AN Yu,SHEN Ying-bai,ZHANG Zhi-xiang.Effects of Damage Stress on Membrane Lipid Peroxidation and Antioxidant Enzymes in Poplar Leaves[J].Research of Soil and Water Conservation,2010,17(05):241-244.
伤害胁迫对合作杨叶片膜质过氧化及抗氧化酶活性的影响
- Title:
- Effects of Damage Stress on Membrane Lipid Peroxidation and Antioxidant Enzymes in Poplar Leaves
- 分类号:
- Q946
- 摘要:
- 本研究初步探讨了合作杨抗虫性与过氧化氢（H2O2）含量、丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性变化的关系。发现昆虫取食和机械损伤均可以引起叶片中H2O2和MDA含量的积累及相应的抗氧化酶SOD，CAT，POD活性提高。两种不同处理方法中，昆虫取食诱导的膜质过氧化和抗氧化酶活性变化（除了CAT活性）与机械损伤诱导结果表现出明显差异。这些结果表明，昆虫取食和机械损伤可以诱导叶片中H2O2和MDA积累，且其代谢受到抗氧化酶活性变化的共同影响，通过提高抗氧化酶活性可以减轻膜质过氧化程度，有效地诱导伤害防御反应的产生。同时，两种处理之间的差异表明H2O2，SOD，CAT和POD与合作杨抗虫性之间存在一定的关联。
- Abstract:
- The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were measured in poplar leaves damaged by mechanical damage and herbivore wounding. The results showed that the concentration of H2O2 and MDA and the activities of SOD, CAT and POD were enhanced; the change of membrane lipid peroxidation and antioxidant enzymes was significantly higher in the herbivore wounding treatment (except CAT). The results suggest that the concentration of H2O2 and MDA were closely related to the change in antioxidant enzymes activities. A high level of antioxidant enzymes could decrease membrane lipid peroxidation levels and effectively induce plant defense responses. Meanwhile, the difference between mechanical damage and herbivore wounding suggest that H2O2, SOD, CAT and POD are associated with insect resistance in poplar.
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备注/Memo
收稿日期:2010-6-19;改回日期:2006-3-16。
基金项目:国家“十一五”科技支撑计划课题（2006BAD01A15；2006BAD24B04）
作者简介:安钰（1982- ），女，宁夏固原人，博士，助理研究员，主要从事植物生理生态学研究。E-mail：anyu-02@163.com
通讯作者:沈应柏（1959- ），男，云南宣威人，博士，教授，博士生导师，主要从事植物生理生态学研究。E-mail：ybshen@bjfu.edu.cn