ZENG Xiao-hong,WU Jian-rong,MA Huan-cheng,et al.Study on Drought-hot Tolerance of Rhizobia Strains of Legume Isolated from Jinshajiang River Dry-hot Valley[J].Research of Soil and Water Conservation,2006,13(04):75-77.
金沙江河谷地区豆科树种根瘤菌耐干热研究
- Title:
- Study on Drought-hot Tolerance of Rhizobia Strains of Legume Isolated from Jinshajiang River Dry-hot Valley
- Keywords:
- rhizobia; tolerance to drought-hot; dry-hot simulation
- 分类号:
- Q948
- 摘要:
- 本实验在人工设置高温和模拟干旱条件下,对分离自金沙江河谷地区的12株豆科树种根瘤菌菌株进行耐干热分析.结果显示,12株供试菌株对干热表现出不同的耐受性,大部分菌株如:P5-1、P5-6、P5-8、P6-11、Y8-12、P5-5、Y6-13、P5-7能忍受40℃的高温;在人工模拟干旱的高渗环境中,也有部分菌株(P5-1、P5-2、P5-6、P5-7)表现出极强的耐受能力.通过本次的耐性研究,发现菌株P5-1、P5-6、P5-7不仅能在40℃的高温下生长而且在-8.71×105
- Abstract:
- In this study,12 strains isolated from the root nodules of woody legumes in the various regions of Jinshajiang River dry-hot valley were used to study the drought-hot tolerance by adding Polythylene glycol 6000 to YME brouth medium and controlling the temperature.The results indicated that: the 12 strains had different tolerance ability to drought-hot,such as P5-1,P5-6,P5-8,P6-11,Y8-12,P5-5,Y6-13,P5-7 and lots of stains could resistant temperature 40℃ such as P5-1,P5-2,P5-6,P5-7.In the brouth medium which adding PEG,there were a lot of strains had indicated the strong tolerance ability.From this study of resistance,P5-1,P5-6,P5-7 not only could grow at temperature 40℃ but also in the condition of -8.71×105 Pa and 35℃.
参考文献/References:
[1] 马焕成.干热河谷造林新技术[M].云南:云南科技出版社,2001.1-8.
[2] 韩素芬.我国豆科树种结瘤情况的补充资料[J].南京林业大学学报,1995,19(4):89-91.
[3] 陈文新.土壤中影响根瘤菌存活的因素[J].土壤学进展,1986,(5):17-20.
[4] 关桂兰,郭沛新,王卫卫,等.新疆干旱地区根瘤菌资源研究:Ⅱ根瘤菌抗逆性及生理生化反应特性[J].微生物学报,1992,32(5):346-352.
[5] 陈文新.新疆地区豆科根瘤菌特性的分析(一)[J].土壤肥料,1984,(3):4-7.
[6] 黄玲,石玉瑚,吴祖银.新疆部分豆科植物根瘤菌的某些耐性试验[J].微生物学通报,1990,17(3):130-133.
[7] Burlyn E M,Merrill RK.The Osmotic Potential of Polyethlene Glycol 6000[J].Plant Physiol.1973,51:914-916.
[8] 黄明勇,张小平,李登煜,等.金沙江干热河谷区土著花生根瘤菌耐旱性初步研究[J].应用与环境生物学报,2000,6(3):263-266.
[9] Krieg,N R,J G Holt (Ed).Bergey’s Manual Systematic Bacteriology[M].Volume 1,Tilliams of Wilfins Baltimore,1984.235-244.
[10] 杨苏声,李季伦.快生型大豆根瘤菌的耐盐机制和结瘤性状[J].北京农业大学学报,1988,14(2):143-148.
[11] Vicent JM,Thompson JA,Donovan KO.Death of root-nodule bacteria on drying[J].Aust J Agric Res.,1962,13:258-27.
[12] Fuhuramann J,Davey C B,Wollum A G.Dessication tolerance of Clover Rhizobia in sterile soils[J].Soil Sci Am J.1986,50:639-644.
[13] Van Rensburg HJ,Strijdom BW.Survival of fast-and slow-growing rhizobium ssp.Under conditions of relatively mild dessication[J].Soil Biol Boichem,1980,12:353-356.
相似文献/References:
[1]郭丽琢,何亚慧.豌豆根瘤菌的盐碱适应性鉴定[J].水土保持研究,2009,16(05):270.
GUO Li-zhuo,HE Ya-hui.Adaptability of Pea Rhizobia to NaCl and pH[J].Research of Soil and Water Conservation,2009,16(04):270.
备注/Memo
收稿日期:2005-11-2
基金项目:云南省自然科学基金(编号203305)
作者简介:曾小红(1979- ),女,重庆人,硕士生,主要从事植物生理生态方面的学习与研究;