WANG Bo,LI Kai-rong,CUI Bi-xiao,et al.Reponse of Anti-oxidation Protective System of Robinia pseudoacacia Seedlings to Petroleum Contamination[J].Research of Soil and Water Conservation,2014,21(02):251-256.
刺槐苗木抗氧化保护系统对石油污染的响应
- Title:
- Reponse of Anti-oxidation Protective System of Robinia pseudoacacia Seedlings to Petroleum Contamination
- Keywords:
- R.pseudoacacia seedings; anti-oxidation protective system; petroleum contamination; phytoremediation
- 分类号:
- S718.43;X503.23
- 摘要:
- 为探究刺槐（Robinia pseudoacacia）抗氧化保护系统在石油污染逆境下的适应性调节机制，采用盆栽试验研究了0，5，10，15，20 g/kg浓度石油处理下1年生刺槐苗木的抗氧化保护酶系、抗氧化剂以及过氧化氢H2O2和丙二醛MDA含量的动态变化。结果表明：在5~20 g/kg石油浓度处理30 d时，除抗坏血酸过氧化物酶（APX）外，刺槐苗木的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽还原酶（GR）活性和还原型谷胱甘肽（GSH）含量较对照显著降低，抗坏血酸（AsA）和类胡萝卜素（Car）含量则基本维持稳定，而在20 g/kg石油浓度下显著增加；随石油污染浓度的增加和时间延长（60~90 d），SOD、CAT活性和AsA、GSH含量基本高于或等于对照，APX、GR活性以及Car含量则随石油浓度增加显著降低。石油污染后刺槐苗木H2O2较对照显著增加，MDA含量均等于或显著低于对照水平。刺槐苗木抗氧化保护系统在20 g/kg浓度以内的石油处理下受损伤程度不明显。维持抗氧化剂含量以及抗氧化保护酶系—抗氧化剂的协同作用分别为刺槐石油污染短期和长期内清除活性氧的主要机制。
- Abstract:
- In order to investigate the self-adjusting adaptability mechanism of the anti-oxidation protective system of Robinia pseudoacacia seedings under petroleum contamination stress, the dynamic changes of anti-oxidation enzymes, antioxidants and the contents of hydrogenperoxide H2O2 and malondialdehyde MDA of one year old Robinia pseudoacacia seedlings in pot experiment were measured. The results showed that except ascrodate peroxidase (APX) activity, the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) and glutathione (GSH) content of Robinia pseudoacacia seedings were significantly lower than the control under the stress of 5~20 g/kg petroleum contamination for 30 days. The contents of ascorbicacid (AsA) and carotenoid (Car) remained stable at the same conditions and they became significantly higher than CK after stressed by 20 g/kg petroleum contamination for 30 days. Along with the increase of petroleum concentration and the stress duration (60~90 d), SOD and CAT activities and AsA and GSH contents generally became higher or equal to control level, while APX and GR activities and Car content decreased significantly over the increase of petroleum concentration. After stressed by petroleum contamination, H2O2 contents of seedings significantly increased while MDA contents were equal to or lower than that of control. The destruction of anti-oxidation protective system of Robinia pseudoacacia seedings of petroleum contamination below 20 g/kg concentration was not obvious. Antioxidants content maintenance and the synergistic effects of anti-oxidation enzymes and antioxidants could be obsverved as the main mechanisms under short- and long-term of petroleum contamination stress, respectively.
参考文献/References:
[1] 夏会龙,吴良欢,陶勤南.有机污染环境的植物修复研究进展[J].应用生态学报,2003,14(3):457-460.
[2] 时腾飞,刘增文,田楠,等.黄土区石油污染对土壤及豆科灌草植物的潜在影响[J].草地学报,2013,21(2):295-301.
[3] 陈嫣,李广贺,张旭,等.石油污染土壤植物根际微生态环境与降解效应[J].清华大学学报:自然科学版,2005,45(6):784-787.
[4] Li X, Feng Y, Sawatsky N. Importance of soil-water relations in assessing the endpoint of bioremediated soils[J]. Plant and Soil,1997,192(2):219-226.
[5] Spriggs T, Banks M K, Schwab P. Phytoremediation of polycyclic aromatic hydrocarbons in manufactured gas plant-impacted soil[J]. Journal of Environmental Quality,2005,34(5):1755-1762.
[6] 宋雪英,宋玉芳,孙铁珩,等.石油污染土壤植物修复后对陆生高等植物的生态毒性[J].环境科学,2006,27(9):1866-1871.
[7] 高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:210-226.
[8] 王生耀,王堃,赵永来,等.干旱和UV-B对两种牧草生长和抗氧化系统的影响[J].草地学报,2008,16(4):392-395,402.
[9] 赵丽英,邓西平,山仑.活性氧清除系统对干旱胁迫的响应机制[J].西北植物学报,2005,25(2):413-418.
[10] 杨文权,寇建村,雷忠萍.小冠花抗氧化保护系统对干旱胁迫及复水的动态响应[J].草地学报,2013,21(2):316-321.
[11] Zhang J, Kirkham M B. Antioxidant responses to drought in sunflower and sorghum seedlings[J]. New Phytologist,1996,132(3):361-373.
[12] 王娟,李德全.水分胁迫下植物体内的抗氧化剂及其作用[J].生物学通报,2002,37(10):22-23.
[13] 韩刚,党青,赵忠.干旱胁迫下沙生灌木花棒的抗氧化保护响应研究[J].西北植物学报,2008,28(5):1007-1013.
[14] 弓晓峰,荣亮,杨丽珍,等.石油污染土壤对玉米生长的影响及其生态毒性研究[J].环境科学与技术,2011,34(10):71-75.
[15] 李春荣,王文科,曹玉清,等.石油污染土壤对黄豆生长的生态毒性效应[J].西北农林科技大学学报:自然科学版,2008,36(1):116-120.
[16] 王雪峰,陈桂珠,许夏玲.白骨壤对石油污染的生理生态响应[J].生态学报,2005,25(5):1095-1100.
[17] 庄飞,范阳阳,魏瑞峰.土壤水分和氮素有效性对刺槐幼苗叶片活性氧产生和清除的影响[J].西北植物学报,2013,33(6):1190-1196.
[18] 岳冰冰,李鑫,任芳菲,等.石油污染对紫花苜蓿部分生理指标的影响[J].草业科学,2011,28(2):236-240.
备注/Memo
收稿日期:2013-10-19;改回日期:2013-11-06。
基金项目:林业公益性行业科研专项（201104002-4）；西北农林科技大学基本科研业务费专项“陕北主要造林树种对石油污染的耐性研究”（QN2011162）
作者简介:王波(1987-),男,陕西耀州区人,硕士研究生,主要从事水土保持与生态工程研究。E-mail:bowang2007@163.com
通讯作者:李凯荣(1955-),男,陕西扶风人,教授,主要从事水土保持与生态工程研究。E-mail:lkrzhxy@nwsuaf.edu.cn