡库区消落带几种草地根系分布及土壤养分变化[J].水土保持研究,2015,22(02):151.
ZHONG Ronghua,BAO Yuhai,HE Xiubin,et al.Root Distributions of Several Grasslands and Soil Nutrient Variation in the Riparian Zone of Three Gorges Reservoir[J].Research of Soil and Water Conservation,2015,22(03):151.
[5]贾国梅,席颖,许文年,等.三峡库区消落带土壤酶活性特征[J].水土保持研究,2015,22(04):24.
JIA Guomei,XI Ying,XU Wennian,et al.Characteristics of the Activities of Soil Enzymes in Riparian Zone of Three Gorges Reservoir Area[J].Research of Soil and Water Conservation,2015,22(03):24.
[6]李铭怡,刘刚,许文年,等.新银合欢在不同土壤水分条件下的适生性研究[J].水土保持研究,2013,20(02):259.
LI Ming-yi,LIU Gang,XU Wen-nian,et al.Research on Adaptability of Leucanea leucocephala under Different Soil Moisture Conditions[J].Research of Soil and Water Conservation,2013,20(03):259.
[7]鲍玉海,贺秀斌,钟荣华,等.三峡水库消落带植被重建途径及其固土护岸效应[J].水土保持研究,2014,21(06):171.
BAO Yu-hai,HE Xiu-bin,ZHONG Rong-hua,et al.Revegetation and Its Effects on Soil Reinforcement in the Riparian Zone of Three-Gorge Reservoir[J].Research of Soil and Water Conservation,2014,21(03):171.
[8]钟荣华,吕发友,鲍玉海,等.三峡水库消落带草本植物对若干土壤物理性质的影响[J].水土保持研究,2018,25(01):17.
ZHONG Ronghua,LYU Fayou,BAO Yuhai,et al.Impacts of Several Grasses on Soil Physical Properties in the Riparian Zone of Three Gorges Reservoir[J].Research of Soil and Water Conservation,2018,25(03):17.
[9]吕发友,唐强,张淑娟,等.三峡水库消落带紫色土物理性质对反复淹水作用的响应[J].水土保持研究,2018,25(01):276.
LYU Fayou,TANG Qiang,ZHANG Shujuan,et al.Response of Purple Soil Physical Properties to Repeated Water Flooding in Water-Level Fluctuation Zone in the Three Gorges Reservoir[J].Research of Soil and Water Conservation,2018,25(03):276.
[10]鲍玉海,郭丰,胡保生,等.西南干旱河谷区水库消落带柳树造林效果评价[J].水土保持研究,2018,25(05):216.
BAO Yuhai,GUO Feng,HU Baosheng,et al.Assessment on the Effect of the Afforestation With Willow Along the Reservoir Riparian Zone in the Dry Valley Area of Southwest China[J].Research of Soil and Water Conservation,2018,25(03):216.
备注/Memo
收稿日期:2013-10-10;改回日期:2013-10-23。
基金项目:重庆市科委自然科学基金计划资助项目（cstc2012jjA80009）；重庆三峡学院项目（2008-sxxyqn-31）
作者简介:李彦杰(1979-),男,陕西杨凌人,讲师,博士研究生,主要研究方向为三峡库区生态与环境保护。E-mail:jacoblee@yeah.net